TMCC3_MOUSE
ID TMCC3_MOUSE Reviewed; 477 AA.
AC Q8R310; Q501N6;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transmembrane and coiled-coil domain protein 3 {ECO:0000250|UniProtKB:Q9ULS5};
GN Name=Tmcc3 {ECO:0000312|MGI:MGI:2442900};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=129, Czech II, and NMRI; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-253, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=27697108; DOI=10.5483/bmbrep.2016.49.11.151;
RA Sohn W.J., Kim J.Y., Kim D., Park J.A., Lee Y., Kwon H.J.;
RT "Expression and characterization of transmembrane and coiled-coil domain
RT family 3.";
RL BMB Rep. 49:629-634(2016).
CC -!- SUBUNIT: May form homodimers and heterodimers with TMCC2 or TMCC3 via
CC the coiled-coil domains. Interacts with ribosomal proteins RPL4 and
CC RPS6. {ECO:0000250|UniProtKB:Q9ULS5}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9ULS5}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Concentrates in discrete patches along peripheral
CC endoplasmic reticulum tubules. {ECO:0000250|UniProtKB:Q9ULS5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8R310-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R310-2; Sequence=VSP_025999;
CC -!- DEVELOPMENTAL STAGE: At 12.5 dpc, weak expression in the developing
CC lung and hindbrain, high expression in the tongue mesenchyme
CC (PubMed:27697108). At 14.5 dpc, expressed in the trigeminal ganglion,
CC oral epithelium and hindbrain (PubMed:27697108). Also detected in lung,
CC kidney and somites (PubMed:27697108). {ECO:0000269|PubMed:27697108}.
CC -!- SIMILARITY: Belongs to the TEX28 family. {ECO:0000305}.
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DR EMBL; AK132545; BAE21229.1; -; mRNA.
DR EMBL; BC026867; AAH26867.1; -; mRNA.
DR EMBL; BC057202; AAH57202.1; -; mRNA.
DR EMBL; BC095951; AAH95951.1; -; mRNA.
DR CCDS; CCDS24134.1; -. [Q8R310-1]
DR CCDS; CCDS48675.1; -. [Q8R310-2]
DR RefSeq; NP_001162155.1; NM_001168684.1. [Q8R310-2]
DR RefSeq; NP_742048.2; NM_172051.3. [Q8R310-1]
DR RefSeq; NP_796000.1; NM_177026.1. [Q8R310-2]
DR RefSeq; XP_006513815.1; XM_006513752.1.
DR RefSeq; XP_006513816.1; XM_006513753.1.
DR RefSeq; XP_006513817.1; XM_006513754.3. [Q8R310-2]
DR AlphaFoldDB; Q8R310; -.
DR SMR; Q8R310; -.
DR BioGRID; 235592; 1.
DR STRING; 10090.ENSMUSP00000063264; -.
DR iPTMnet; Q8R310; -.
DR PhosphoSitePlus; Q8R310; -.
DR jPOST; Q8R310; -.
DR MaxQB; Q8R310; -.
DR PaxDb; Q8R310; -.
DR PeptideAtlas; Q8R310; -.
DR PRIDE; Q8R310; -.
DR ProteomicsDB; 259125; -. [Q8R310-1]
DR ProteomicsDB; 259126; -. [Q8R310-2]
DR Antibodypedia; 2821; 78 antibodies from 19 providers.
DR DNASU; 319880; -.
DR Ensembl; ENSMUST00000065060; ENSMUSP00000063264; ENSMUSG00000020023. [Q8R310-1]
DR Ensembl; ENSMUST00000117460; ENSMUSP00000112669; ENSMUSG00000020023. [Q8R310-2]
DR Ensembl; ENSMUST00000117929; ENSMUSP00000112401; ENSMUSG00000020023. [Q8R310-2]
DR Ensembl; ENSMUST00000121471; ENSMUSP00000113122; ENSMUSG00000020023. [Q8R310-2]
DR GeneID; 319880; -.
DR KEGG; mmu:319880; -.
DR UCSC; uc007gvt.2; mouse. [Q8R310-1]
DR CTD; 57458; -.
DR MGI; MGI:2442900; Tmcc3.
DR VEuPathDB; HostDB:ENSMUSG00000020023; -.
DR eggNOG; KOG3850; Eukaryota.
DR GeneTree; ENSGT00940000157275; -.
DR HOGENOM; CLU_019951_0_0_1; -.
DR InParanoid; Q8R310; -.
DR OMA; TECSKSG; -.
DR OrthoDB; 1175041at2759; -.
DR PhylomeDB; Q8R310; -.
DR TreeFam; TF316292; -.
DR BioGRID-ORCS; 319880; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Tmcc3; mouse.
DR PRO; PR:Q8R310; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8R310; protein.
DR Bgee; ENSMUSG00000020023; Expressed in placenta labyrinth and 244 other tissues.
DR ExpressionAtlas; Q8R310; baseline and differential.
DR Genevisible; Q8R310; MM.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071889; F:14-3-3 protein binding; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR InterPro; IPR019394; TEX28/TMCC.
DR PANTHER; PTHR17613; PTHR17613; 1.
DR Pfam; PF10267; Tmemb_cc2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..477
FT /note="Transmembrane and coiled-coil domain protein 3"
FT /id="PRO_0000184600"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 63..83
FT /evidence="ECO:0000255"
FT COILED 112..149
FT /evidence="ECO:0000255"
FT COILED 284..398
FT /evidence="ECO:0000255"
FT COMPBIAS 254..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 253
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..31
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025999"
SQ SEQUENCE 477 AA; 53722 MW; 8848410F6E74A217 CRC64;
MPGSDTALTV DRTYSDPGRH HRCKSRVDRH DMNTLSLPLN IRRGGSDTNL NFDVPDGILD
FHKVKLNADS LRQKILKVTE QIKIEQTSRD GNVAEYLKLV SSADKQQAGR IKQVFEKKNQ
KSAHSIAQLQ KKLEQYHRKL REIEQNGVTR SSKDISKDSL KEIHHSLKDA HVKSRTAPHC
LESSKSSMPG VSLTPPVFVF NKSREFANLI RNKFGSADNI AHLKNSLEEF RPEASPRAYG
GSATIVNKPK YGSDDECSSG TSGSADSNGN QSFGAGGTST LDSQGKIAKI MEELREIKVT
QTQLAEDIEA LKVQFKREYG FISQTLQEER YRYERLEDQL HDLTELHQHE TANLKQELAS
AEEKVAYQAY ERSRDIQEAL ESCQTRISKL ELHQQEQQTL QTDAVNAKVL LGKCINVVLA
FMTVILVCVS TLAKFVSPMM KSRSHILGTF FAVTLLAIFC KNWDHILCAI ERIIIPR
