ID   TM48_ECOLX              Reviewed;         235 AA.
AC   P0DV85;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec48 transmembrane protein {ECO:0000303|PubMed:33157039};
GN   ORFNames=ERS085376_00285 {ECO:0000303|Ref.2}, Ga0119542_1001286;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:U66703}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35377 / ECOR 58;
RX   PubMed=8878541; DOI=10.1006/bbrc.1996.1534;
RA   Mao J.R., Inouye M., Inouye S.;
RT   "An unusual bacterial reverse transcriptase having LVDD in the YXDD box
RT   from Escherichia coli.";
RL   Biochem. Biophys. Res. Commun. 227:489-493(1996).
RN   [2] {ECO:0000312|EMBL:CTR27284.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=700337;
RG   Pathogen Informatics;
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, IDENTIFICATION AS A RETRON, DOMAIN, AND
RP   MUTAGENESIS OF 3-ALA--TYR-85.
RC   STRAIN=700337;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Membrane component of antiviral defense system Retron Ec48,
CC       composed of a non-coding RNA (ncRNA), a reverse transcriptase (RT) and
CC       this membrane protein. Expression of this retron confers protection
CC       against bacteriophages lambda, T2, T4, T5 and T7. At multiplicity of
CC       infection (MOI) of 0.02 cultures grow normally when infected with
CC       lambda without collapsing, at MOI 2 cultures enter growth stasis. At
CC       MOI 3 cell membranes are permeabilized within 15 minutes of infection
CC       but do not lyse, suggesting the phage are not able to finish a
CC       replication cycle. Antiviral defense is suppressed by mutations that
CC       knockout the lambda gam expression or phage T7 gp5.9 expression; both
CC       viral genes inhibit host RecBCD (PubMed:33157039). The Ec48 retron may
CC       sense the integrity of the RecBCD enzyme; when RecBCD is perturbed by
CC       viral proteins the Ec48 effector (the membrane protein) is activated,
CC       leading to abortive infection and bacterial growth arrest (Probable).
CC       {ECO:0000269|PubMed:33157039, ECO:0000305|PubMed:33157039}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The N-terminal transmembrane region is required for protection
CC       against bacteriophage infection. {ECO:0000269|PubMed:33157039}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=U66703; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U66703; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CXZM01000001; CTR27284.1; -; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..235
FT                   /note="Retron Ec48 transmembrane protein"
FT                   /id="PRO_0000456034"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         3..85
FT                   /note="Missing: No longer protects against infection by
FT                   lambda, T2, T4, T5 or T7, cells are not permeabilized and
FT                   lyse 45 minutes after infection with lambda."
FT                   /evidence="ECO:0000269|PubMed:33157039"
FT   CONFLICT        21
FT                   /note="I -> S (in Ref. 1; U66703)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        122
FT                   /note="K -> N (in Ref. 1; U66703)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        136
FT                   /note="A -> G (in Ref. 1; U66703)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        150..153
FT                   /note="Missing (in Ref. 1; U66703)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   235 AA;  27237 MW;  6B3F3F4240803DE7 CRC64;
     MNANIRLLKY IVGVSSALFL IFSLISLFET IQNEKLYERD ICFDSQCLKF FAEKTSGIVM
     YFQAFGWLIT TFVTVFGVMI ALMTYNAGVK NNNNSNYTSH LTMFREFASA ELTKRSSIYP
     EKVNFFRWYR VMFPEAQGGD ISVSRDYLEI ISRIKCVIEE ANAHITEENK DYKYKTHQRK
     MMAVLDEIGI SISNGPKNIF IEVESQILDY IDTINLSFCH SSSVIELSRV KRKYI