TM87A_MOUSE
ID TM87A_MOUSE Reviewed; 555 AA.
AC Q8BXN9; Q8BTV9; Q8BU98; Q8R2P9; Q8R3S7;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Transmembrane protein 87A {ECO:0000305};
DE Flags: Precursor;
GN Name=Tmem87a {ECO:0000312|MGI:MGI:2441844};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Head, Retina, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 328-555 (ISOFORM 1).
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-540, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-157 AND ASN-160.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-540, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in retrograde transport from endosomes to the
CC trans-Golgi network (TGN). {ECO:0000250|UniProtKB:Q8NBN3}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q8NBN3}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BXN9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BXN9-2; Sequence=VSP_022210;
CC Name=3;
CC IsoId=Q8BXN9-3; Sequence=VSP_022210, VSP_022211, VSP_022212;
CC -!- SIMILARITY: Belongs to the LU7TM family. TMEM87 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH24676.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC39731.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC40424.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK044591; BAC31991.1; -; mRNA.
DR EMBL; AK086738; BAC39731.1; ALT_INIT; mRNA.
DR EMBL; AK088559; BAC40424.1; ALT_INIT; mRNA.
DR EMBL; BC024676; AAH24676.1; ALT_INIT; mRNA.
DR EMBL; BC027354; AAH27354.1; -; mRNA.
DR RefSeq; NP_001103966.1; NM_001110496.1.
DR RefSeq; NP_001103967.1; NM_001110497.1.
DR RefSeq; NP_776095.2; NM_173734.3.
DR AlphaFoldDB; Q8BXN9; -.
DR STRING; 10090.ENSMUSP00000087500; -.
DR GlyConnect; 2797; 1 N-Linked glycan (1 site).
DR GlyGen; Q8BXN9; 4 sites, 1 N-linked glycan (1 site).
DR iPTMnet; Q8BXN9; -.
DR PhosphoSitePlus; Q8BXN9; -.
DR SwissPalm; Q8BXN9; -.
DR EPD; Q8BXN9; -.
DR MaxQB; Q8BXN9; -.
DR PaxDb; Q8BXN9; -.
DR PeptideAtlas; Q8BXN9; -.
DR PRIDE; Q8BXN9; -.
DR ProteomicsDB; 259117; -. [Q8BXN9-1]
DR ProteomicsDB; 259118; -. [Q8BXN9-2]
DR ProteomicsDB; 259119; -. [Q8BXN9-3]
DR GeneID; 211499; -.
DR KEGG; mmu:211499; -.
DR CTD; 25963; -.
DR MGI; MGI:2441844; Tmem87a.
DR eggNOG; KOG2568; Eukaryota.
DR InParanoid; Q8BXN9; -.
DR OrthoDB; 1448608at2759; -.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR BioGRID-ORCS; 211499; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Tmem87a; mouse.
DR PRO; PR:Q8BXN9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8BXN9; protein.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0032580; C:Golgi cisterna membrane; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISO:MGI.
DR InterPro; IPR009637; GPR107/GPR108-like.
DR PANTHER; PTHR21229; PTHR21229; 1.
DR Pfam; PF06814; Lung_7-TM_R; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..555
FT /note="Transmembrane protein 87A"
FT /id="PRO_0000270752"
FT TOPO_DOM 22..225
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 226..246
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 258..278
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..305
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 306..322
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 326..346
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..361
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 362..382
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 404..424
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425..437
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 438..458
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..555
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 491..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 540
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 160
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT VAR_SEQ 355..376
FT /note="AQTDLASLAFIPLAFLDTALCW -> YFSYSLALIVSLALSAIDACIIL
FT (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_022210"
FT VAR_SEQ 469..483
FT /note="FAFSPLSEEDEEDEQ -> RNENEKYQAGTKWD (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022211"
FT VAR_SEQ 484..555
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022212"
FT CONFLICT 116
FT /note="G -> W (in Ref. 1; BAC40424)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="F -> L (in Ref. 1; BAC40424)"
FT /evidence="ECO:0000305"
FT CONFLICT 491
FT /note="S -> G (in Ref. 1; BAC39731)"
FT /evidence="ECO:0000305"
FT CONFLICT 514
FT /note="Missing (in Ref. 1; BAC39731)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 555 AA; 63380 MW; 4BC075BF0491A826 CRC64;
MAVAAWLQVS PVIFLLLGAQ PFPLSFLGAG PAPVFAADRS KWHIPMPSGK GYFNFGKILF
RNTTILLKFD GEPCDQSLNI TWFLKSADCY NEIYNFKADE IESYLENLKG KKGLSGRYQT
SSRLFQNCSE LYKAQSFSGD FTHRLPLLGE KQEAKENATN VTFTGDKIAM HEPLQTWQDA
PYIFIVHVGI SSSKESPKEN ALSNLFTMTV EVKGPYEYLT LEDYPLMIFF MVMCIVYVLF
GVLWLAWSAC YWRDLLRIQF WIGAVIFLGM FEKAVFYAEF QNIRYKGESV QNALVLAELL
SAVKRSLART LVIIVSLGYG IVKPRLGVTL HKVVVAGALY LLFSGMEGVL RVTGAQTDLA
SLAFIPLAFL DTALCWWIFI SLTQTMKLLK LRRNIVKLSL YRHFTNTLIL AVAASIVFII
WTTMKFRIVT CQSDWRELWV DDAIWRLLFS MILFVIMILW RPSANNQRFA FSPLSEEDEE
DEQKEPMLKE SFEGMKMRST KQEPNGTSKV NKAQEDDLKW VEENVPSSVT DVALPALLDS
DEERMITHFE RSKME
