TM86B_HUMAN
ID TM86B_HUMAN Reviewed; 226 AA.
AC Q8N661;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Lysoplasmalogenase;
DE EC=3.3.2.2 {ECO:0000269|PubMed:21515882};
DE AltName: Full=Transmembrane protein 86B;
GN Name=TMEM86B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-199.
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
RP REGULATION, DIMERIZATION, AND SUBCELLULAR LOCATION.
RX PubMed=21515882; DOI=10.1074/jbc.m111.247163;
RA Wu L.C., Pfeiffer D.R., Calhoon E.A., Madiai F., Marcucci G., Liu S.,
RA Jurkowitz M.S.;
RT "Purification, identification, and cloning of lysoplasmalogenase, the
RT enzyme that catalyzes hydrolysis of the vinyl ether bond of
RT lysoplasmalogen.";
RL J. Biol. Chem. 286:24916-24930(2011).
CC -!- FUNCTION: Enzyme catalyzing the degradation of lysoplasmalogen.
CC Lysoplasmalogens are formed by the hydrolysis of the abundant membrane
CC glycerophospholipids plasmalogens. May control the respective levels of
CC plasmalogens and lysoplasmalogens in cells and modulate cell membrane
CC properties. {ECO:0000269|PubMed:21515882}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-
CC saturated aldehyde + sn-glycerol 3-phosphocholine;
CC Xref=Rhea:RHEA:22544, ChEBI:CHEBI:15377, ChEBI:CHEBI:16870,
CC ChEBI:CHEBI:73359, ChEBI:CHEBI:77287; EC=3.3.2.2;
CC Evidence={ECO:0000269|PubMed:21515882};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a
CC 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine;
CC Xref=Rhea:RHEA:16905, ChEBI:CHEBI:15377, ChEBI:CHEBI:73359,
CC ChEBI:CHEBI:77288, ChEBI:CHEBI:143890; EC=3.3.2.2;
CC Evidence={ECO:0000269|PubMed:21515882};
CC -!- ACTIVITY REGULATION: Competitively inhibited by lysophosphatidic acid.
CC {ECO:0000269|PubMed:21515882}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=45 uM for lysoplasmenyl-choline {ECO:0000269|PubMed:21515882};
CC KM=53 uM for lysoplasmenyl-ethanolamine
CC {ECO:0000269|PubMed:21515882};
CC pH dependence:
CC Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and
CC lysoplasmenyl-choline as substrates, respectively.
CC {ECO:0000269|PubMed:21515882};
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q8N661; Q13520: AQP6; NbExp=3; IntAct=EBI-2548832, EBI-13059134;
CC Q8N661; Q3SXY8: ARL13B; NbExp=3; IntAct=EBI-2548832, EBI-11343438;
CC Q8N661; Q8N6S5: ARL6IP6; NbExp=3; IntAct=EBI-2548832, EBI-2808844;
CC Q8N661; P07307-3: ASGR2; NbExp=3; IntAct=EBI-2548832, EBI-12808270;
CC Q8N661; Q9BXK5: BCL2L13; NbExp=6; IntAct=EBI-2548832, EBI-747430;
CC Q8N661; Q13323: BIK; NbExp=3; IntAct=EBI-2548832, EBI-700794;
CC Q8N661; O95393: BMP10; NbExp=3; IntAct=EBI-2548832, EBI-3922513;
CC Q8N661; O14523: C2CD2L; NbExp=3; IntAct=EBI-2548832, EBI-12822627;
CC Q8N661; Q9P0B6: CCDC167; NbExp=3; IntAct=EBI-2548832, EBI-9083477;
CC Q8N661; Q96HJ3-2: CCDC34; NbExp=3; IntAct=EBI-2548832, EBI-17641690;
CC Q8N661; P13236: CCL4; NbExp=3; IntAct=EBI-2548832, EBI-2873970;
CC Q8N661; P19397: CD53; NbExp=3; IntAct=EBI-2548832, EBI-6657396;
CC Q8N661; P11912: CD79A; NbExp=3; IntAct=EBI-2548832, EBI-7797864;
CC Q8N661; P58418: CLRN1; NbExp=3; IntAct=EBI-2548832, EBI-17274839;
CC Q8N661; O43169: CYB5B; NbExp=3; IntAct=EBI-2548832, EBI-1058710;
CC Q8N661; Q8N5I4: DHRSX; NbExp=3; IntAct=EBI-2548832, EBI-3923585;
CC Q8N661; Q96LL9: DNAJC30; NbExp=3; IntAct=EBI-2548832, EBI-8639143;
CC Q8N661; Q15125: EBP; NbExp=3; IntAct=EBI-2548832, EBI-3915253;
CC Q8N661; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-2548832, EBI-781551;
CC Q8N661; P30040: ERP29; NbExp=3; IntAct=EBI-2548832, EBI-946830;
CC Q8N661; P34910-2: EVI2B; NbExp=3; IntAct=EBI-2548832, EBI-17640610;
CC Q8N661; Q9Y624: F11R; NbExp=3; IntAct=EBI-2548832, EBI-742600;
CC Q8N661; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-2548832, EBI-18304435;
CC Q8N661; Q96IV6: FAXDC2; NbExp=3; IntAct=EBI-2548832, EBI-12142299;
CC Q8N661; Q9Y680: FKBP7; NbExp=3; IntAct=EBI-2548832, EBI-3918971;
CC Q8N661; Q9H0Q3: FXYD6; NbExp=3; IntAct=EBI-2548832, EBI-713304;
CC Q8N661; P17302: GJA1; NbExp=3; IntAct=EBI-2548832, EBI-1103439;
CC Q8N661; P35212: GJA4; NbExp=3; IntAct=EBI-2548832, EBI-6918707;
CC Q8N661; P48165: GJA8; NbExp=3; IntAct=EBI-2548832, EBI-17458373;
CC Q8N661; Q8NBJ4: GOLM1; NbExp=3; IntAct=EBI-2548832, EBI-712073;
CC Q8N661; O60883: GPR37L1; NbExp=3; IntAct=EBI-2548832, EBI-2927498;
CC Q8N661; Q8TED1: GPX8; NbExp=3; IntAct=EBI-2548832, EBI-11721746;
CC Q8N661; Q02747: GUCA2A; NbExp=3; IntAct=EBI-2548832, EBI-12244272;
CC Q8N661; P31937: HIBADH; NbExp=3; IntAct=EBI-2548832, EBI-11427100;
CC Q8N661; Q7Z5P4: HSD17B13; NbExp=3; IntAct=EBI-2548832, EBI-18053395;
CC Q8N661; Q9Y5U9: IER3IP1; NbExp=3; IntAct=EBI-2548832, EBI-725665;
CC Q8N661; Q01628: IFITM3; NbExp=3; IntAct=EBI-2548832, EBI-7932862;
CC Q8N661; P43628: KIR2DL3; NbExp=3; IntAct=EBI-2548832, EBI-8632435;
CC Q8N661; Q86VI4: LAPTM4B; NbExp=3; IntAct=EBI-2548832, EBI-3267258;
CC Q8N661; Q9H400: LIME1; NbExp=3; IntAct=EBI-2548832, EBI-2830566;
CC Q8N661; P48449: LSS; NbExp=3; IntAct=EBI-2548832, EBI-3930711;
CC Q8N661; Q14210: LY6D; NbExp=3; IntAct=EBI-2548832, EBI-1965225;
CC Q8N661; Q9P0N8: MARCHF2; NbExp=3; IntAct=EBI-2548832, EBI-10317612;
CC Q8N661; Q9GZY8-5: MFF; NbExp=6; IntAct=EBI-2548832, EBI-11956541;
CC Q8N661; O14880: MGST3; NbExp=3; IntAct=EBI-2548832, EBI-724754;
CC Q8N661; Q8N4V1: MMGT1; NbExp=3; IntAct=EBI-2548832, EBI-6163737;
CC Q8N661; Q96JA4: MS4A14; NbExp=3; IntAct=EBI-2548832, EBI-12839612;
CC Q8N661; Q15800: MSMO1; NbExp=3; IntAct=EBI-2548832, EBI-949102;
CC Q8N661; P15941-11: MUC1; NbExp=3; IntAct=EBI-2548832, EBI-17263240;
CC Q8N661; Q9Y375: NDUFAF1; NbExp=3; IntAct=EBI-2548832, EBI-741874;
CC Q8N661; O14524-2: NEMP1; NbExp=3; IntAct=EBI-2548832, EBI-10969203;
CC Q8N661; Q9NZG7: NINJ2; NbExp=3; IntAct=EBI-2548832, EBI-10317425;
CC Q8N661; Q53FV1: ORMDL2; NbExp=3; IntAct=EBI-2548832, EBI-11075081;
CC Q8N661; Q9NUU6: OTULINL; NbExp=3; IntAct=EBI-2548832, EBI-6916492;
CC Q8N661; Q96HA9: PEX11G; NbExp=3; IntAct=EBI-2548832, EBI-17284886;
CC Q8N661; O15173: PGRMC2; NbExp=3; IntAct=EBI-2548832, EBI-1050125;
CC Q8N661; Q07326: PIGF; NbExp=3; IntAct=EBI-2548832, EBI-17180304;
CC Q8N661; P26678: PLN; NbExp=3; IntAct=EBI-2548832, EBI-692836;
CC Q8N661; P18031: PTPN1; NbExp=3; IntAct=EBI-2548832, EBI-968788;
CC Q8N661; P43378: PTPN9; NbExp=3; IntAct=EBI-2548832, EBI-742898;
CC Q8N661; Q8TEB9: RHBDD1; NbExp=3; IntAct=EBI-2548832, EBI-9916444;
CC Q8N661; Q8IXI1: RHOT2; NbExp=3; IntAct=EBI-2548832, EBI-1396563;
CC Q8N661; Q9NS64: RPRM; NbExp=3; IntAct=EBI-2548832, EBI-1052363;
CC Q8N661; Q5QGT7: RTP2; NbExp=3; IntAct=EBI-2548832, EBI-10244780;
CC Q8N661; O75396: SEC22B; NbExp=3; IntAct=EBI-2548832, EBI-1058865;
CC Q8N661; Q8N474: SFRP1; NbExp=3; IntAct=EBI-2548832, EBI-3940687;
CC Q8N661; Q8WV19: SFT2D1; NbExp=3; IntAct=EBI-2548832, EBI-2854842;
CC Q8N661; Q8TD22: SFXN5; NbExp=3; IntAct=EBI-2548832, EBI-17274136;
CC Q8N661; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-2548832, EBI-18159983;
CC Q8N661; Q8TBE7: SLC35G2; NbExp=3; IntAct=EBI-2548832, EBI-727304;
CC Q8N661; Q9NUM3: SLC39A9; NbExp=3; IntAct=EBI-2548832, EBI-2823239;
CC Q8N661; B2RUZ4: SMIM1; NbExp=3; IntAct=EBI-2548832, EBI-12188413;
CC Q8N661; Q6UX34: SNORC; NbExp=3; IntAct=EBI-2548832, EBI-11957067;
CC Q8N661; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-2548832, EBI-17280858;
CC Q8N661; P27105: STOM; NbExp=3; IntAct=EBI-2548832, EBI-1211440;
CC Q8N661; Q86Y82: STX12; NbExp=3; IntAct=EBI-2548832, EBI-2691717;
CC Q8N661; O43752: STX6; NbExp=3; IntAct=EBI-2548832, EBI-2695795;
CC Q8N661; Q9UNK0: STX8; NbExp=3; IntAct=EBI-2548832, EBI-727240;
CC Q8N661; Q8N6K0: TEX29; NbExp=3; IntAct=EBI-2548832, EBI-19027521;
CC Q8N661; Q8WY91: THAP4; NbExp=3; IntAct=EBI-2548832, EBI-726691;
CC Q8N661; P07204: THBD; NbExp=3; IntAct=EBI-2548832, EBI-941422;
CC Q8N661; O14925: TIMM23; NbExp=3; IntAct=EBI-2548832, EBI-1047996;
CC Q8N661; Q96A25: TMEM106A; NbExp=3; IntAct=EBI-2548832, EBI-3915978;
CC Q8N661; Q6UX40: TMEM107; NbExp=3; IntAct=EBI-2548832, EBI-12845616;
CC Q8N661; Q9BXJ8: TMEM120A; NbExp=3; IntAct=EBI-2548832, EBI-727322;
CC Q8N661; Q9Y6G1: TMEM14A; NbExp=3; IntAct=EBI-2548832, EBI-2800360;
CC Q8N661; Q9P0S9: TMEM14C; NbExp=3; IntAct=EBI-2548832, EBI-2339195;
CC Q8N661; Q969S6: TMEM203; NbExp=3; IntAct=EBI-2548832, EBI-12274070;
CC Q8N661; Q96Q45-2: TMEM237; NbExp=5; IntAct=EBI-2548832, EBI-10982110;
CC Q8N661; Q9NWD8: TMEM248; NbExp=3; IntAct=EBI-2548832, EBI-10314986;
CC Q8N661; Q4KMG9: TMEM52B; NbExp=3; IntAct=EBI-2548832, EBI-18178701;
CC Q8N661; Q9H2L4: TMEM60; NbExp=3; IntAct=EBI-2548832, EBI-2852148;
CC Q8N661; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-2548832, EBI-2548832;
CC Q8N661; O15393-2: TMPRSS2; NbExp=3; IntAct=EBI-2548832, EBI-12345267;
CC Q8N661; Q9Y320: TMX2; NbExp=3; IntAct=EBI-2548832, EBI-6447886;
CC Q8N661; Q9NSU2-1: TREX1; NbExp=3; IntAct=EBI-2548832, EBI-16746122;
CC Q8N661; A0AVG3: TSNARE1; NbExp=3; IntAct=EBI-2548832, EBI-12003468;
CC Q8N661; Q9NZ43: USE1; NbExp=3; IntAct=EBI-2548832, EBI-742842;
CC Q8N661; P63027: VAMP2; NbExp=3; IntAct=EBI-2548832, EBI-520113;
CC Q8N661; Q15836: VAMP3; NbExp=3; IntAct=EBI-2548832, EBI-722343;
CC Q8N661; O95183: VAMP5; NbExp=3; IntAct=EBI-2548832, EBI-10191195;
CC Q8N661; Q9P0L0: VAPA; NbExp=3; IntAct=EBI-2548832, EBI-1059156;
CC Q8N661; Q8N966: ZDHHC22; NbExp=3; IntAct=EBI-2548832, EBI-10268111;
CC Q8N661; Q96FB2; NbExp=3; IntAct=EBI-2548832, EBI-2857623;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cytoplasm {ECO:0000269|PubMed:21515882}.
CC -!- SIMILARITY: Belongs to the TMEM86 family. {ECO:0000305}.
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DR EMBL; AC010327; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023000; AAH23000.1; -; mRNA.
DR CCDS; CCDS12920.1; -.
DR RefSeq; NP_776165.3; NM_173804.4.
DR AlphaFoldDB; Q8N661; -.
DR BioGRID; 129071; 106.
DR IntAct; Q8N661; 104.
DR STRING; 9606.ENSP00000321038; -.
DR SwissLipids; SLP:000000632; -.
DR PhosphoSitePlus; Q8N661; -.
DR BioMuta; TMEM86B; -.
DR DMDM; 296452936; -.
DR MassIVE; Q8N661; -.
DR PaxDb; Q8N661; -.
DR PeptideAtlas; Q8N661; -.
DR PRIDE; Q8N661; -.
DR ProteomicsDB; 72134; -.
DR Antibodypedia; 33044; 13 antibodies from 7 providers.
DR DNASU; 255043; -.
DR Ensembl; ENST00000327042.5; ENSP00000321038.3; ENSG00000180089.6.
DR GeneID; 255043; -.
DR KEGG; hsa:255043; -.
DR MANE-Select; ENST00000327042.5; ENSP00000321038.3; NM_173804.5; NP_776165.3.
DR UCSC; uc002qju.4; human.
DR CTD; 255043; -.
DR DisGeNET; 255043; -.
DR GeneCards; TMEM86B; -.
DR HGNC; HGNC:28448; TMEM86B.
DR HPA; ENSG00000180089; Tissue enhanced (gallbladder, liver).
DR MIM; 617806; gene.
DR neXtProt; NX_Q8N661; -.
DR OpenTargets; ENSG00000180089; -.
DR PharmGKB; PA142670738; -.
DR VEuPathDB; HostDB:ENSG00000180089; -.
DR eggNOG; KOG4804; Eukaryota.
DR GeneTree; ENSGT00390000007101; -.
DR HOGENOM; CLU_079086_1_0_1; -.
DR InParanoid; Q8N661; -.
DR OMA; AWDTFAQ; -.
DR OrthoDB; 1516092at2759; -.
DR PhylomeDB; Q8N661; -.
DR TreeFam; TF324663; -.
DR BioCyc; MetaCyc:ENSG00000180089-MON; -.
DR PathwayCommons; Q8N661; -.
DR Reactome; R-HSA-1482788; Acyl chain remodelling of PC.
DR SignaLink; Q8N661; -.
DR BioGRID-ORCS; 255043; 10 hits in 1082 CRISPR screens.
DR GenomeRNAi; 255043; -.
DR Pharos; Q8N661; Tbio.
DR PRO; PR:Q8N661; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q8N661; protein.
DR Bgee; ENSG00000180089; Expressed in right lobe of liver and 89 other tissues.
DR Genevisible; Q8N661; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0047408; F:alkenylglycerophosphocholine hydrolase activity; IDA:UniProtKB.
DR GO; GO:0047409; F:alkenylglycerophosphoethanolamine hydrolase activity; IDA:UniProtKB.
DR GO; GO:0016803; F:ether hydrolase activity; TAS:Reactome.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046485; P:ether lipid metabolic process; IDA:UniProtKB.
DR GO; GO:0036151; P:phosphatidylcholine acyl-chain remodeling; TAS:Reactome.
DR InterPro; IPR012506; YhhN.
DR PANTHER; PTHR31885; PTHR31885; 1.
DR Pfam; PF07947; YhhN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Lipid metabolism; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..226
FT /note="Lysoplasmalogenase"
FT /id="PRO_0000201841"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 176
FT /note="A -> T (in dbSNP:rs35608872)"
FT /id="VAR_052343"
FT VARIANT 199
FT /note="H -> R (in dbSNP:rs4644955)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_052344"
SQ SEQUENCE 226 AA; 24352 MW; BD5344268F221275 CRC64;
MDAGKAGQTL KTHCSAQRPD VCRWLSPFIL SCCVYFCLWI PEDQLSWFAA LVKCLPVLCL
AGFLWVMSPS GGYTQLLQGA LVCSAVGDAC LIWPAAFVPG MAAFATAHLL YVWAFGFSPL
QPGLLLLIIL APGPYLSLVL QHLEPDMVLP VAAYGLILMA MLWRGLAQGG SAGWGALLFT
LSDGVLAWDT FAQPLPHAHL VIMTTYYAAQ LLITLSALRS PVPKTD
