TM50B_MOUSE
ID TM50B_MOUSE Reviewed; 158 AA.
AC Q9D1X9; Q3UZ98; Q8N7M9;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transmembrane protein 50B;
GN Name=Tmem50b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Bone marrow, Corpora quadrigemina, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Suzuki O., Sasaki N., Aotsuka S., Shoji T., Ichihara T., Shiohata N.,
RA Matsumoto K., Hirano M., Sano S., Nomura R., Yoshikawa Y., Matsumura Y.,
RA Moriya S., Chiba E., Momiyama H., Onogawa S., Kaeriyama S., Satoh N.,
RA Matsunawa H., Takahashi E., Kataoka R., Kuga N., Kuroda A., Satoh I.,
RA Kamata K., Takami S., Terashima Y., Watanabe M., Sugiyama T., Irie R.,
RA Otsuki T., Sato H., Ota T., Wakamatsu A., Ishii S., Yamamoto J., Isono Y.,
RA Kawai-Hio Y., Saito K., Nishikawa T., Kimura K., Yamashita H., Matsuo K.,
RA Nakamura Y., Sekine M., Kikuchi H., Kanda K., Wagatsuma M., Murakawa K.,
RA Kanehori K., Takahashi-Fujii A., Oshima A., Sugiyama A., Kawakami B.,
RA Suzuki Y., Sugano S., Nagahari K., Masuho Y., Nagai K., Isogai T.;
RT "NEDO cDNA sequencing project.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=18541381; DOI=10.1016/j.neuroscience.2008.01.089;
RA Moldrich R.X., Laine J., Visel A., Beart P.M., Laffaire J., Rossier J.,
RA Potier M.C.;
RT "Transmembrane protein 50b (C21orf4), a candidate for Down syndrome
RT neurophenotypes, encodes an intracellular membrane protein expressed in the
RT rodent brain.";
RL Neuroscience 154:1255-1266(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: May form homotrimers or homodimers.
CC {ECO:0000269|PubMed:18541381}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:18541381}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:18541381};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in brain, heart and testis (at protein
CC level). In the cerebellum, has particularly strong expression in
CC Bergmann astroglial cells (at protein level).
CC {ECO:0000269|PubMed:18541381}.
CC -!- DEVELOPMENTAL STAGE: Detected in cortical plate, trigeminal ganglion,
CC dorsal root ganglia and the spinal cord at embryonic stage 14.5 dpc.
CC Widely expressed in brain at postnatal day 7 including cortex,
CC olfactory bulb, hippocampus and cerebellum. Expressed throughout layers
CC II-VI of the cortex. In the olfactory bulb, has strongest expression in
CC glomerular and mitral cell layers. In hippocampus, localizes to CA1,
CC CA2, CA3 and dentate gyrus regions. In cerebellum, mainly expressed in
CC the internal granule cell layer and the Purkinje cell layer.
CC {ECO:0000269|PubMed:18541381}.
CC -!- SIMILARITY: Belongs to the UPF0220 family. {ECO:0000305}.
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DR EMBL; AK020977; BAB32266.1; -; mRNA.
DR EMBL; AK098154; BAC05243.1; -; mRNA.
DR EMBL; AK133966; BAE21959.1; -; mRNA.
DR EMBL; AK150773; BAE29839.1; -; mRNA.
DR EMBL; AK151796; BAE30696.1; -; mRNA.
DR EMBL; AK152190; BAE31021.1; -; mRNA.
DR EMBL; AK152767; BAE31480.1; -; mRNA.
DR EMBL; AK155082; BAE33034.1; -; mRNA.
DR EMBL; AK170684; BAE41957.1; -; mRNA.
DR EMBL; AK172335; BAE42953.1; -; mRNA.
DR EMBL; BC004841; AAH04841.1; -; mRNA.
DR CCDS; CCDS28328.1; -.
DR RefSeq; NP_084294.1; NM_030018.3.
DR AlphaFoldDB; Q9D1X9; -.
DR STRING; 10090.ENSMUSP00000023686; -.
DR SwissPalm; Q9D1X9; -.
DR MaxQB; Q9D1X9; -.
DR PaxDb; Q9D1X9; -.
DR PRIDE; Q9D1X9; -.
DR ProteomicsDB; 260704; -.
DR Antibodypedia; 42040; 37 antibodies from 18 providers.
DR DNASU; 77975; -.
DR Ensembl; ENSMUST00000023686; ENSMUSP00000023686; ENSMUSG00000022964.
DR GeneID; 77975; -.
DR KEGG; mmu:77975; -.
DR UCSC; uc007zxr.1; mouse.
DR CTD; 757; -.
DR MGI; MGI:1925225; Tmem50b.
DR VEuPathDB; HostDB:ENSMUSG00000022964; -.
DR eggNOG; KOG3393; Eukaryota.
DR GeneTree; ENSGT00940000155131; -.
DR HOGENOM; CLU_096876_1_0_1; -.
DR InParanoid; Q9D1X9; -.
DR OMA; LWIMFAD; -.
DR OrthoDB; 1422977at2759; -.
DR PhylomeDB; Q9D1X9; -.
DR TreeFam; TF300282; -.
DR BioGRID-ORCS; 77975; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Tmem50b; mouse.
DR PRO; PR:Q9D1X9; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9D1X9; protein.
DR Bgee; ENSMUSG00000022964; Expressed in cortical plate and 337 other tissues.
DR ExpressionAtlas; Q9D1X9; baseline and differential.
DR Genevisible; Q9D1X9; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR InterPro; IPR007919; UPF0220.
DR PANTHER; PTHR13180; PTHR13180; 1.
DR Pfam; PF05255; UPF0220; 1.
PE 1: Evidence at protein level;
KW Acetylation; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P56557"
FT CHAIN 2..158
FT /note="Transmembrane protein 50B"
FT /id="PRO_0000174184"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P56557"
SQ SEQUENCE 158 AA; 17948 MW; 4DC5B59ABA80719A CRC64;
MAGFLDNFRW PECECIDWSE RRNTVASVVA GILFFTGWWI MIDAAVVYPK PEQLNHAFHT
CGVFSTLAFF MINAVSNAQV RGDSYESGCL GRTGARVWLF IGFMLMFGSL IASMWILFGA
YVTQNIDVYP GLAVFFQNAL IFFSTLIYKF GRTEELWA
