ID   TMA20_ASHGO             Reviewed;         181 AA.
AC   Q75AA8;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Translation machinery-associated protein 20;
GN   Name=TMA20; OrderedLocusNames=ADR010C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Involved in translation. {ECO:0000250}.
CC   -!- SUBUNIT: Associates with ribosomal complexes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TMA20 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS51930.1; -; Genomic_DNA.
DR   RefSeq; NP_984106.1; NM_209459.1.
DR   AlphaFoldDB; Q75AA8; -.
DR   SMR; Q75AA8; -.
DR   STRING; 33169.AAS51930; -.
DR   EnsemblFungi; AAS51930; AAS51930; AGOS_ADR010C.
DR   GeneID; 4620254; -.
DR   KEGG; ago:AGOS_ADR010C; -.
DR   eggNOG; KOG2523; Eukaryota.
DR   HOGENOM; CLU_090468_0_1_1; -.
DR   InParanoid; Q75AA8; -.
DR   OMA; MFKKFSP; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001731; P:formation of translation preinitiation complex; IBA:GO_Central.
DR   GO; GO:0042254; P:ribosome biogenesis; IEA:EnsemblFungi.
DR   InterPro; IPR016437; MCT-1/Tma20.
DR   InterPro; IPR041366; Pre-PUA.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR004521; Uncharacterised_CHP00451.
DR   PANTHER; PTHR22798; PTHR22798; 1.
DR   Pfam; PF17832; Pre-PUA; 1.
DR   Pfam; PF01472; PUA; 1.
DR   PIRSF; PIRSF005067; Tma_RNA-bind_prd; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..181
FT                   /note="Translation machinery-associated protein 20"
FT                   /id="PRO_0000245366"
FT   DOMAIN          90..172
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
SQ   SEQUENCE   181 AA;  20224 MW;  230BAB65A2A954C5 CRC64;
     MFKKFSKEDI HTRTKVKTSV QRSLKSKLVE QFPKLEEVID ELLPKKTELQ IIKCSDKIQL
     YAVGDEVLFF QKFEELIPTL RFVHKYPEAF PTIQIDRGAI KFVLSGANIM CPGLTSSGAA
     LPEAPGLAQD SIVVVNAENK ENALAIGKLL MSTEDIKSIN KGHGVETIHH LGDCLWNFSL
     V