TM258_BOVIN
ID TM258_BOVIN Reviewed; 79 AA.
AC Q32P84;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Transmembrane protein 258 {ECO:0000250|UniProtKB:P61165};
DE AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258;
DE Short=Oligosaccharyl transferase subunit TMEM258;
GN Name=TMEM258 {ECO:0000250|UniProtKB:P61165};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation (By similarity). N-glycosylation occurs
CC cotranslationally and the complex associates with the Sec61 complex at
CC the channel-forming translocon complex that mediates protein
CC translocation across the endoplasmic reticulum (ER). All subunits are
CC required for a maximal enzyme activity (By similarity). Involved in ER
CC homeostasis in the colonic epithelium (By similarity).
CC {ECO:0000250|UniProtKB:P61165, ECO:0000250|UniProtKB:P61166}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000250|UniProtKB:P61165}.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex (By
CC similarity). OST exists in two different complex forms which contain
CC common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either
CC STT3A or STT3B as catalytic subunits, and form-specific accessory
CC subunits (By similarity). STT3A complex assembly occurs through the
CC formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258,
CC subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and
CC KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2,
CC DAD1, and OST48. The STT3A complex can form stable complexes with the
CC Sec61 complex or with both the Sec61 and TRAP complexes (By
CC similarity). {ECO:0000250|UniProtKB:E2RKN8,
CC ECO:0000250|UniProtKB:P61165}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P61165}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:P61165}. Endoplasmic
CC reticulum {ECO:0000250|UniProtKB:P61165}. Cytoplasm
CC {ECO:0000250|UniProtKB:P61165}.
CC -!- SIMILARITY: Belongs to the OST5 family. {ECO:0000305}.
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DR EMBL; BC108218; AAI08219.1; -; mRNA.
DR RefSeq; NP_001181992.1; NM_001195063.1.
DR AlphaFoldDB; Q32P84; -.
DR SMR; Q32P84; -.
DR STRING; 9913.ENSBTAP00000043516; -.
DR PaxDb; Q32P84; -.
DR PRIDE; Q32P84; -.
DR Ensembl; ENSBTAT00000046195; ENSBTAP00000043516; ENSBTAG00000032557.
DR GeneID; 767958; -.
DR KEGG; bta:767958; -.
DR CTD; 746; -.
DR VEuPathDB; HostDB:ENSBTAG00000032557; -.
DR VGNC; VGNC:36066; TMEM258.
DR eggNOG; KOG4452; Eukaryota.
DR GeneTree; ENSGT00390000010089; -.
DR HOGENOM; CLU_180449_0_0_1; -.
DR InParanoid; Q32P84; -.
DR OMA; MERYVGP; -.
DR OrthoDB; 1627291at2759; -.
DR TreeFam; TF300295; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000009136; Chromosome 29.
DR Bgee; ENSBTAG00000032557; Expressed in semen and 106 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0043227; C:membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0034998; C:oligosaccharyltransferase I complex; IEA:Ensembl.
DR GO; GO:0032991; C:protein-containing complex; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IEA:Ensembl.
DR InterPro; IPR007915; TMEM258/Ost5.
DR PANTHER; PTHR13636; PTHR13636; 1.
DR Pfam; PF05251; Ost5; 1.
PE 3: Inferred from homology;
KW Acetylation; Cytoplasm; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..79
FT /note="Transmembrane protein 258"
FT /id="PRO_0000235832"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P61165"
SQ SEQUENCE 79 AA; 9093 MW; 9395E40132994646 CRC64;
MELEAMSRYT SPVNPAVFPH LTVVLLAIGM FFTAWFFVYE VTSTKYTRDI YKELLISLVA
SLFMGFGVLF LLLWVGIYI
