TM50B_HUMAN
ID TM50B_HUMAN Reviewed; 158 AA.
AC P56557; B2R4L4; D3DSF1; O60537; Q5PY47;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transmembrane protein 50B;
DE AltName: Full=HCV p7-trans-regulated protein 3;
GN Name=TMEM50B; Synonyms=C21orf4; ORFNames=UNQ167/PRO193;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=10077530;
RA Reboul J., Gardiner K., Monneron D., Uze G., Lutfalla G.;
RT "Comparative genomic analysis of the interferon/interleukin-10 receptor
RT gene cluster.";
RL Genome Res. 9:242-250(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Guo J., Cheng J., Ji D., Zhao L.-F., Zhang L.-Y.;
RT "Screening and identification of genes trans-regulated by HCV p7 protein
RT with microarray assay.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Retina;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- SUBUNIT: May form homotrimers or homodimers.
CC {ECO:0000250|UniProtKB:Q9D1X9}.
CC -!- INTERACTION:
CC P56557; Q86WK6: AMIGO1; NbExp=3; IntAct=EBI-12366453, EBI-19125216;
CC P56557; Q07817: BCL2L1; NbExp=3; IntAct=EBI-12366453, EBI-78035;
CC P56557; P58418: CLRN1; NbExp=3; IntAct=EBI-12366453, EBI-17274839;
CC P56557; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-12366453, EBI-6942903;
CC P56557; Q92838: EDA; NbExp=3; IntAct=EBI-12366453, EBI-529425;
CC P56557; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-12366453, EBI-18304435;
CC P56557; Q14802-3: FXYD3; NbExp=3; IntAct=EBI-12366453, EBI-12175685;
CC P56557; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-12366453, EBI-13345167;
CC P56557; Q8TED1: GPX8; NbExp=3; IntAct=EBI-12366453, EBI-11721746;
CC P56557; Q95HB9: HLA-DPA1; NbExp=3; IntAct=EBI-12366453, EBI-17686856;
CC P56557; O15243: LEPROT; NbExp=3; IntAct=EBI-12366453, EBI-15672507;
CC P56557; O95214: LEPROTL1; NbExp=3; IntAct=EBI-12366453, EBI-750776;
CC P56557; Q8TAF8: LHFPL5; NbExp=3; IntAct=EBI-12366453, EBI-2820517;
CC P56557; O76036-6: NCR1; NbExp=3; IntAct=EBI-12366453, EBI-19157577;
CC P56557; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-12366453, EBI-17247926;
CC P56557; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-12366453, EBI-18159983;
CC P56557; Q7RTY0: SLC16A13; NbExp=3; IntAct=EBI-12366453, EBI-12243266;
CC P56557; Q9NQQ7-3: SLC35C2; NbExp=3; IntAct=EBI-12366453, EBI-17295964;
CC P56557; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-12366453, EBI-8638294;
CC P56557; Q4KMG9: TMEM52B; NbExp=3; IntAct=EBI-12366453, EBI-18178701;
CC P56557; Q6PEY1: TMEM88; NbExp=3; IntAct=EBI-12366453, EBI-17198826;
CC P56557; Q9Y320: TMX2; NbExp=3; IntAct=EBI-12366453, EBI-6447886;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9D1X9}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9D1X9};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the UPF0220 family. {ECO:0000305}.
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DR EMBL; AF045606; AAC05974.2; -; mRNA.
DR EMBL; AF039906; AAC05090.1; -; Genomic_DNA.
DR EMBL; AY820138; AAV68502.1; -; mRNA.
DR EMBL; AY358634; AAQ88997.1; -; mRNA.
DR EMBL; AK311870; BAG34811.1; -; mRNA.
DR EMBL; BX537378; CAD97620.1; -; mRNA.
DR EMBL; CH471079; EAX09832.1; -; Genomic_DNA.
DR EMBL; CH471079; EAX09833.1; -; Genomic_DNA.
DR EMBL; BC000569; AAH00569.1; -; mRNA.
DR CCDS; CCDS13625.1; -.
DR RefSeq; NP_006125.2; NM_006134.6.
DR RefSeq; XP_011528048.1; XM_011529746.1.
DR AlphaFoldDB; P56557; -.
DR BioGRID; 107212; 24.
DR IntAct; P56557; 22.
DR STRING; 9606.ENSP00000439768; -.
DR TCDB; 9.B.199.1.2; the 4 tms pf05225 (pf0225) family.
DR iPTMnet; P56557; -.
DR SwissPalm; P56557; -.
DR BioMuta; TMEM50B; -.
DR DMDM; 12643476; -.
DR jPOST; P56557; -.
DR MassIVE; P56557; -.
DR MaxQB; P56557; -.
DR PaxDb; P56557; -.
DR PeptideAtlas; P56557; -.
DR PRIDE; P56557; -.
DR ProteomicsDB; 56927; -.
DR Antibodypedia; 42040; 37 antibodies from 18 providers.
DR DNASU; 757; -.
DR Ensembl; ENST00000420455.5; ENSP00000397773.1; ENSG00000142188.17.
DR Ensembl; ENST00000542230.7; ENSP00000439768.2; ENSG00000142188.17.
DR Ensembl; ENST00000571778.5; ENSP00000458900.1; ENSG00000263160.6.
DR Ensembl; ENST00000573374.5; ENSP00000460121.1; ENSG00000263160.6.
DR GeneID; 757; -.
DR KEGG; hsa:757; -.
DR MANE-Select; ENST00000542230.7; ENSP00000439768.2; NM_006134.7; NP_006125.2.
DR UCSC; uc061zqd.1; human.
DR CTD; 757; -.
DR DisGeNET; 757; -.
DR GeneCards; TMEM50B; -.
DR HGNC; HGNC:1280; TMEM50B.
DR HPA; ENSG00000142188; Low tissue specificity.
DR MIM; 617894; gene.
DR neXtProt; NX_P56557; -.
DR OpenTargets; ENSG00000142188; -.
DR PharmGKB; PA25835; -.
DR VEuPathDB; HostDB:ENSG00000142188; -.
DR eggNOG; KOG3393; Eukaryota.
DR GeneTree; ENSGT00940000155131; -.
DR HOGENOM; CLU_096876_1_0_1; -.
DR InParanoid; P56557; -.
DR OMA; LWIMFAD; -.
DR OrthoDB; 1422977at2759; -.
DR PhylomeDB; P56557; -.
DR TreeFam; TF300282; -.
DR PathwayCommons; P56557; -.
DR SignaLink; P56557; -.
DR BioGRID-ORCS; 757; 25 hits in 1042 CRISPR screens.
DR ChiTaRS; TMEM50B; human.
DR GenomeRNAi; 757; -.
DR Pharos; P56557; Tdark.
DR PRO; PR:P56557; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; P56557; protein.
DR Bgee; ENSG00000142188; Expressed in right lobe of thyroid gland and 93 other tissues.
DR ExpressionAtlas; P56557; baseline and differential.
DR Genevisible; P56557; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:LIFEdb.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:UniProtKB.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR InterPro; IPR007919; UPF0220.
DR PANTHER; PTHR13180; PTHR13180; 1.
DR Pfam; PF05255; UPF0220; 1.
PE 1: Evidence at protein level;
KW Acetylation; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..158
FT /note="Transmembrane protein 50B"
FT /id="PRO_0000174183"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 158 AA; 17936 MW; 515ABB6AAA817DEB CRC64;
MAGFLDNFRW PECECIDWSE RRNAVASVVA GILFFTGWWI MIDAAVVYPK PEQLNHAFHT
CGVFSTLAFF MINAVSNAQV RGDSYESGCL GRTGARVWLF IGFMLMFGSL IASMWILFGA
YVTQNTDVYP GLAVFFQNAL IFFSTLIYKF GRTEELWT
