ID   TMBL2_THEKO             Reviewed;         264 AA.
AC   Q5JD67;
DT   01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=HTH-type transcriptional regulator TrmBL2 {ECO:0000303|PubMed:21148291};
GN   Name=trmBL2 {ECO:0000303|PubMed:21148291}; OrderedLocusNames=TK0471;
OS   Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS   (Pyrococcus kodakaraensis (strain KOD1)).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=69014;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=15710748; DOI=10.1101/gr.3003105;
RA   Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT   "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT   kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL   Genome Res. 15:352-363(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=21148291; DOI=10.1091/mbc.e10-08-0668;
RA   Maruyama H., Shin M., Oda T., Matsumi R., Ohniwa R.L., Itoh T.,
RA   Shirahige K., Imanaka T., Atomi H., Yoshimura S.H., Takeyasu K.;
RT   "Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture
RT   in the hyperthermophilic archaeon Thermococcus kodakarensis.";
RL   Mol. Biol. Cell 22:386-398(2011).
CC   -!- FUNCTION: An abundant chromosomal protein that seems to be involved in
CC       both genome architecture and transcription repression. Incubation with
CC       DNA in vitro gives fibrous structures 14.2 +/- 2.1 nm in thickness
CC       (naked DNA is 1.83 +/- 0.37 nm); does not significantly compact DNA.
CC       Binds to both coding and non-coding regions; binding within gene
CC       promoters correlates with decreased transcript levels, while binding
CC       within coding regions does not. {ECO:0000269|PubMed:21148291}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:21148291}.
CC       Chromosome {ECO:0000269|PubMed:21148291}.
CC   -!- DISRUPTION PHENOTYPE: Slight growth delay compared to wild-type. No
CC       fibrous chromosomal DNA isolated from cells, DNA condensation is
CC       normal. Chromatin DNA is more sensitive to micrococcal nuclease. Alters
CC       gene expression levels of about 10% of the genome.
CC       {ECO:0000269|PubMed:21148291}.
CC   -!- SIMILARITY: Belongs to the transcriptional regulator TrmB family.
CC       {ECO:0000305}.
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DR   EMBL; AP006878; BAD84660.1; -; Genomic_DNA.
DR   RefSeq; WP_011249426.1; NC_006624.1.
DR   AlphaFoldDB; Q5JD67; -.
DR   SMR; Q5JD67; -.
DR   STRING; 69014.TK0471; -.
DR   EnsemblBacteria; BAD84660; BAD84660; TK0471.
DR   GeneID; 3234163; -.
DR   KEGG; tko:TK0471; -.
DR   PATRIC; fig|69014.16.peg.463; -.
DR   eggNOG; arCOG02037; Archaea.
DR   HOGENOM; CLU_072493_0_2_2; -.
DR   InParanoid; Q5JD67; -.
DR   OMA; YETQIWI; -.
DR   OrthoDB; 72347at2157; -.
DR   PhylomeDB; Q5JD67; -.
DR   Proteomes; UP000000536; Chromosome.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR021586; Tscrpt_reg_TrmB_C.
DR   InterPro; IPR002831; Tscrpt_reg_TrmB_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF11495; Regulator_TrmB; 1.
DR   Pfam; PF01978; TrmB; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Coiled coil; Cytoplasm; DNA-binding; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..264
FT                   /note="HTH-type transcriptional regulator TrmBL2"
FT                   /id="PRO_0000432649"
FT   COILED          81..113
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   264 AA;  30826 MW;  B1053E06F0A35201 CRC64;
     MVKDRMVELL QEHFELNLYE ARAYVALVGF GVLTPAELAS VSEVPAPRTY DVLRSLEKKG
     FAISQPGKVN KYRPVHPENI LEKFIEEWQE RVKEELEAKK KAKEELIELM KPLIETEIPK
     YGVERVWVVR GIRNATLKTK EMFEEVKEKI LLADDGYIAI NLENDLIKAI DNGAKAKIIV
     SKSLLKRLEG SKIMEYAKKG KLELRALDKF ELPMLICDDE VFFALEDMAA RYFNYETQVW
     IKDFRVRDLF EAKFNEYWEK AEKV