TM2R_SOLLC
ID TM2R_SOLLC Reviewed; 861 AA.
AC Q71BH0; C3UZH8; C3UZI4; Q5UB82;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=ToMV resistance protein Tm-2(GCR236) {ECO:0000303|PubMed:14558663};
DE AltName: Full=Disease resistance protein Tm-2 {ECO:0000303|Ref.3};
GN Name=Tm-2 {ECO:0000303|PubMed:14558663, ECO:0000303|Ref.2};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Craigella GCR236;
RX PubMed=14558663; DOI=10.1023/a:1025434519282;
RA Lanfermeijer F.C., Dijkhuis J., Sturre M.J.G., de Haan P., Hille J.;
RT "Cloning and characterization of the durable tomato mosaic virus resistance
RT gene Tm-2(2) from Lycopersicon esculentum.";
RL Plant Mol. Biol. 52:1037-1049(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RC STRAIN=cv. Craigella GCR236;
RA Lanfermeijer F.C., ten Hoopen F., Hille J.;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-423 AND 594-827.
RC STRAIN=cv. LA3432, and cv. LA3433;
RA Shi A., Vierling R., Grazzini R., Hogue P., Miller K.;
RT "Development of SNP markers for disease resistance genes in tomato.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR236;
RX PubMed=2535549; DOI=10.2307/3868972;
RA Meshi T., Motoyoshi F., Maeda T., Yoshiwoka S., Watanabe H., Okada Y.;
RT "Mutations in the tobacco mosaic virus 30-kD protein gene overcome Tm-2
RT resistance in tomato.";
RL Plant Cell 1:515-522(1989).
RN [5]
RP FUNCTION.
RX PubMed=1730937; DOI=10.1099/0022-1317-73-1-165;
RA Calder V.L., Palukaitis P.;
RT "Nucleotide sequence analysis of the movement genes of resistance breaking
RT strains of tomato mosaic virus.";
RL J. Gen. Virol. 73:165-168(1992).
RN [6]
RP GENE FAMILY.
RC STRAIN=cv. Craigella GCR236;
RX PubMed=24173917; DOI=10.1007/bf00221969;
RA Ohmori T., Murata M., Motoyoshi F.;
RT "Identification of RAPD markers linked to the Tm-2 locus in tomato.";
RL Theor. Appl. Genet. 90:307-311(1995).
RN [7]
RP GENE FAMILY.
RX PubMed=24710868; DOI=10.1007/s001220050745;
RA Ohmori T., Murata M., Motoyoshi F.;
RT "Characterization of disease resistance gene-like sequences in near-
RT isogenic lines of tomato.";
RL Theor. Appl. Genet. 96:331-338(1998).
RN [8]
RP FUNCTION, AND SUBUNIT (MICROBIAL INFECTION).
RC STRAIN=cv. Craigella GCR236;
RX PubMed=15290376; DOI=10.1007/s00705-004-0312-0;
RA Weber H., Ohnesorge S., Silber M.V., Pfitzner A.J.P.;
RT "The Tomato mosaic virus 30 kDa movement protein interacts differentially
RT with the resistance genes Tm-2 and Tm-2(2).";
RL Arch. Virol. 149:1499-1514(2004).
RN [9]
RP FUNCTION, MISCELLANEOUS, AND GENE FAMILY.
RC STRAIN=cv. Craigella GCR236;
RX PubMed=16172136; DOI=10.1093/jxb/eri288;
RA Lanfermeijer F.C., Warmink J., Hille J.;
RT "The products of the broken Tm-2 and the durable Tm-2(2) resistance genes
RT from tomato differ in four amino acids.";
RL J. Exp. Bot. 56:2925-2933(2005).
RN [10]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR236, and cv. Craigella GCR254;
RX PubMed=17238011; DOI=10.1007/s00705-006-0915-8;
RA Strasser M., Pfitzner A.J.P.;
RT "The double-resistance-breaking Tomato mosaic virus strain ToMV1-2 contains
RT two independent single resistance-breaking domains.";
RL Arch. Virol. 152:903-914(2007).
RN [11]
RP FUNCTION, AND SUBUNIT (MICROBIAL INFECTION).
RX PubMed=21310506; DOI=10.1016/j.jplph.2011.01.002;
RA Kobayashi M., Yamamoto-Katou A., Katou S., Hirai K., Meshi T., Ohashi Y.,
RA Mitsuhara I.;
RT "Identification of an amino acid residue required for differential
RT recognition of a viral movement protein by the Tomato mosaic virus
RT resistance gene Tm-2(2).";
RL J. Plant Physiol. 168:1142-1145(2011).
RN [12]
RP FUNCTION.
RC STRAIN=cv. Moperou;
RX PubMed=29018314; DOI=10.5423/ppj.nt.04.2017.0082;
RA Sihelska N., Vozarova Z., Predajna L., Soltys K., Hudcovicova M.,
RA Mihalik D., Kraic J., Mrkvova M., Kudela O., Glasa M.;
RT "Experimental infection of different tomato genotypes with tomato mosaic
RT virus led to a low viral population heterogeneity in the capsid protein
RT encoding region.";
RL Plant Pathol. J. 33:508-513(2017).
RN [13]
RP FUNCTION.
RC STRAIN=cv. Ikram, cv. Mocimor, cv. Momor, cv. Momor verte, cv. Moneymaker,
RC cv. Moperou, cv. Mose, and cv. Vendor;
RX PubMed=28107419; DOI=10.1371/journal.pone.0170429;
RA Luria N., Smith E., Reingold V., Bekelman I., Lapidot M., Levin I.,
RA Elad N., Tam Y., Sela N., Abu-Ras A., Ezra N., Haberman A., Yitzhak L.,
RA Lachman O., Dombrovsky A.;
RT "A new Israeli tobamovirus isolate infects tomato plants harboring Tm-2(2)
RT resistance genes.";
RL PLoS ONE 12:E0170429-E0170429(2017).
RN [14]
RP FUNCTION.
RX PubMed=29582165; DOI=10.1007/s00705-018-3819-5;
RA Maayan Y., Pandaranayaka E.P.J., Srivastava D.A., Lapidot M., Levin I.,
RA Dombrovsky A., Harel A.;
RT "Using genomic analysis to identify tomato Tm-2 resistance-breaking
RT mutations and their underlying evolutionary path in a new and emerging
RT tobamovirus.";
RL Arch. Virol. 163:1863-1875(2018).
CC -!- FUNCTION: Inhibitor of viral mouvements which confers resistance to
CC some tobamoviruses including tomato mosaic virus (ToMV) (e.g. isolates
CC L, W3 and SL-1) and tobacco mosaic virus (TMV), but not to resistance-
CC breaking isolates (e.g. B7, LT1, LII, Ltbl, ToMV2, and ToMV1-2) ToMV
CC and tomato brown rugose fruit virus (ToBRFV) (PubMed:17238011,
CC PubMed:2535549, PubMed:1730937, PubMed:15290376, PubMed:16172136,
CC PubMed:21310506, PubMed:29582165, PubMed:28107419). Elicits a
CC hypersensitive reaction in response to avirulent (Avr) movement
CC proteins from resistance inducing tobamoviruses (e.g. ToMV and TMV)
CC strains, thus leading to programmed cell death (PubMed:15290376,
CC PubMed:21310506). {ECO:0000269|PubMed:15290376,
CC ECO:0000269|PubMed:16172136, ECO:0000269|PubMed:17238011,
CC ECO:0000269|PubMed:1730937, ECO:0000269|PubMed:21310506,
CC ECO:0000269|PubMed:2535549, ECO:0000269|PubMed:28107419,
CC ECO:0000269|PubMed:29582165}.
CC -!- SUBUNIT: (Microbial infection) Interacts with tobamoviruses mouvement
CC protein at the plasma membrane; this interaction triggers defense
CC responses leading to programmed cell death.
CC {ECO:0000305|PubMed:15290376, ECO:0000305|PubMed:21310506}.
CC -!- SUBUNIT: Binds to HSP90 proteins; this interaction seems required for
CC defense responses toward tobamoviruses. {ECO:0000250|UniProtKB:Q71BG9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q71BG9};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:Q71BG9}; Cytoplasmic
CC side {ECO:0000250|UniProtKB:Q71BG9}.
CC -!- MISCELLANEOUS: Transgenic tobacco (cv. SR1) plants expressing Tm-2
CC exhibit resistance to tobacco mosaic virus (TMV) and tomato mosaic
CC virus (ToMV), tobamoviruses that can multiply in tobacco (cv. SR1).
CC {ECO:0000269|PubMed:16172136}.
CC -!- MISCELLANEOUS: The Tm-2, Tm-2(2) and Tm-2nv alleles present in the
CC tomato mosaic virus (ToMV/TMV)-resistant tomato cv. Craigella isolates
CC GCR236 (AC Q71BH0), cv. Craigella GCR267 (AC Q71BG9) and cv. Yukang 2
CC (AC Q5MLE9), respectively confers resistance to ToMV but not the tm-2
CC allele present in the ToMV-susceptible tomato cv. Craigella isolate
CC GCR26 (AC Q71BH1). {ECO:0000269|PubMed:14558663}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
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DR EMBL; AF536200; AAQ10735.1; -; Genomic_DNA.
DR EMBL; AY765394; AAV39528.1; -; Genomic_DNA.
DR EMBL; FJ817598; ACO52371.1; -; Genomic_DNA.
DR EMBL; FJ817604; ACO52377.1; -; Genomic_DNA.
DR EMBL; FJ817605; ACO52378.1; -; Genomic_DNA.
DR AlphaFoldDB; Q71BH0; -.
DR SMR; Q71BH0; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; Q71BH0; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IDA:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IDA:UniProtKB.
DR CDD; cd14798; RX-CC_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038005; RX-like_CC.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Coiled coil; Host-virus interaction;
KW Hypersensitive response; Leucine-rich repeat; Membrane; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..861
FT /note="ToMV resistance protein Tm-2(GCR236)"
FT /id="PRO_0000448715"
FT DOMAIN 162..388
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 225..248
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 305..327
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 388..411
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 449..472
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 510..536
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 585..608
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 609..631
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 652..680
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 689..710
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 712..735
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 736..758
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 784..810
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 811..835
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT COILED 63..83
FT /evidence="ECO:0000255"
FT BINDING 185..192
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 861 AA; 98767 MW; 797A6C36F09C005D CRC64;
MAEILLTSVI NKSVEIAGNL LIQEGKRLYW LKEDIDWLQR EMRHIRSYVD NAKAKEAGGD
SRVKNLLKDI QELAGDVEDL LDDFLPKIQQ SNKFNYCLKR SSFADEFAME IEKIKRRVVD
IDRIRKTYNI IDTDNNNDDC VLLDRRRLFL HADETEIIGL DDDFNMLQAK LLNQDLHYGV
VSIVGMPGLG KTTLAKKLYR LIRDQFECSG LVYVSQQPRA SEILLDIAKQ IGLTEQKMKE
NLEDNLRSLL KIKRYVFLLD DIWDVEIWDD LKLVLPECDS KVGSRIIITS RNSNVGRYIG
GESSLHALQP LESEKSFELF TKKIFNFDDN NSWANASPDL VNIGRNIVGR CGGIPLAIVV
TAGMLRARER TEHAWNRVLE SMGHKVQDGC AKVLALSYND LPIASRPCFL YFGLYPEDHE
IRAFDLINMW IAEKFIVVNS GNRREAEDLA EDVLNDLVSR NLIQLAKRTY NGRISSCRIH
DLLHSLCVDL AKESNFFHTA HDAFGDPGNV ARLRRITFYS DNVMIEFFRS NPKLEKLRVL
FCFAKDPSIF SHMAYFDFKL LHTLVVVMSQ SFQAYVTIPS KFGNMTCLRY LRLEGNICGK
LPNSIVKLTR LETIDIDRRS LIQPPSGVWE SKHLRHLCYR DYGQACNSCF SISSFYPNIY
SLHPNNLQTL MWIPDKFFEP RLLHRLINLR KLGILGVSNS TVKMLSIFSP VLKALEVLKL
SFSSDPSEQI KLSSYPHIAK LHLNVNRTMA LNSQSFPPNL IKLTLANFTV DRYILAVLKT
FPKLRKLKMF ICKYNEEKMD LSGEANGYSF PQLEVLHIHS PNGLSEVTCT DDVSMPKLKK
LLLTGFHCRI SLSERLKKLS K
