TM258_CHICK
ID TM258_CHICK Reviewed; 79 AA.
AC Q76LT9;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Transmembrane protein 258 {ECO:0000250|UniProtKB:P61165};
DE AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258;
DE Short=Oligosaccharyl transferase subunit TMEM258;
DE AltName: Full=Protein NEF1;
GN Name=TMEM258 {ECO:0000250|UniProtKB:P61165}; Synonyms=NEF1;
GN ORFNames=RCJMB04_19i7;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Adachi N., Karanjawala Z.E., Matsuzaki Y., Koyama H., Lieber M.R.;
RT "NEF1, a highly conserved gene located immediately upstream (and in reverse
RT orientation) of the FEN1 gene in several higher vertebrates.";
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P61165}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000250|UniProtKB:P61165}.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P61165}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P61165}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:P61165}. Endoplasmic
CC reticulum {ECO:0000250|UniProtKB:P61165}. Cytoplasm
CC {ECO:0000250|UniProtKB:P61165}.
CC -!- SIMILARITY: Belongs to the OST5 family. {ECO:0000305}.
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DR EMBL; AB073598; BAB91134.1; -; Genomic_DNA.
DR EMBL; AJ720522; CAG32181.1; -; mRNA.
DR RefSeq; NP_001006464.1; NM_001006464.2.
DR AlphaFoldDB; Q76LT9; -.
DR SMR; Q76LT9; -.
DR STRING; 9031.ENSGALP00000005743; -.
DR PaxDb; Q76LT9; -.
DR Ensembl; ENSGALT00000088715; ENSGALP00000060565; ENSGALG00000038292.
DR GeneID; 422964; -.
DR KEGG; gga:422964; -.
DR CTD; 746; -.
DR VEuPathDB; HostDB:geneid_422964; -.
DR eggNOG; KOG4452; Eukaryota.
DR GeneTree; ENSGT00390000010089; -.
DR HOGENOM; CLU_180449_0_0_1; -.
DR InParanoid; Q76LT9; -.
DR OMA; MERYVGP; -.
DR OrthoDB; 1627291at2759; -.
DR PhylomeDB; Q76LT9; -.
DR TreeFam; TF300295; -.
DR UniPathway; UPA00378; -.
DR PRO; PR:Q76LT9; -.
DR Proteomes; UP000000539; Chromosome 5.
DR Bgee; ENSGALG00000038292; Expressed in heart and 13 other tissues.
DR ExpressionAtlas; Q76LT9; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0043227; C:membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0034998; C:oligosaccharyltransferase I complex; IEA:InterPro.
DR GO; GO:0032991; C:protein-containing complex; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IEA:InterPro.
DR InterPro; IPR007915; TMEM258/Ost5.
DR PANTHER; PTHR13636; PTHR13636; 1.
DR Pfam; PF05251; Ost5; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..79
FT /note="Transmembrane protein 258"
FT /id="PRO_0000235837"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 79 AA; 9079 MW; 8105E40132994646 CRC64;
MELEAMSRYT SPVNPAVFPH LTVVLLAIGM FFTAWFFVYE VTSTKYTRDI YKELLISLVA
SLFMGFGVLF LLLWVGIYV
