ID   TM256_MOUSE             Reviewed;         113 AA.
AC   Q5F285;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Transmembrane protein 256;
DE   Flags: Precursor;
GN   Name=Tmem256;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC   Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-43, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=23576753; DOI=10.1073/pnas.1302961110;
RA   Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B.,
RA   Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.;
RT   "Label-free quantitative proteomics of the lysine acetylome in mitochondria
RT   identifies substrates of SIRT3 in metabolic pathways.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TMEM256 family. {ECO:0000305}.
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DR   EMBL; AL603707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC117077; AAI17078.1; -; mRNA.
DR   EMBL; BC117079; AAI17080.1; -; mRNA.
DR   CCDS; CCDS24915.1; -.
DR   RefSeq; NP_081258.1; NM_026982.1.
DR   AlphaFoldDB; Q5F285; -.
DR   STRING; 10090.ENSMUSP00000091608; -.
DR   iPTMnet; Q5F285; -.
DR   PhosphoSitePlus; Q5F285; -.
DR   EPD; Q5F285; -.
DR   jPOST; Q5F285; -.
DR   MaxQB; Q5F285; -.
DR   PaxDb; Q5F285; -.
DR   PeptideAtlas; Q5F285; -.
DR   PRIDE; Q5F285; -.
DR   ProteomicsDB; 262828; -.
DR   Antibodypedia; 76715; 30 antibodies from 9 providers.
DR   Ensembl; ENSMUST00000094065; ENSMUSP00000091608; ENSMUSG00000070394.
DR   GeneID; 69186; -.
DR   KEGG; mmu:69186; -.
DR   UCSC; uc007jry.1; mouse.
DR   CTD; 254863; -.
DR   MGI; MGI:1916436; Tmem256.
DR   VEuPathDB; HostDB:ENSMUSG00000070394; -.
DR   eggNOG; KOG3472; Eukaryota.
DR   GeneTree; ENSGT00390000000334; -.
DR   HOGENOM; CLU_096548_1_2_1; -.
DR   InParanoid; Q5F285; -.
DR   OMA; YCLAGCH; -.
DR   OrthoDB; 1632823at2759; -.
DR   PhylomeDB; Q5F285; -.
DR   TreeFam; TF300271; -.
DR   BioGRID-ORCS; 69186; 2 hits in 74 CRISPR screens.
DR   ChiTaRS; Tmem256; mouse.
DR   PRO; PR:Q5F285; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; Q5F285; protein.
DR   Bgee; ENSMUSG00000070394; Expressed in interventricular septum and 225 other tissues.
DR   ExpressionAtlas; Q5F285; baseline and differential.
DR   Genevisible; Q5F285; MM.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR   InterPro; IPR006696; DUF423.
DR   PANTHER; PTHR43461; PTHR43461; 1.
DR   Pfam; PF04241; DUF423; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..113
FT                   /note="Transmembrane protein 256"
FT                   /id="PRO_0000287171"
FT   TOPO_DOM        30..63
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        85..92
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         43
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:23576753"
SQ   SEQUENCE   113 AA;  11656 MW;  9D309DA2A57827DA CRC64;
     MAGVGAAFRR LGALSGAGAL GLASYGAHGA QFPDAYGKEL FDKANKHHFL HSLALLGVPS
     CRKPVWAGLL LASGTTLFCT SFYYQALSGD TSIQTLGPVG GSLLILGWLA LAF