ID   TM258_DANRE             Reviewed;          79 AA.
AC   Q6PBS6;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Transmembrane protein 258 {ECO:0000250|UniProtKB:P61165};
DE   AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258;
DE            Short=Oligosaccharyl transferase subunit TMEM258;
GN   Name=tmem258 {ECO:0000250|UniProtKB:P61165}; ORFNames=zgc:73269;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000250|UniProtKB:P61165}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000250|UniProtKB:P61165}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000250|UniProtKB:P61165}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P61165}; Multi-
CC       pass membrane protein {ECO:0000250|UniProtKB:P61165}. Endoplasmic
CC       reticulum {ECO:0000250|UniProtKB:P61165}. Cytoplasm
CC       {ECO:0000250|UniProtKB:P61165}.
CC   -!- SIMILARITY: Belongs to the OST5 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC059600; AAH59600.1; -; mRNA.
DR   RefSeq; NP_957063.1; NM_200769.3.
DR   AlphaFoldDB; Q6PBS6; -.
DR   SMR; Q6PBS6; -.
DR   STRING; 7955.ENSDARP00000043171; -.
DR   PaxDb; Q6PBS6; -.
DR   Ensembl; ENSDART00000043172; ENSDARP00000043171; ENSDARG00000078785.
DR   GeneID; 393742; -.
DR   KEGG; dre:393742; -.
DR   CTD; 746; -.
DR   ZFIN; ZDB-GENE-040426-1739; tmem258.
DR   eggNOG; KOG4452; Eukaryota.
DR   GeneTree; ENSGT00390000010089; -.
DR   HOGENOM; CLU_180449_0_0_1; -.
DR   InParanoid; Q6PBS6; -.
DR   OMA; MERYVGP; -.
DR   OrthoDB; 1627291at2759; -.
DR   PhylomeDB; Q6PBS6; -.
DR   TreeFam; TF300295; -.
DR   UniPathway; UPA00378; -.
DR   PRO; PR:Q6PBS6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 25.
DR   Bgee; ENSDARG00000078785; Expressed in intestine and 27 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR   GO; GO:0043227; C:membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0034998; C:oligosaccharyltransferase I complex; IEA:InterPro.
DR   GO; GO:0032991; C:protein-containing complex; IBA:GO_Central.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IEA:InterPro.
DR   InterPro; IPR007915; TMEM258/Ost5.
DR   PANTHER; PTHR13636; PTHR13636; 1.
DR   Pfam; PF05251; Ost5; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..79
FT                   /note="Transmembrane protein 258"
FT                   /id="PRO_0000235833"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   79 AA;  9098 MW;  E86B7A13EF5CB080 CRC64;
     MELEAMTRYT SPVNPAVFPH LTVVLLAIGM FFKAWFFVYE VTSTKYTRDV YKELLIALVA
     SLFMGFGVHF LLLWVGIFV