TM2NV_SOLLC
ID TM2NV_SOLLC Reviewed; 861 AA.
AC Q5MLE9; Q5UB82;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=ToMV resistant protein Tm-2 netted virescent {ECO:0000303|Ref.1, ECO:0000303|Ref.2};
DE Short=ToMV resistant protein Tm-2nv {ECO:0000303|Ref.1, ECO:0000303|Ref.2};
GN Name=Tm-2nv {ECO:0000303|Ref.1, ECO:0000303|Ref.2, ECO:0000303|Ref.3};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Yukang 2;
RA Jiang G.;
RT "Expression and Virus-Specificity of Tm-2nv in Transgenic Tobacco and
RT Tomato.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Yukang 2;
RA Jiang G.;
RT "Tm-2nv, a virus-resistant gene, cloning and characteristics from BiBAC of
RT a tomato cultivar (Yukang 2).";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RC STRAIN=cv. Yukang 2;
RA Zhou Y., Dai P., Li Y., Jiang G.;
RT "Analysis and comfirmation of a special molecular marker for tomato
RT resistance to ToMV virus.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND SUBUNIT (MICROBIAL INFECTION).
RC STRAIN=cv. Craigella GCR236;
RX PubMed=2535549; DOI=10.2307/3868972;
RA Meshi T., Motoyoshi F., Maeda T., Yoshiwoka S., Watanabe H., Okada Y.;
RT "Mutations in the tobacco mosaic virus 30-kD protein gene overcome Tm-2
RT resistance in tomato.";
RL Plant Cell 1:515-522(1989).
RN [5]
RP GENE FAMILY.
RC STRAIN=cv. Craigella GCR236;
RX PubMed=24173917; DOI=10.1007/bf00221969;
RA Ohmori T., Murata M., Motoyoshi F.;
RT "Identification of RAPD markers linked to the Tm-2 locus in tomato.";
RL Theor. Appl. Genet. 90:307-311(1995).
RN [6]
RP FUNCTION, AND SUBUNIT (MICROBIAL INFECTION).
RX PubMed=29582165; DOI=10.1007/s00705-018-3819-5;
RA Maayan Y., Pandaranayaka E.P.J., Srivastava D.A., Lapidot M., Levin I.,
RA Dombrovsky A., Harel A.;
RT "Using genomic analysis to identify tomato Tm-2 resistance-breaking
RT mutations and their underlying evolutionary path in a new and emerging
RT tobamovirus.";
RL Arch. Virol. 163:1863-1875(2018).
CC -!- FUNCTION: Inhibitor of viral mouvements which confers resistance to
CC some tobamoviruses including tomato mosaic virus (ToMV) (e.g. isolate L
CC and W3) and tobacco mosaic virus (TMV), but not to resistance-breaking
CC isolates (e.g. Ltbl) ToMV and tomato brown rugose fruit virus (ToBRFV)
CC (PubMed:2535549, PubMed:29582165). Elicits a hypersensitive reaction in
CC response to avirulent (Avr) movement proteins from resistance inducing
CC tobamoviruses (e.g. ToMV and TMV) strains, thus leading to programmed
CC cell death (By similarity). {ECO:0000250|UniProtKB:Q71BG9,
CC ECO:0000269|PubMed:2535549, ECO:0000269|PubMed:29582165}.
CC -!- SUBUNIT: (Microbial infection) Interacts with tobamoviruses mouvement
CC protein at the plasma membrane; this interaction triggers defense
CC responses leading to programmed cell death.
CC {ECO:0000305|PubMed:2535549, ECO:0000305|PubMed:29582165}.
CC -!- SUBUNIT: Binds to HSP90 proteins; this interaction seems required for
CC defense responses toward tobamoviruses. {ECO:0000250|UniProtKB:Q71BG9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q71BG9};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:Q71BG9}; Cytoplasmic
CC side {ECO:0000250|UniProtKB:Q71BG9}.
CC -!- MISCELLANEOUS: The Tm-2, Tm-2(2) and Tm-2nv alleles present in the
CC tomato mosaic virus (ToMV/TMV)-resistant tomato cv. Craigella isolates
CC GCR236 (AC Q71BH0), cv. Craigella GCR267 (AC Q71BG9) and cv. Yukang 2
CC (AC Q5MLE9), respectively confers resistance to ToMV but not the tm-2
CC allele present in the ToMV-susceptible tomato cv. Craigella isolate
CC GCR26 (AC Q71BH1). {ECO:0000269|PubMed:2535549}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
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DR EMBL; AY742887; AAV87531.1; -; Genomic_DNA.
DR EMBL; HM027885; AEA51264.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5MLE9; -.
DR SMR; Q5MLE9; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; Q5MLE9; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IDA:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR CDD; cd14798; RX-CC_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038005; RX-like_CC.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Coiled coil; Host-virus interaction;
KW Hypersensitive response; Leucine-rich repeat; Membrane; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..861
FT /note="ToMV resistant protein Tm-2 netted virescent"
FT /id="PRO_0000448717"
FT DOMAIN 162..388
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 225..248
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 305..327
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 388..411
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 449..472
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 510..536
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 585..608
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 609..631
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 652..680
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 689..710
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 712..735
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 736..758
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 784..807
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 810..835
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT COILED 63..83
FT /evidence="ECO:0000255"
FT BINDING 185..192
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 861 AA; 98610 MW; 86E98E8FCB0FE494 CRC64;
MAEILLTSVI NKSVEIAGNL LIQEGKRLYW LKEDIDWLQR EMRHIRSYVD NAKAKEAGGD
SRVKNLLKDI QELAGDVEDL LDDFLPKIQQ SNKFNYCLKR SSFADEFAME IEKIKRRVVD
IDRIRKTYNI IDTDNNNDDC VLLDRRRLFL HADETEIIGL DDDFNMLQAK LLNQDLHYGV
VSIVGMPGLG KTTLAKKLYR LIRDQFECSG LVYVSQQPRA SEILLDIAKQ IGLTEQKMKE
NLEDNLRSLL KIKRYVFLLD DVWDVEIWDD LKLVLPECDS KVGSRIIITS RNSNVGRYIG
GESSLHALQP LESEKSFELF TKKIFNFDDN NSWANASPDL VNIGRNIVGR CGGIPLAIVV
TAGMLRARER TEHAWNRVLE SMGHKVQDGC AKVLALSYND LPIASRPCFL YFGLYPEDHE
IRAFDLINMW IAEKFIVVNS GNRREAEDLA EDVLNDLVSR NLIQLAKRTY NGRISSCRIH
DLLHSLCVDL AKESNFFHTA HDAFGDPGNV ARLRRITFYS DNVMIEFFRS NPKLEKLRVL
FCFAKDPSIF SHMAYFDFKL LHTLVVVMSQ SFQAYVTIPS KFGNMTCLRY LRLEGNICGK
LPNSIVKLTR LETIDIDRRS LIQPPSGVWE SKHLRHLCYR DYGQACNSCF SISSFYPNIY
SLHPNNLQTL MWIPDKFFEP RLLHRLINLR KLGILGVSNS TVKMLSIFSP VLKALEVLKL
SFSSDPSEQI KLSSYPHIAK LHLNVNRTMA LNSQSFPPNL IKLTLANFTV DRYILAVLKT
FPKLRKLKMF ICKYNEEKMA LSGEANGYSF PQLEVLHIHS PNGLSEVTCT DDVSMPKLKK
LLLTGFHCGI SLSERLKKLS K
