AC4_TGMVY
ID AC4_TGMVY Reviewed; 85 AA.
AC P0C2W9;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 23-FEB-2022, entry version 41.
DE RecName: Full=Protein AC4;
DE AltName: Full=Protein AL4;
GN ORFNames=AC4, AL4;
OS Tomato golden mosaic virus (strain Yellow vein) (TGMV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Begomovirus.
OX NCBI_TaxID=223341;
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16453557; DOI=10.1002/j.1460-2075.1984.tb02114.x;
RA Hamilton W.D.O., Stein V.E., Coutts R.H.A., Buck K.W.;
RT "Complete nucleotide sequence of the infectious cloned DNA components of
RT tomato golden mosaic virus: potential coding regions and regulatory
RT sequences.";
RL EMBO J. 3:2197-2205(1984).
RN [2]
RP FUNCTION.
RX PubMed=8151290; DOI=10.1099/0022-1317-75-4-721;
RA Groening B.R., Hayes R.J., Buck K.W.;
RT "Simultaneous regulation of tomato golden mosaic virus coat protein and AL1
RT gene expression: expression of the AL4 gene may contribute to suppression
RT of the AL1 gene.";
RL J. Gen. Virol. 75:721-726(1994).
RN [3]
RP FUNCTION.
RX PubMed=8760447; DOI=10.1099/0022-1317-77-8-1947;
RA Pooma W., Petty I.T.D.;
RT "Tomato golden mosaic virus open reading frame AL4 is genetically distinct
RT from its C4 analogue in monopartite geminiviruses.";
RL J. Gen. Virol. 77:1947-1951(1996).
RN [4]
RP FUNCTION.
RX PubMed=9261423; DOI=10.1128/jvi.71.9.6947-6955.1997;
RA Eagle P.A., Hanley-Bowdoin L.;
RT "cis elements that contribute to geminivirus transcriptional regulation and
RT the efficiency of DNA replication.";
RL J. Virol. 71:6947-6955(1997).
RN [5]
RP SUBCELLULAR LOCATION, INTERACTION WITH ARABIDOPSIS THALIANA ASK7/ASK-ETA,
RP MUTAGENESIS OF ARG-44; LEU-45; ARG-49 AND ARG-50, AND PHOSPHORYLATION BY
RP ARABIDOPSIS THALIANA ASK7/ASK-ETA.
RX PubMed=17280695; DOI=10.1016/j.virol.2006.12.034;
RA Piroux N., Saunders K., Page A., Stanley J.;
RT "Geminivirus pathogenicity protein C4 interacts with Arabidopsis thaliana
RT shaggy-related protein kinase AtSKeta, a component of the brassinosteroid
RT signalling pathway.";
RL Virology 362:428-440(2007).
CC -!- FUNCTION: Pathogenicity determinant (By similarity). May act as a
CC suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs. May repress the
CC AL61 promoter. {ECO:0000250, ECO:0000269|PubMed:8151290,
CC ECO:0000269|PubMed:8760447, ECO:0000269|PubMed:9261423}.
CC -!- SUBUNIT: Interacts with Arabidopsis thaliana ASK7/ASK-eta and ASK6/ASK-
CC zeta proteins. {ECO:0000269|PubMed:17280695}.
CC -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000269|PubMed:17280695};
CC Lipid-anchor {ECO:0000269|PubMed:17280695}. Note=Localizes to the cell
CC periphery.
CC -!- PTM: Phosphorylated by Arabidopsis thaliana ASK7/ASK-eta mainly on
CC threonine and serine residues. {ECO:0000269|PubMed:17280695}.
CC -!- SIMILARITY: Belongs to the geminiviridae protein AC4/C4 family.
CC {ECO:0000305}.
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DR EMBL; K02029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR SMR; P0C2W9; -.
DR Proteomes; UP000007405; Genome.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR InterPro; IPR002488; Gemini_C4.
DR Pfam; PF01492; Gemini_C4; 1.
PE 1: Evidence at protein level;
KW Host cell membrane; Host membrane; Host-virus interaction; Lipoprotein;
KW Membrane; Myristate; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..85
FT /note="Protein AC4"
FT /id="PRO_0000287220"
FT LIPID 2
FT /note="N-myristoyl glycine; by host"
FT /evidence="ECO:0000250"
FT MUTAGEN 44
FT /note="R->P: Enhanced pathogenicity on N. benthamiana; when
FT associated with A-45; S-49 and P-50."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 45
FT /note="L->A: Enhanced pathogenicity on N. benthamiana; when
FT associated with P-44; S-49 and P-50."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 49
FT /note="R->S: Enhanced pathogenicity on N. benthamiana; when
FT associated with P-44; A-45 and P-50."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 50
FT /note="R->P: Enhanced pathogenicity on N. benthamiana; when
FT associated with P-44; A-45 and S-49."
FT /evidence="ECO:0000269|PubMed:17280695"
SQ SEQUENCE 85 AA; 9757 MW; F992C93B3EDEFCC5 CRC64;
MGNLTSTCLF SSRENTAAKI NDSSTWYPQQ GQHISIQTFR ELNRLPTSRR TSTKTEILLY
GENSRSTVEV LEEVAKHLTT LQQRR
