TM9S4_MOUSE
ID TM9S4_MOUSE Reviewed; 643 AA.
AC Q8BH24; Q2TBF8; Q3TB27; Q8BU80; Q8BXB4; Q8CHH4;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Transmembrane 9 superfamily member 4;
DE Flags: Precursor;
GN Name=Tm9sf4; Synonyms=Kiaa0255;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic tail;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Head, Lung, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Liver, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Associates with proteins harboring glycine-rich transmembrane
CC domains and ensures their efficient localization to the cell surface.
CC {ECO:0000250|UniProtKB:Q92544}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Golgi apparatus {ECO:0000250|UniProtKB:Q92544}.
CC Early endosome {ECO:0000250|UniProtKB:Q92544}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC {ECO:0000305}.
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DR EMBL; AB093220; BAC41404.3; -; mRNA.
DR EMBL; AK048158; BAC33261.1; -; mRNA.
DR EMBL; AK083541; BAC38948.1; -; mRNA.
DR EMBL; AK087048; BAC39789.1; -; mRNA.
DR EMBL; AK088012; BAC40094.1; -; mRNA.
DR EMBL; AK162435; BAE36916.1; -; mRNA.
DR EMBL; AK171487; BAE42487.1; -; mRNA.
DR EMBL; AL807380; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC110309; AAI10310.1; -; mRNA.
DR CCDS; CCDS16906.1; -.
DR RefSeq; NP_598608.2; NM_133847.3.
DR AlphaFoldDB; Q8BH24; -.
DR SMR; Q8BH24; -.
DR STRING; 10090.ENSMUSP00000086422; -.
DR PhosphoSitePlus; Q8BH24; -.
DR SwissPalm; Q8BH24; -.
DR EPD; Q8BH24; -.
DR jPOST; Q8BH24; -.
DR MaxQB; Q8BH24; -.
DR PaxDb; Q8BH24; -.
DR PeptideAtlas; Q8BH24; -.
DR PRIDE; Q8BH24; -.
DR ProteomicsDB; 262834; -.
DR Antibodypedia; 42955; 120 antibodies from 20 providers.
DR DNASU; 99237; -.
DR Ensembl; ENSMUST00000089027; ENSMUSP00000086422; ENSMUSG00000068040.
DR GeneID; 99237; -.
DR KEGG; mmu:99237; -.
DR UCSC; uc008nhd.1; mouse.
DR CTD; 9777; -.
DR MGI; MGI:2139220; Tm9sf4.
DR VEuPathDB; HostDB:ENSMUSG00000068040; -.
DR eggNOG; KOG1278; Eukaryota.
DR GeneTree; ENSGT00940000157198; -.
DR HOGENOM; CLU_010714_4_1_1; -.
DR InParanoid; Q8BH24; -.
DR OMA; CYPKNGT; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q8BH24; -.
DR TreeFam; TF354239; -.
DR BioGRID-ORCS; 99237; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Tm9sf4; mouse.
DR PRO; PR:Q8BH24; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BH24; protein.
DR Bgee; ENSMUSG00000068040; Expressed in humerus cartilage element and 252 other tissues.
DR Genevisible; Q8BH24; MM.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; ISO:MGI.
DR GO; GO:0006909; P:phagocytosis; ISO:MGI.
DR GO; GO:0070863; P:positive regulation of protein exit from endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:2000010; P:positive regulation of protein localization to cell surface; ISS:UniProtKB.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; ISO:MGI.
DR GO; GO:0001666; P:response to hypoxia; ISO:MGI.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; ISO:MGI.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..643
FT /note="Transmembrane 9 superfamily member 4"
FT /id="PRO_0000311810"
FT TOPO_DOM 24..282
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439..450
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..502
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 503..523
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 524..536
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 537..557
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 558..571
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 572..592
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 593..599
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 600..620
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 621..643
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 313
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q92544"
FT CONFLICT 63
FT /note="F -> S (in Ref. 4; AAI10310)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="I -> V (in Ref. 2; BAC39789)"
FT /evidence="ECO:0000305"
FT CONFLICT 485
FT /note="N -> K (in Ref. 2; BAC33261)"
FT /evidence="ECO:0000305"
FT CONFLICT 589
FT /note="F -> S (in Ref. 2; BAE42487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 643 AA; 74693 MW; 560EA3F4E3376B17 CRC64;
MAAAMIWWPR FLLLLCLTCK GSTFYVPGVA PINFHQNDPV EIKAVKLTSS RTQLPYEYYS
LPFCQPIKIT YKAENLGEVL RGDRIVNTPF QVLMNSEKKC EVLCNQSNKP ITLTVEQSRL
VAERITEEYY VHLIADNLPV ATRLELYSSN RDSDDKKKEK DVQFEHGYRL GFTDVNKIYL
HNHLSFILYY HREDMEEDQE HTYRVVRFEV IPQSIRLEDL KTGEKSSCTL PEGANSLPQE
IDPTKENQLY FTYSVHWEES DIKWASRWDT YLTMSDVQIH WFSIINSVVV VFFLSGILSM
IIIRTLRKDI ANYNKEDDIE DTMEESGWKL VHGDVFRPPQ YPMILSSLLG SGIQLFCMIL
IVIFVAMLGM LSPSSRGALM TTACFLFMFM GVFGGFSAGR LYRTLKGHRW KKGAFCTATL
YPGVVFGICF VLNCFIWGKH SSGAVPFPTM VALLCMWFGI SLPLVYLGYY FGFRKQPYDN
PVRTNQIPRQ IPEQRWYMNR FVGILMAGIL PFGAMFIELF FIFSAIWENQ FYYLFGFLFL
VFIILVVSCS QISIVMVYFQ LCAEDYRWWW RNFLVSGGSA FYVLVYAIFY FVNKLDIVEF
IPSLLYFGYT TLMVLSFWLL TGTIGFYAAY MFVRKIYAAV KID
