TMCB_DICDI
ID TMCB_DICDI Reviewed; 1416 AA.
AC Q5FBC3; Q54HV2;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Tiny macrocysts protein B;
DE AltName: Full=Gamete enriched gene 04 protein;
DE AltName: Full=cAMP pathway regulator tmcB;
GN Name=tmcB; Synonyms=GEG04; ORFNames=DDB_G0289207;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=AX3-1;
RX PubMed=15817229; DOI=10.1016/j.mod.2004.11.015;
RA Muramoto T., Takeda S., Furuya Y., Urushihara H.;
RT "Reverse genetic analyses of gamete-enriched genes revealed a novel
RT regulator of the cAMP signaling pathway in Dictyostelium discoideum.";
RL Mech. Dev. 122:733-743(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION.
RX PubMed=12963116; DOI=10.1016/s0925-4773(03)00136-9;
RA Muramoto T., Suzuki K., Shimizu H., Kohara Y., Kohriki E., Obara S.,
RA Tanaka Y., Urushihara H.;
RT "Construction of a gamete-enriched gene pool and RNAi-mediated functional
RT analysis in Dictyostelium discoideum.";
RL Mech. Dev. 120:965-975(2003).
CC -!- FUNCTION: Regulator of the cAMP signaling pathway specific to sexual
CC development. Controls the levels of external cAMP by regulating the
CC expression of phosphodiesterase pdsA and its inhibitor pdiA.
CC {ECO:0000269|PubMed:15817229}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expression in fusion-competent cells is about 6
CC times higher than fusion-incompetent cells. Remains high until 8 hours
CC of sexual development, and then slightly decreases. In the asexual
CC development, the transcript level increases temporarily during the pre-
CC aggregation stage (4 hours), and then returns to the basal level.
CC {ECO:0000269|PubMed:15817229}.
CC -!- DISRUPTION PHENOTYPE: Null cells produce tiny macrocysts and have
CC increased numbers of peripheral cells during sexual development.
CC Macrocyst formation by null cells is much less sensitive to the
CC addition of exogenous cAMP compared to wild-type. The mRNA level of
CC phosphodiesterase (pdsA) is higher and that of its inhibitor (pdiA) is
CC lower in the null cells compared to wild-type during sexual
CC development. The expression of regA, which regulates the intercellular
CC cAMP signaling pathway is not affected. Null cells show less extensive
CC cell aggregation toward the zygote giant cells and many cells remain
CC around poorly formed macrocysts. The expression of the carA is
CC unchanged or slightly higher in the null cells, while that of the carC
CC is much reduced. Chemotaxis toward cAMP during asexual development is
CC indistinguishable between the wild-type and null cells.
CC {ECO:0000269|PubMed:15817229}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB187035; BAD89539.1; -; mRNA.
DR EMBL; AAFI02000131; EAL62837.1; -; Genomic_DNA.
DR RefSeq; XP_636337.1; XM_631245.1.
DR AlphaFoldDB; Q5FBC3; -.
DR PaxDb; Q5FBC3; -.
DR EnsemblProtists; EAL62837; EAL62837; DDB_G0289207.
DR GeneID; 8627010; -.
DR KEGG; ddi:DDB_G0289207; -.
DR dictyBase; DDB_G0289207; tmcB.
DR eggNOG; ENOG502S3MF; Eukaryota.
DR HOGENOM; CLU_253378_0_0_1; -.
DR InParanoid; Q5FBC3; -.
DR OMA; YMEVSDK; -.
DR PhylomeDB; Q5FBC3; -.
DR PRO; PR:Q5FBC3; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019933; P:cAMP-mediated signaling; IMP:dictyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:dictyBase.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR SMART; SM00386; HAT; 2.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1416
FT /note="Tiny macrocysts protein B"
FT /id="PRO_0000383598"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 706..726
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 953..973
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1179..1199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1325..1345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1358..1378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 356..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..691
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1018..1103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1119..1144
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..371
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1019..1038
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1041..1103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1416 AA; 159969 MW; 4DDA5105CB56DD03 CRC64;
MSSTKSGSSK YSSGSSISLI MGFSRSLEKG SFGVLYAMIK GNTFPRILTI LSMLIEFCQL
SSFGFKHQYP WGGDAGYYLK RIMSPVSHPS DVFGYLGFTI LFWIAVGLLI LGFLNIWYVA
YQFYRGKIAN IWIIRTLRWF VSFSVAVLFI PIISLLLIGL DCEGSSEGTI LRKFDNETIQ
CFRGANLPIA VISIILIVVF SIVAFTSSAT YYEYDTNVKS RFSKPHARFD VTVLFAKFVF
AFFNSLVDFV PWLTSITFFV FMVILTFGSI IVLPYNNQRL NQVRSGFYTV VLWVSFMTLV
TMGINDETSP ATCYITIVGV FFAFPIGFFS NMFYFKWLSS KVSDIQILNQ SPSMDLKDAN
KKGKRNSVEK ESSPTSKVTW GKQPITLGSK RQIIFPFFKK KFVMSFFVEI MARSILNNNN
EGVSSSSNES IERANNLYQC GLQYFPNSDL LWMAYCNFLF TVRKDRHIGY AALEKLRRMK
PSFDVRFFIY QRDKEREQIM DSDLRGPEHT GKIQDFVSYM EFKKLYYGAK RHHVKCLNYI
KRFWGHLLHE TVDLHRLSDL SGRIATTENK ANESYERLLA LNPNSVRVLR DYSQFLEEVV
KDKESSYKLQ KKAEAIEDIM SKSQTTDFKI SDIKAIDSDE DDIEKTLQEP TAIQLAKIDA
DIEKSGSKSG SSKSKDDSSE SSSSSKGRRG KYKEFQQSNA INKLSWLMIG TTCCCIIFLI
VVLVVFRGLE VKHTNAYQGI ISITDCANEA VQMGMHLNAM QAYALVAGVG NNTVEASLIE
IARRRALVKR GIIIMKNIHD AIYWGEGNPV SYVGDNLSQL KARDGYDIFD IGSVIFTFDQ
FNRSSTLLDN KQMIELYSQP SVNMSVLVAP PGSNTTSLYT QTYNAWKAGN AFIESAMLAS
DVTFDELRYS VNQNPNFKFV TINAPVIMPD IYNKVQTAYI DSLVSDITGT LDAILYTWCG
IFAVLFLICA VLFRPIVRKI SREKIRTLVL FSLAPKDVVV KLSTKKIKMV SLDSGSERDN
LFDTTDDDGR DDHLGEDDNN IQTPVGDNNN NNNNNNNNNN NNGSISSNVT KSDGEIGSDG
VRSSSGSNVL NTSSPYRDRR PLMDSTSVLA STATLNNRNV RLQAKDEEIT NGGGERKGSD
ATRTALSEDK KYGWDGKSKR NLNKKSLKSI LGRLHWSYIL ATFLLFGFIT MGIWVTFTVV
FNNSQSGYTL GKSCSRSLNS RVINYYVAEL YTYSQYVSTQ NMVDAITELQ SSHQSLPYLE
EVRPLMEGSY GCWALNKSTC LPPSSMYYEE VNSGLDRVVD SLAKKAINLA YTDQAALLDS
PDLQWFLALI DYIFIGLDTA TFTYFDYFLE KQEWANTVLT AILSVSCVIL LVVHVVLFRP
FMNHLRIQHI HTLALLRLAP DDIRYMEVSD KVIDED
