TMC5_MOUSE
ID TMC5_MOUSE Reviewed; 967 AA.
AC Q32NZ6; Q7TN61; Q80VR0; Q9D4F1;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Transmembrane channel-like protein 5;
GN Name=Tmc5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=12812529; DOI=10.1186/1471-2164-4-24;
RA Keresztes G., Mutai H., Heller S.;
RT "TMC and EVER genes belong to a larger novel family, the TMC gene family
RT encoding transmembrane proteins.";
RL BMC Genomics 4:24-24(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=12906855; DOI=10.1016/s0888-7543(03)00154-x;
RA Kurima K., Yang Y., Sorber K., Griffith A.J.;
RT "Characterization of the transmembrane channel-like (TMC) gene family:
RT functional clues from hearing loss and epidermodysplasia verruciformis.";
RL Genomics 82:300-308(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable ion channel. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q32NZ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q32NZ6-2; Sequence=VSP_026047, VSP_026048;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:12812529}.
CC -!- SIMILARITY: Belongs to the TMC family. {ECO:0000305}.
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DR EMBL; AY236495; AAP69873.1; -; mRNA.
DR EMBL; AK016573; BAB30314.1; -; mRNA.
DR EMBL; AY263159; AAP78774.1; -; mRNA.
DR EMBL; BC046390; AAH46390.1; -; mRNA.
DR EMBL; BC108382; AAI08383.1; -; mRNA.
DR CCDS; CCDS21773.1; -. [Q32NZ6-2]
DR CCDS; CCDS52374.1; -. [Q32NZ6-1]
DR RefSeq; NP_001098722.1; NM_001105252.1. [Q32NZ6-1]
DR RefSeq; NP_083206.1; NM_028930.3. [Q32NZ6-2]
DR RefSeq; XP_011240226.1; XM_011241924.2. [Q32NZ6-1]
DR AlphaFoldDB; Q32NZ6; -.
DR STRING; 10090.ENSMUSP00000049784; -.
DR iPTMnet; Q32NZ6; -.
DR PhosphoSitePlus; Q32NZ6; -.
DR PaxDb; Q32NZ6; -.
DR PRIDE; Q32NZ6; -.
DR ProteomicsDB; 259240; -. [Q32NZ6-1]
DR ProteomicsDB; 259241; -. [Q32NZ6-2]
DR Antibodypedia; 25361; 78 antibodies from 15 providers.
DR DNASU; 74424; -.
DR Ensembl; ENSMUST00000057320; ENSMUSP00000049784; ENSMUSG00000030650. [Q32NZ6-2]
DR Ensembl; ENSMUST00000098088; ENSMUSP00000095694; ENSMUSG00000030650. [Q32NZ6-1]
DR Ensembl; ENSMUST00000121715; ENSMUSP00000112434; ENSMUSG00000030650. [Q32NZ6-1]
DR Ensembl; ENSMUST00000121744; ENSMUSP00000114137; ENSMUSG00000030650. [Q32NZ6-1]
DR GeneID; 74424; -.
DR KEGG; mmu:74424; -.
DR UCSC; uc009jke.2; mouse. [Q32NZ6-1]
DR UCSC; uc009jkg.2; mouse. [Q32NZ6-2]
DR CTD; 79838; -.
DR MGI; MGI:1921674; Tmc5.
DR VEuPathDB; HostDB:ENSMUSG00000030650; -.
DR eggNOG; ENOG502QQB2; Eukaryota.
DR GeneTree; ENSGT01050000244894; -.
DR HOGENOM; CLU_014592_0_0_1; -.
DR InParanoid; Q32NZ6; -.
DR OMA; FSCWESM; -.
DR OrthoDB; 1048914at2759; -.
DR PhylomeDB; Q32NZ6; -.
DR TreeFam; TF313462; -.
DR BioGRID-ORCS; 74424; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Tmc5; mouse.
DR PRO; PR:Q32NZ6; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q32NZ6; protein.
DR Bgee; ENSMUSG00000030650; Expressed in spermatid and 72 other tissues.
DR ExpressionAtlas; Q32NZ6; baseline and differential.
DR Genevisible; Q32NZ6; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR InterPro; IPR038900; TMC.
DR InterPro; IPR012496; TMC_dom.
DR PANTHER; PTHR23302; PTHR23302; 2.
DR Pfam; PF07810; TMC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion channel; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..967
FT /note="Transmembrane channel-like protein 5"
FT /id="PRO_0000289967"
FT TOPO_DOM 1..420
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..449
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..487
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..581
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 582..602
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 603..616
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 617..637
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 638..660
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 661..681
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 682..694
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 695..715
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 716..749
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 750..770
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 771..796
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 797..817
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 818..861
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 862..882
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 883..967
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..210
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12812529,
FT ECO:0000303|PubMed:12906855, ECO:0000303|PubMed:16141072"
FT /id="VSP_026047"
FT VAR_SEQ 211..309
FT /note="GAENQPNSPRFYGKPDYPGAEEGDGYSSSKSLAVIRRGNGPFGMLGRRNEDY
FT PEGIGMVSMEMAGDPRNGYVNPAYTRRISPVCPDRNLLLCAHDWHTS -> MQSDDQVD
FT EIIIEVENVPSGVQNHLVSSQIALRKSSANPAFCVLSSSAADRVDCQIFNPGNDRRHNR
FT LLRFSSLNESISQIYHGSECLVTDESCTFHET (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12812529,
FT ECO:0000303|PubMed:12906855, ECO:0000303|PubMed:16141072"
FT /id="VSP_026048"
FT CONFLICT 527
FT /note="I -> V (in Ref. 1; AAP69873)"
FT /evidence="ECO:0000305"
FT CONFLICT 658
FT /note="V -> I (in Ref. 1; AAP69873 and 4; AAH46390)"
FT /evidence="ECO:0000305"
FT CONFLICT 793
FT /note="M -> V (in Ref. 1; AAP69873)"
FT /evidence="ECO:0000305"
FT CONFLICT 926
FT /note="N -> D (in Ref. 1; AAP69873)"
FT /evidence="ECO:0000305"
FT CONFLICT 947
FT /note="E -> D (in Ref. 4; AAH46390)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 967 AA; 111070 MW; 92B5BE50F69F3CC4 CRC64;
MSSFHQNSSY MEDADYPGYS GSRNHTHNYL ETQDYPEFPG SQNNPGFHHP RSNPHSSGSR
TNPDYPYFQE EPDYPGSLSN TVYQDTRSHP FSAHSRTRPD YESSIEPDYN DFRSESYHAG
LPMEPDYPGS QSHPGFAGVG RSSMNYTGPR TNLGYLGSLE EPDYPGAQDN SYYHSGSRPH
SNLPGSRRDA GYAGSRINSY PDDLGEPDYP GAENQPNSPR FYGKPDYPGA EEGDGYSSSK
SLAVIRRGNG PFGMLGRRNE DYPEGIGMVS MEMAGDPRNG YVNPAYTRRI SPVCPDRNLL
LCAHDWHTSV QGQKLIASLI PMTTRDRIKT IRNQPRTMQE KRELRKIVDK EKNKQSHGIL
EANCCAQCLG SLSLTYRRTR NGLSELLNYI TLWQKRFKVI GGKFGTSVLS YFSFLRWLLK
FNIFSFVMNF SFIIIPQFTV GAKNTLQFTG LEFFTGAGYF GDTVMYYGFY TNSTIRHRMG
GASYNMQLAY IFTIGACLVV CFFSLLFSMA KYFRNNFINP HIYSRGIAKL IFCWDFTVTH
EKAVKLKQKN LSTEIRENLS ELRQENYRLT FNQQLTRFSA HVAAWLVSTG VTAACCVAVY
YLAEYNSEFL KTHRNPGAVL LLPFVVSCIN LAVPRFYSMF RLVERYEIPR QEVYVLLVRN
IFLKISIVGI LCYYWLNIVA LSGEECWETL IGQDIYRLLL MDFVFSLADS LLGEFLRRLI
GMKFTSLSLQ EFDIARNVLE LIYAQTLTWL GIFFCPLLPF IQMITLFIMF YVKNVSLMMN
FQPPSKAWRA SQMITFFIFL LFFPSFTGVL CTLAITIWRL KPSADCGPFR GLPSFIQSIY
SWIDTLSRRP GYLWVVWIYQ NLIGSVHFFF ILTLIVLIIT YLYWQITEGR KVMIRLLHEQ
IINEGKDKMF LIEKLTKLQD MEKRVNPSAL DLERREVEPQ IPLHLEELGA APDLRLRRSA
QEENPIA
