TMC5_RAT
ID TMC5_RAT Reviewed; 965 AA.
AC Q5M7W4;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Transmembrane channel-like protein 5;
GN Name=Tmc5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-515.
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Probable ion channel. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5M7W4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5M7W4-2; Sequence=VSP_026049, VSP_026050;
CC -!- SIMILARITY: Belongs to the TMC family. {ECO:0000305}.
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DR EMBL; BC088406; AAH88406.1; -; mRNA.
DR EMBL; AABR03003518; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03003346; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001012216.1; NM_001012216.1. [Q5M7W4-2]
DR AlphaFoldDB; Q5M7W4; -.
DR BioGRID; 265315; 1.
DR IntAct; Q5M7W4; 1.
DR MINT; Q5M7W4; -.
DR STRING; 10116.ENSRNOP00000059631; -.
DR iPTMnet; Q5M7W4; -.
DR PhosphoSitePlus; Q5M7W4; -.
DR PaxDb; Q5M7W4; -.
DR PRIDE; Q5M7W4; -.
DR Ensembl; ENSRNOT00000055103; ENSRNOP00000051978; ENSRNOG00000036760. [Q5M7W4-2]
DR Ensembl; ENSRNOT00000096952; ENSRNOP00000097174; ENSRNOG00000036760. [Q5M7W4-1]
DR GeneID; 365360; -.
DR KEGG; rno:365360; -.
DR UCSC; RGD:1307037; rat. [Q5M7W4-1]
DR CTD; 79838; -.
DR RGD; 1307037; Tmc5.
DR eggNOG; ENOG502QQB2; Eukaryota.
DR GeneTree; ENSGT01050000244894; -.
DR InParanoid; Q5M7W4; -.
DR OrthoDB; 1048914at2759; -.
DR PhylomeDB; Q5M7W4; -.
DR PRO; PR:Q5M7W4; -.
DR Proteomes; UP000002494; Chromosome 1.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR InterPro; IPR038900; TMC.
DR InterPro; IPR012496; TMC_dom.
DR PANTHER; PTHR23302; PTHR23302; 2.
DR Pfam; PF07810; TMC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Ion channel; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..965
FT /note="Transmembrane channel-like protein 5"
FT /id="PRO_0000289968"
FT TOPO_DOM 1..417
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 468..484
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 506..578
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 600..613
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 614..634
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 635..657
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 658..678
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 679..691
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 692..712
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 713..747
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 748..768
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 769..794
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 795..815
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 816..859
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 860..880
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 881..965
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..105
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT VAR_SEQ 1..207
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026049"
FT VAR_SEQ 208..307
FT /note="GAENQPNSPDFYGKPDYPGAEEGDVYSPSKTLGVIGRSRGSFGILGREDGDY
FT PEGIEMASLGMAGDPRNGYVNPAYMRGSSPVCPDRNLLLCARDWNTSP -> MLSDDQV
FT NEIIIEVENVPSGVQRNVSSNQITLRKSSAKPNFSMLSSSTADTIDCQIFSHGNDRRHN
FT RLLRFSSLNDSISQIYHGPECLGMDEACTFHETV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026050"
SQ SEQUENCE 965 AA; 110718 MW; F27C86122F38E7B4 CRC64;
MSSFHKNSSY MEDPDYPGYS GSQNHTQNYL RTQDDLEFPG CLDNPGFHHP RRNPYSSDSR
TNPDYHHSLA EPDYPGSPSD ADYQNTRCHP RSAHPRTRPD YEPDYEPDYN DFQSESYHPD
LSMEPDYPGS HGHPGFAGVR SSVNSTGPRT NLGYLDLEEP DYPGAQGNSY HSGPRSHSNL
PGSRRNAGYA GSRINSYPDS LGEPDYPGAE NQPNSPDFYG KPDYPGAEEG DVYSPSKTLG
VIGRSRGSFG ILGREDGDYP EGIEMASLGM AGDPRNGYVN PAYMRGSSPV CPDRNLLLCA
RDWNTSPQGQ KLIASLVPMT SRDRIKTIRN QPRTMQEKRE LRKIVDKEKN KQSHGTFEAN
CCAQCLSSLS LAYRRTKSSL SELLNYISLW QKRFKVIGGK FGTSVLSYFS FLRWLLKFNI
FSFVMNFSFI IIPQFTVGEK NTLQFTGLEF FTGAGYFRET VMYYGFYTNS TIRHRMGGAS
YNMQLAYIFT IGACLVICFF SLLFSMAKYF RNNFINPHIY SRGIAKLIFC WDFTVTHEKA
VKLKQKNLST EIRENLSEIR QENDRLTLNQ KLTRFSVHVA AWLVSTGITA ACCVAVYYLA
EYNSEFLKTH KNPGAVLLLP FVVSCINLAV PRFYSMFRLV ERYEIPRQEV YVLLIRNIFL
KISIVGILCY YWLNIVALSG EECWETLIGQ DIYRLLLMDF VFSLADSLLG EFLRRLIGMK
FITSLSLQEF DIARNVLELI YAQTLAWLGI FFCPLLPFIQ MITLFIMFYV KNVSLMMNFQ
PPSKAWRASQ MITFFIFLLF FPSFTGVLCT LAITIWRLKP SADCGPFRGL PSFIQSIYSW
IDTLSHRPGY LWVVWIYQNL IGSVHFFFIL TLIVLIITYL YWQITEGRKV MIRLLHEQII
NEGKDKMFLI EKLTKLQDIE RRANPSTLVL ERREVEQQSP LHLEELDAAP DLRLRRSMQE
ENAIA
