TMC4_MOUSE
ID TMC4_MOUSE Reviewed; 694 AA.
AC Q7TQ65; Q7TMJ5; Q7TN62; Q8C7J5; Q8CGE2; Q8R1X9;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Transmembrane channel-like protein 4;
GN Name=Tmc4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=12812529; DOI=10.1186/1471-2164-4-24;
RA Keresztes G., Mutai H., Heller S.;
RT "TMC and EVER genes belong to a larger novel family, the TMC gene family
RT encoding transmembrane proteins.";
RL BMC Genomics 4:24-24(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12906855; DOI=10.1016/s0888-7543(03)00154-x;
RA Kurima K., Yang Y., Sorber K., Griffith A.J.;
RT "Characterization of the transmembrane channel-like (TMC) gene family:
RT functional clues from hearing loss and epidermodysplasia verruciformis.";
RL Genomics 82:300-308(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-694 (ISOFORM 1), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 67-694 (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable ion channel. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7TQ65-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TQ65-2; Sequence=VSP_026015, VSP_026016;
CC Name=3;
CC IsoId=Q7TQ65-3; Sequence=VSP_026014;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:12812529}.
CC -!- SIMILARITY: Belongs to the TMC family. {ECO:0000305}.
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DR EMBL; AY263162; AAP78777.1; -; mRNA.
DR EMBL; AY236493; AAP69871.1; -; mRNA.
DR EMBL; AK050105; BAC34068.1; -; mRNA.
DR EMBL; BC022758; AAH22758.1; -; mRNA.
DR EMBL; BC040466; AAH40466.1; -; mRNA.
DR EMBL; BC055939; AAH55939.1; -; mRNA.
DR CCDS; CCDS20723.1; -. [Q7TQ65-1]
DR RefSeq; NP_861541.2; NM_181820.2.
DR AlphaFoldDB; Q7TQ65; -.
DR STRING; 10090.ENSMUSP00000043853; -.
DR GlyGen; Q7TQ65; 1 site.
DR PhosphoSitePlus; Q7TQ65; -.
DR MaxQB; Q7TQ65; -.
DR PaxDb; Q7TQ65; -.
DR PRIDE; Q7TQ65; -.
DR ProteomicsDB; 259235; -. [Q7TQ65-1]
DR ProteomicsDB; 259236; -. [Q7TQ65-2]
DR ProteomicsDB; 259237; -. [Q7TQ65-3]
DR DNASU; 353499; -.
DR GeneID; 353499; -.
DR KEGG; mmu:353499; -.
DR UCSC; uc009evp.1; mouse. [Q7TQ65-2]
DR CTD; 147798; -.
DR MGI; MGI:2669035; Tmc4.
DR eggNOG; ENOG502S3W0; Eukaryota.
DR InParanoid; Q7TQ65; -.
DR OrthoDB; 1048914at2759; -.
DR PhylomeDB; Q7TQ65; -.
DR BioGRID-ORCS; 353499; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Tmc4; mouse.
DR PRO; PR:Q7TQ65; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q7TQ65; protein.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR InterPro; IPR038900; TMC.
DR InterPro; IPR012496; TMC_dom.
DR PANTHER; PTHR23302; PTHR23302; 1.
DR Pfam; PF07810; TMC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..694
FT /note="Transmembrane channel-like protein 4"
FT /id="PRO_0000289469"
FT TOPO_DOM 1..150
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..330
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 352..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..407
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..513
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 514..534
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..574
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 575..595
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 596..635
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 636..656
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 657..694
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 691
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..67
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026014"
FT VAR_SEQ 415..451
FT /note="TVFLRLASLVFLLVSLWSQITCGGNMEAEGCKACGYN -> FWTHGMGRECR
FT QKGERDRWKKVLQIRQRRAGRGGARL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026015"
FT VAR_SEQ 452..694
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026016"
FT CONFLICT 102
FT /note="R -> T (in Ref. 1; AAP78777)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="S -> A (in Ref. 1; AAP78777)"
FT /evidence="ECO:0000305"
FT CONFLICT 228
FT /note="P -> A (in Ref. 1; AAP78777)"
FT /evidence="ECO:0000305"
FT CONFLICT 245
FT /note="V -> I (in Ref. 3; BAC34068)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="Y -> H (in Ref. 2; AAP69871)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="S -> A (in Ref. 2; AAP69871)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="F -> S (in Ref. 2; AAP69871)"
FT /evidence="ECO:0000305"
FT CONFLICT 441
FT /note="E -> G (in Ref. 2; AAP69871)"
FT /evidence="ECO:0000305"
FT CONFLICT 682
FT /note="Q -> H (in Ref. 3; BAC34068)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 694 AA; 77367 MW; 160D22206E91C83E CRC64;
MEAWGQSPAC SSSRKARTGP SLASVLNDLP SAATLRYRGP GVLPWGTLDE DDDEGGRSLQ
AFAETAQMES HPSRELPWPM QARRAHRKSQ ATGQLASGSE SRAAYWTRQL SRTKGKMKEG
FQTIQPWAWT LKKIGGQFGA GTESYFSLLR FLLFLNLVAS VIEICMKLIP TWLEGAPPGP
PGPNISSPCG SYIPHTHGLV AFPTQLFNLL SGEGYLEWSP LFYGFYPPRS NLAITYLCSV
FAISVIYLLC ILRRSVSGLK ETLLAESDIL TSYSHRVFSA WNFGLCGDVH VRLRQRIILY
ELQVDLEEAV VRRRAAEQTL SQRAKVWSMR ALLNVLVLAL LGAAFYGIYW ATEYTLTLQE
TPLVRQTPLF KLLVDYLPSI FISLFNFVLP PVFKFIASLE GYTQSRQIVL ILLRTVFLRL
ASLVFLLVSL WSQITCGGNM EAEGCKACGY NYKEIPCWET RLGQEMYKLV LFDLLMGLLV
TLLVQFPRKI LCGLCPGALG RLSGTLEFQV PDEVLGLIYA QTVVWVGSFF CPLLPLINTA
KFLILFCLKK ITLFSIYSPA SRTFRASTAN FFFPLVLLVG LAISAVPVLY SIFLIPPSKL
CGPFRGKLSI WAQIPEAIES LPQTAQNFLY FLGTQAFTVP LLILSSILMM YTVALANCYG
RLISELKRQI ETEVQNKVFL AQRAVALSSR NGTS
