BTB6A_DANRE
ID BTB6A_DANRE Reviewed; 540 AA.
AC A9JRD8;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2017, sequence version 2.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=BTB/POZ domain-containing protein 6-A;
GN Name=btbd6a; ORFNames=zgc:172197;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, ALTERNATIVE SPLICING, INTERACTION WITH CUL3 AND ZBTB16,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=20080956; DOI=10.1101/gad.554510;
RA Sobieszczuk D.F., Poliakov A., Xu Q., Wilkinson D.G.;
RT "A feedback loop mediated by degradation of an inhibitor is required to
RT initiate neuronal differentiation.";
RL Genes Dev. 24:206-218(2010).
CC -!- FUNCTION: Adapter protein for the cul3 E3 ubiquitin-protein ligase
CC complex. Promotes the export of zbtb16/plzf from the nucleus to the
CC cytoplasm and targets zbtb16/plzf for ubiquitination and degradation.
CC Up-regulates neurog1 expression and antagonizes zbtb16/plzf, to promote
CC neurogenesis. {ECO:0000269|PubMed:20080956}.
CC -!- SUBUNIT: Interacts with cul3. Interacts (via BTB domain) with
CC zbtb16/plzf. {ECO:0000269|PubMed:20080956}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20080956}. Nucleus
CC {ECO:0000269|PubMed:20080956}. Note=Present mainly, but not excusively
CC in the cytoplasm. {ECO:0000269|PubMed:20080956}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=btbd6a1;
CC IsoId=A9JRD8-1; Sequence=Displayed;
CC Name=2; Synonyms=btbd6a2;
CC IsoId=A9JRD8-2; Sequence=VSP_059269;
CC -!- TISSUE SPECIFICITY: Expressed in the developing central nervous system.
CC {ECO:0000269|PubMed:20080956}.
CC -!- DEVELOPMENTAL STAGE: Expressed during primary neurogenesis. At the 3
CC somite stage (3s), expressed in rostrocaudal stripes in the posterior
CC neural plate characteristic of the lateral zone, intermediate zone, and
CC medial zone of primary neurogenesis. At 14s and 20 hpf, widely
CC expressed in the spinal cord. By 24 hpf, becomes restricted to the
CC posterior spinal cord. Expression also occurs in a dynamic segmental
CC pattern in the hindbrain, at the mid-hindbrain boundary, cranial
CC ganglia, midbrain, and forebrain. Isoform 1: Expressed throughout early
CC development. Isoform 2: up-regulated from 9 hpf, when neurogenesis is
CC initiated. {ECO:0000269|PubMed:20080956}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of both isoform 1 and
CC isoform 2 leads to a major decrease in the expression of neurog1 and
CC downstream markers of neuronal differentiation. Knockdown of either
CC isoform alone leads to a mild decrease in the expression of late
CC markers of neurogenesis, but has no detectable effect on neurog1
CC expression. {ECO:0000269|PubMed:20080956}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI55623.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BC155622; AAI55623.1; ALT_INIT; mRNA.
DR RefSeq; NP_001108397.1; NM_001114925.1.
DR AlphaFoldDB; A9JRD8; -.
DR SMR; A9JRD8; -.
DR BioGRID; 283159; 2.
DR STRING; 7955.ENSDARP00000094404; -.
DR PaxDb; A9JRD8; -.
DR GeneID; 100141360; -.
DR KEGG; dre:100141360; -.
DR CTD; 100141360; -.
DR ZFIN; ZDB-GENE-080218-26; btbd6a.
DR eggNOG; KOG2075; Eukaryota.
DR InParanoid; A9JRD8; -.
DR OrthoDB; 373064at2759; -.
DR PhylomeDB; A9JRD8; -.
DR PRO; PR:A9JRD8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0022008; P:neurogenesis; IBA:GO_Central.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:ZFIN.
DR Gene3D; 2.60.120.820; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR012983; PHR.
DR InterPro; IPR038648; PHR_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF08005; PHR; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Neurogenesis; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..540
FT /note="BTB/POZ domain-containing protein 6-A"
FT /id="PRO_0000380249"
FT DOMAIN 138..208
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT VAR_SEQ 1..56
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:20080956"
FT /id="VSP_059269"
SQ SEQUENCE 540 AA; 59805 MW; 7C89C74672D813DA CRC64;
MPAAPECRLS NHGRIMKCVT FLLLLPETLK KLKRASKHPG RLSVCYNILT LSLKKRMAAE
LYPVSDHATL QKSGAVMLSL PEKKRNVEPV SQTTASIATT PTTEQNINNN NVEIPSWHSA
HPTLRERNAL MFNNEQMADV HFIVGPPGES QRVPAHKYVL AVGSSVFCAM FYGDLAEGDS
DIHIPDVEPA AFLILLKYMY SDEIELAPDT VLATLYAAKK YLVSALARAC VGFLETSLEA
RNACVLLSQS RLFEEPELTQ RCWEVIDAQA ELALRSEGFS EIDLPTLESI LHRETLNVKE
SVVFQAVLGW ADAECRRQGL SPTSQNQRSV LGKALHLVRL PSMTLQEFAD GAAQVDILTL
EETHSIFLWY TAATKPSLGF PVNAREGLTA QRCHRFQSSA YRSNQWRYRG RCDSIQFAVD
KRVFIAGLGL YGSSGGKAEY SVRIELKRQG VLLAQNLTKF VSDGSSSTFP VWFEHPVQVE
QDAFYTVSAV LDGSELSYFG QEGMTEVQCG KVTFQFQCSS DSTNGTGVQG GQIPELIFYA
