TMCC_DICDI
ID TMCC_DICDI Reviewed; 1400 AA.
AC Q5FBC4; Q54HS5;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Tiny macrocysts protein C;
DE AltName: Full=Gamete enriched gene 10 protein;
DE AltName: Full=HAT repeat-containing protein tmcC;
GN Name=tmcC; Synonyms=GEG10; ORFNames=DDB_G0289253;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX3-1;
RX PubMed=15817229; DOI=10.1016/j.mod.2004.11.015;
RA Muramoto T., Takeda S., Furuya Y., Urushihara H.;
RT "Reverse genetic analyses of gamete-enriched genes revealed a novel
RT regulator of the cAMP signaling pathway in Dictyostelium discoideum.";
RL Mech. Dev. 122:733-743(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION.
RX PubMed=12963116; DOI=10.1016/s0925-4773(03)00136-9;
RA Muramoto T., Suzuki K., Shimizu H., Kohara Y., Kohriki E., Obara S.,
RA Tanaka Y., Urushihara H.;
RT "Construction of a gamete-enriched gene pool and RNAi-mediated functional
RT analysis in Dictyostelium discoideum.";
RL Mech. Dev. 120:965-975(2003).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expression in fusion-competent cells is about 6
CC times higher than fusion-incompetent cells. Remains high until 8 hours
CC of sexual development, and then slightly decreases. In the asexual
CC development, the transcript level increases temporarily during the pre-
CC aggregation stage (4 hours), and then returns to the basal level.
CC {ECO:0000269|PubMed:15817229}.
CC -!- DISRUPTION PHENOTYPE: Null cells have increased numbers of peripheral
CC cells during sexual development. Indistinguishable from the wild-type
CC cells with respect to the cAMP response. {ECO:0000269|PubMed:15817229}.
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DR EMBL; AB187034; BAD89538.1; -; mRNA.
DR EMBL; AAFI02000132; EAL62814.1; -; Genomic_DNA.
DR RefSeq; XP_636256.1; XM_631164.1.
DR AlphaFoldDB; Q5FBC4; -.
DR PaxDb; Q5FBC4; -.
DR EnsemblProtists; EAL62814; EAL62814; DDB_G0289253.
DR GeneID; 8627037; -.
DR KEGG; ddi:DDB_G0289253; -.
DR dictyBase; DDB_G0289253; tmcC.
DR eggNOG; ENOG502S3MF; Eukaryota.
DR HOGENOM; CLU_253378_0_0_1; -.
DR InParanoid; Q5FBC4; -.
DR OMA; SHIVEIM; -.
DR PhylomeDB; Q5FBC4; -.
DR PRO; PR:Q5FBC4; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0140253; P:cell-cell fusion; IMP:dictyBase.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR SMART; SM00386; HAT; 2.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1400
FT /note="Tiny macrocysts protein C"
FT /id="PRO_0000383599"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 726..746
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 975..995
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1162..1182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1342..1362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 367..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 683..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 370..389
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1400 AA; 159154 MW; F6D258543AB807C5 CRC64;
MSSHKSTTKS SSSGSSMSLI LGFSKSMEKG NYNFSKENKK NRSFGVLYAM IKGNTFPRIL
TIISLLIEFC QLSSFGFKQQ YPWGGDAGYY LKRIMSPVSH PSNLVGYNGF TIFFWIVIGL
LLLGFFNIWY VAYQFYRGKI ANIWIIRTLR WFVSTTVAVL FIPIISLLLI GLDCDYSQGG
ILKTFSNDNI YCFKGANLSI AIVSIILIIV FSIVGFTSSA TYYEYDTNVK SRFSKPHARF
DVYILFVKLI LALLNSLVDF SPWTTSIVYF ILSITLVFGS IIILPYNNQR LNQVKSGFYT
TVFWVSFMTL VTMGINDETT ATTCYITIVG AFFAFPIGYF SNRFYYNWLS SKIDQLKLPT
SSSTNDFNEI TKNEKSKTGD SKEKESSSPS KVTWGKQPIT LGSKRQIIFP FFQNKFVMSF
FVEIMVRKLL RSSNGSNGDD GISSNSNESI EHANNLYQCG LQYFPNSDLL WMAYCNFLFT
VRKDRHIGYA ALEKLRRMKP SFDVRFFIYQ RDKEREQIMD SDLRGPEHTG KIQDFVSYME
FKKLYYGAKR HHVKCLTYIK KFWGLLLHET VDLHRLSDLS GRIATTENKA NESYERLLAL
NPNSVRVLRD YSQFLEEVVK DTESSYKLQK KAEAIEDIMS KSQTTDFKTI DIKNLDNSDT
ELDQVLKQES NAIQLAKIDA DIERSGSKSG SSKSKDDSSE SSSSSKGRRG KYKEFQQSNA
INKLSWLMIG TTACCIIFLI VMLIVLRDLS VKHTHSYQGI ISITDCASEA VSIAINLNTM
QAISISAGSV AFPFEAAEAM ITQYRKQNDR SLIIMKNIHD AIYWGEGDPT SYVGDNLSKL
KSINGFDVFD IGSTIFSFEE FNRSSTLVDN KEMIDIYSTP SVNMTILVSP PGTNSTNYIT
VTQTYNAWKA GNSFYESALI ANKMSVQDIK DRAVFDPDFK FIILNAPSNI PEMYMKIQQV
YIKSLIDDIN TTLDTMLYVW VAIFCFLIIL GAVLFRPIVT KISREKIRTL VLFSLAPKDV
VFKLSSKKIK MTSLDSGSER DNLFDTTDDD AADGIDNHEH LQVDERKKND DIIDDGSIVN
RSINFGSHRR PLMNSTNVLA STTTINRGTI SNRLNEDHDK VPLIENSISN NNKGYGWDGK
SKRNLNKKSL RSVLRRLHWS YVLAIFLLFG FITMGLWVTY SVVHDNTQSG YVLGKSCSRS
LDSRIINYYI AELYTFDNDA NENEALEAVL QLQSNHQSLP YLEDVRPLME GSYGCWMLNK
SNCITSDSIY YNDVSIGLDW LVDQYTKHSV SLVNTDPSLL STSPELGWMQ AIGSDVVFQG
LDTATFTYFQ YYLSQKDWAT KVLTSVLAIS CVLLLVIHLL LFRPFMNHLR IQHIHTLALL
RLAPDDIRFM EVSDKVIDED
