ID   TM258_CAEBR             Reviewed;          79 AA.
AC   Q60WL8; A8XUQ5;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Transmembrane protein 258 {ECO:0000305};
DE   AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258;
DE            Short=Oligosaccharyl transferase subunit TMEM258;
GN   Name=tmem-258 {ECO:0000312|WormBase:CBG19073};
GN   ORFNames=CBG19073 {ECO:0000312|WormBase:CBG19073};
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000250|UniProtKB:P61165}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000250|UniProtKB:P61165}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000250|UniProtKB:P61165}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the OST5 family. {ECO:0000305}.
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DR   EMBL; HE601047; CAP36380.3; -; Genomic_DNA.
DR   RefSeq; XP_002637372.1; XM_002637326.1.
DR   AlphaFoldDB; Q60WL8; -.
DR   SMR; Q60WL8; -.
DR   STRING; 6238.CBG19073; -.
DR   EnsemblMetazoa; CBG19073.1; CBG19073.1; WBGene00038351.
DR   GeneID; 8579367; -.
DR   KEGG; cbr:CBG_19073; -.
DR   CTD; 8579367; -.
DR   WormBase; CBG19073; CBP19435; WBGene00038351; Cbr-tmem-258.
DR   eggNOG; KOG4452; Eukaryota.
DR   HOGENOM; CLU_180449_0_0_1; -.
DR   InParanoid; Q60WL8; -.
DR   OMA; MERYVGP; -.
DR   OrthoDB; 1627291at2759; -.
DR   UniPathway; UPA00378; -.
DR   Proteomes; UP000008549; Chromosome V.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR   GO; GO:0043227; C:membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0034998; C:oligosaccharyltransferase I complex; IEA:InterPro.
DR   GO; GO:0032991; C:protein-containing complex; IBA:GO_Central.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IEA:InterPro.
DR   InterPro; IPR007915; TMEM258/Ost5.
DR   PANTHER; PTHR13636; PTHR13636; 1.
DR   Pfam; PF05251; Ost5; 1.
PE   3: Inferred from homology;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..79
FT                   /note="Transmembrane protein 258"
FT                   /id="PRO_0000235836"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   79 AA;  8692 MW;  0D2247D4404606BA CRC64;
     MDISKMDRYA APVHFSSLPL LATVLCGVGL LLLAAFTMLQ VTSTKYNRNV FKELFIAATS
     SIFLGFGSVF LLLWVGIYV