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TMCA_METKA
ID   TMCA_METKA              Reviewed;         855 AA.
AC   Q8TYZ5;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=tRNA(Met) cytidine acetyltransferase TmcA {ECO:0000255|HAMAP-Rule:MF_01886};
DE            EC=2.3.1.193 {ECO:0000255|HAMAP-Rule:MF_01886};
GN   Name=tmcA {ECO:0000255|HAMAP-Rule:MF_01886}; OrderedLocusNames=MK0146;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at
CC       the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl
CC       donor and ATP (or GTP). {ECO:0000255|HAMAP-Rule:MF_01886}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + ATP + cytidine(34) in elongator tRNA(Met) + H2O =
CC         ADP + CoA + H(+) + N(4)-acetylcytidine(34) in elongator tRNA(Met) +
CC         phosphate; Xref=Rhea:RHEA:43788, Rhea:RHEA-COMP:10693, Rhea:RHEA-
CC         COMP:10694, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216;
CC         EC=2.3.1.193; Evidence={ECO:0000255|HAMAP-Rule:MF_01886};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01886}.
CC   -!- SIMILARITY: Belongs to the RNA cytidine acetyltransferase family. TmcA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01886}.
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DR   EMBL; AE009439; AAM01363.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TYZ5; -.
DR   SMR; Q8TYZ5; -.
DR   STRING; 190192.MK0146; -.
DR   PRIDE; Q8TYZ5; -.
DR   EnsemblBacteria; AAM01363; AAM01363; MK0146.
DR   KEGG; mka:MK0146; -.
DR   PATRIC; fig|190192.8.peg.146; -.
DR   HOGENOM; CLU_004652_1_0_2; -.
DR   OMA; NEPDDLG; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051392; F:tRNA N-acetyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051391; P:tRNA acetylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0002101; P:tRNA wobble cytosine modification; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01886; tRNA_acetyltr_TmcA; 1.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   InterPro; IPR007807; Helicase_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR032672; TmcA/NAT10/Kre33.
DR   InterPro; IPR013562; TmcA_N.
DR   InterPro; IPR024914; tRNA_acetyltr_TmcA.
DR   PANTHER; PTHR10925; PTHR10925; 1.
DR   Pfam; PF08351; DUF1726; 1.
DR   Pfam; PF13718; GNAT_acetyltr_2; 2.
DR   Pfam; PF05127; Helicase_RecD; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; ATP-binding; Cytoplasm; Nucleotide-binding;
KW   Reference proteome; RNA-binding; Transferase; tRNA processing;
KW   tRNA-binding.
FT   CHAIN           1..855
FT                   /note="tRNA(Met) cytidine acetyltransferase TmcA"
FT                   /id="PRO_0000403131"
FT   DOMAIN          480..663
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         260
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         286..295
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         438
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         590..592
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         597..603
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         630
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
FT   BINDING         637
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01886"
SQ   SEQUENCE   855 AA;  98136 MW;  D3A95DFEB6903278 CRC64;
     MPDKAEVFFD EGVLDFADVD EIVLDVGEEA LAEALAHRHR RMIVFQGDEG KAEAAGVVTA
     GAADVLFDVR DRPISVLYVT DSLKEDTYAR ERYEEFRRVL EGFAEEANFE YELEALTFSG
     SKRALGTTWD LMVIDLSYDL DPDAIGRLVE TVRGGGLVIF QTPPFDRWRN MWTAFHKSLV
     TPPYTLDHVG KRFNRRFIRK LKEHDGVWIV DTDEWTAEPE PSEDVDLEVE VKRRERPDLD
     PPDDAVLPEE LYRMCATEDQ FRALIRFEEL LESNGKTALI LTADRGRGKS ALLGIAVAGA
     GVTTDVYDVV VTASEPENVA VLFEFLLEAL RELGVEYDVE RDDKGNIVYV ETDDFVVEYE
     RPSEASEIEC DLMVVDEAAS IHVPILERIL DNNDKVVYSS TIHGYEGAGR GFSVRFLQNV
     RKRRDVRLIE FKMHEPIRYD SDDPIERWLF DTLLLDAEPA DLDKEDLECV KEMRVEFEKP
     DLRYWFEDPE GEEELRQFIG IYVMAHYRNR PSDVMVLADA PHHEAYALKT ETGKIVTALQ
     VAREGTIPRD VITKMRRGYR PPGNVIPDLM VQHHDALDFP RMKGLRIVRI ATHPDIMRHG
     LGSRALKELA KIAKKKDYDW IGTGFGANEE LTRFWLRNGF VPVHISPNRN PVSGEYSVAV
     IRPISEEAEE IINRANFEFR IKLADWLGET HRDLEPEVAR LLFEPMSSLR YRPTLTEGQL
     RRLKKYADMV HTYEIAADAV RELAKAYFLD TEDRPELSEE EELLLITKCL QRWKWADVAD
     VLGEEVPDLM RSLRDLVGLL YEEYKEDLQR SAAVEGIRKA VERLADKGLT GTVIVEVEEG
     EPKEVIIRRE ERLEL
 
 
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