TM9S3_HUMAN
ID TM9S3_HUMAN Reviewed; 589 AA.
AC Q9HD45; Q5TB57; Q6UWE7; Q9NWL8; Q9P0G9; Q9UHW8;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Transmembrane 9 superfamily member 3;
DE AltName: Full=EP70-P-iso;
DE AltName: Full=SM-11044-binding protein;
DE Flags: Precursor;
GN Name=TM9SF3; Synonyms=SMBP; ORFNames=UNQ245/PRO282;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Warner S.J., Lomax M.I.;
RT "Evolution of the TM9 super family of membrane spanning proteins.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Adrenal gland;
RA Gu Y., Peng Y., Li Y., Fu S., Gu J., Gu W., Jiang C., Yu Y., Han Z.,
RA Wang Y., Chen Z., Fu G.;
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 12-589.
RC TISSUE=Skeletal muscle;
RA Sugasawa T., Lenzen G., Simon S., Hidaka J., Cahen A., Guillaume J.L.,
RA Camoin L., Nahmias C., Strosberg A.D.;
RT "The iodocyanopindolol and SM-11044 binding protein (SMBP) belongs to the
RT emerging family of MP70 multispanning membrane proteins.";
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-589.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 128-589.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [9]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-174.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [10]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-174.
RC TISSUE=Leukemic T-cell;
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF67014.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAQ89178.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAA91362.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF269150; AAF98159.1; -; mRNA.
DR EMBL; AF160213; AAF67014.1; ALT_FRAME; mRNA.
DR EMBL; AL138765; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471066; EAW49972.1; -; Genomic_DNA.
DR EMBL; CH471066; EAW49974.1; -; Genomic_DNA.
DR EMBL; BC136788; AAI36789.1; -; mRNA.
DR EMBL; BC136789; AAI36790.1; -; mRNA.
DR EMBL; AF116347; AAF21983.1; -; mRNA.
DR EMBL; AY358819; AAQ89178.1; ALT_FRAME; mRNA.
DR EMBL; AK000756; BAA91362.1; ALT_INIT; mRNA.
DR CCDS; CCDS7450.1; -.
DR RefSeq; NP_064508.3; NM_020123.3.
DR AlphaFoldDB; Q9HD45; -.
DR SMR; Q9HD45; -.
DR BioGRID; 121219; 140.
DR IntAct; Q9HD45; 31.
DR MINT; Q9HD45; -.
DR STRING; 9606.ENSP00000360184; -.
DR GlyConnect; 1842; 33 N-Linked glycans (1 site).
DR GlyGen; Q9HD45; 3 sites, 32 N-linked glycans (1 site), 1 O-linked glycan (1 site).
DR iPTMnet; Q9HD45; -.
DR PhosphoSitePlus; Q9HD45; -.
DR SwissPalm; Q9HD45; -.
DR BioMuta; TM9SF3; -.
DR DMDM; 13878808; -.
DR EPD; Q9HD45; -.
DR jPOST; Q9HD45; -.
DR MassIVE; Q9HD45; -.
DR MaxQB; Q9HD45; -.
DR PaxDb; Q9HD45; -.
DR PeptideAtlas; Q9HD45; -.
DR PRIDE; Q9HD45; -.
DR ProteomicsDB; 81832; -.
DR Antibodypedia; 50246; 89 antibodies from 20 providers.
DR DNASU; 56889; -.
DR Ensembl; ENST00000371142.9; ENSP00000360184.4; ENSG00000077147.16.
DR GeneID; 56889; -.
DR KEGG; hsa:56889; -.
DR MANE-Select; ENST00000371142.9; ENSP00000360184.4; NM_020123.4; NP_064508.3.
DR UCSC; uc001kmm.5; human.
DR CTD; 56889; -.
DR DisGeNET; 56889; -.
DR GeneCards; TM9SF3; -.
DR HGNC; HGNC:21529; TM9SF3.
DR HPA; ENSG00000077147; Low tissue specificity.
DR neXtProt; NX_Q9HD45; -.
DR OpenTargets; ENSG00000077147; -.
DR PharmGKB; PA144596264; -.
DR VEuPathDB; HostDB:ENSG00000077147; -.
DR eggNOG; KOG1277; Eukaryota.
DR GeneTree; ENSGT00940000155493; -.
DR HOGENOM; CLU_010714_0_2_1; -.
DR InParanoid; Q9HD45; -.
DR OMA; APCRVNA; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q9HD45; -.
DR TreeFam; TF314271; -.
DR PathwayCommons; Q9HD45; -.
DR SignaLink; Q9HD45; -.
DR BioGRID-ORCS; 56889; 79 hits in 1086 CRISPR screens.
DR ChiTaRS; TM9SF3; human.
DR GenomeRNAi; 56889; -.
DR Pharos; Q9HD45; Tbio.
DR PRO; PR:Q9HD45; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q9HD45; protein.
DR Bgee; ENSG00000077147; Expressed in calcaneal tendon and 202 other tissues.
DR ExpressionAtlas; Q9HD45; baseline and differential.
DR Genevisible; Q9HD45; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..589
FT /note="Transmembrane 9 superfamily member 3"
FT /id="PRO_0000034369"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 519..539
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 551..571
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218,
FT ECO:0000269|PubMed:19349973"
FT CARBOHYD 419
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 15
FT /note="Missing (in Ref. 2; AAF67014)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="M -> I (in Ref. 1; AAF98159)"
FT /evidence="ECO:0000305"
FT CONFLICT 210..218
FT /note="KYLDPSFFQ -> NILIVLFS (in Ref. 2; AAF67014)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="M -> T (in Ref. 8; BAA91362)"
FT /evidence="ECO:0000305"
FT CONFLICT 398
FT /note="V -> R (in Ref. 7; AAQ89178)"
FT /evidence="ECO:0000305"
FT CONFLICT 547
FT /note="F -> S (in Ref. 8; BAA91362)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 589 AA; 67888 MW; E673D547A35BC4A7 CRC64;
MRPLPGALGV AAAAALWLLL LLLPRTRADE HEHTYQDKEE VVLWMNTVGP YHNRQETYKY
FSLPFCVGSK KSISHYHETL GEALQGVELE FSGLDIKFKD DVMPATYCEI DLDKEKRDAF
VYAIKNHYWY QMYIDDLPIW GIVGEADENG EDYYLWTYKK LEIGFNGNRI VDVNLTSEGK
VKLVPNTKIQ MSYSVKWKKS DVKFEDRFDK YLDPSFFQHR IHWFSIFNSF MMVIFLVGLV
SMILMRTLRK DYARYSKEEE MDDMDRDLGD EYGWKQVHGD VFRPSSHPLI FSSLIGSGCQ
IFAVSLIVII VAMIEDLYTE RGSMLSTAIF VYAATSPVNG YFGGSLYARQ GGRRWIKQMF
IGAFLIPAMV CGTAFFINFI AIYYHASRAI PFGTMVAVCC ICFFVILPLN LVGTILGRNL
SGQPNFPCRV NAVPRPIPEK KWFMEPAVIV CLGGILPFGS IFIEMYFIFT SFWAYKIYYV
YGFMMLVLVI LCIVTVCVTI VCTYFLLNAE DYRWQWTSFL SAASTAIYVY MYSFYYYFFK
TKMYGLFQTS FYFGYMAVFS TALGIMCGAI GYMGTSAFVR KIYTNVKID
