TMCO1_DANRE
ID TMCO1_DANRE Reviewed; 188 AA.
AC Q6DGW9; A8E529; B0S7X3; Q568F9;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Calcium load-activated calcium channel {ECO:0000250|UniProtKB:Q9UM00};
DE Short=CLAC channel {ECO:0000250|UniProtKB:Q9UM00};
DE AltName: Full=Transmembrane and coiled-coil domain-containing protein 1 {ECO:0000250|UniProtKB:Q9UM00};
GN Name=tmco1 {ECO:0000312|ZFIN:ZDB-GENE-050417-344};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo, Liver, and Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=27212239; DOI=10.1016/j.cell.2016.04.051;
RA Wang Q.C., Zheng Q., Tan H., Zhang B., Li X., Yang Y., Yu J., Liu Y.,
RA Chai H., Wang X., Sun Z., Wang J.Q., Zhu S., Wang F., Yang M., Guo C.,
RA Wang H., Zheng Q., Li Y., Chen Q., Zhou A., Tang T.S.;
RT "TMCO1 is an ER Ca(2+) load-activated Ca(2+) channel.";
RL Cell 165:1454-1466(2016).
CC -!- FUNCTION: Calcium-selective channel required to prevent calcium stores
CC from overfilling, thereby playing a key role in calcium homeostasis
CC (PubMed:27212239). In response to endoplasmic reticulum (ER)
CC overloading, assembles into a homotetramer, forming a functional
CC calcium-selective channel, regulating the calcium content in
CC endoplasmic reticulum store (By similarity). Necessary for the
CC biogenesis and transport of multi-pass membrane proteins into the ER
CC membrane (By similarity). {ECO:0000250|UniProtKB:Q9UM00,
CC ECO:0000305|PubMed:27212239}.
CC -!- SUBUNIT: Homodimer and homotetramer. Component of the ribosome-
CC associated ER translocon complex. {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Note=The first transmembrane region is
CC required for localization to the endoplasmic reticulum.
CC {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6DGW9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6DGW9-2; Sequence=VSP_058496;
CC -!- SIMILARITY: Belongs to the TMCO1 family. {ECO:0000305}.
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DR EMBL; BX927144; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC076218; AAH76218.1; -; mRNA.
DR EMBL; BC092873; AAH92873.1; -; mRNA.
DR EMBL; BC152265; AAI52266.1; -; mRNA.
DR EMBL; BC153441; AAI53442.1; -; mRNA.
DR EMBL; BC164974; AAI64974.1; -; mRNA.
DR RefSeq; NP_001002575.1; NM_001002575.1. [Q6DGW9-1]
DR AlphaFoldDB; Q6DGW9; -.
DR SMR; Q6DGW9; -.
DR STRING; 7955.ENSDARP00000091049; -.
DR PaxDb; Q6DGW9; -.
DR Ensembl; ENSDART00000100276; ENSDARP00000091049; ENSDARG00000069099. [Q6DGW9-1]
DR Ensembl; ENSDART00000127371; ENSDARP00000106023; ENSDARG00000069099. [Q6DGW9-2]
DR Ensembl; ENSDART00000187923; ENSDARP00000147474; ENSDARG00000113013. [Q6DGW9-2]
DR GeneID; 378980; -.
DR KEGG; dre:378980; -.
DR CTD; 54499; -.
DR ZFIN; ZDB-GENE-050417-344; tmco1.
DR eggNOG; KOG3312; Eukaryota.
DR GeneTree; ENSGT00390000002659; -.
DR HOGENOM; CLU_081121_0_0_1; -.
DR InParanoid; Q6DGW9; -.
DR OMA; WADTLLI; -.
DR OrthoDB; 1506634at2759; -.
DR PhylomeDB; Q6DGW9; -.
DR TreeFam; TF315045; -.
DR PRO; PR:Q6DGW9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 8.
DR Bgee; ENSDARG00000069099; Expressed in early embryo and 30 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0032469; P:endoplasmic reticulum calcium ion homeostasis; IDA:UniProtKB.
DR GO; GO:0006983; P:ER overload response; ISS:UniProtKB.
DR InterPro; IPR002809; EMC3/TMCO1.
DR InterPro; IPR008559; TMCO1.
DR PANTHER; PTHR20917; PTHR20917; 1.
DR Pfam; PF01956; EMC3_TMCO1; 1.
DR PIRSF; PIRSF023322; DUF841_euk; 1.
DR SMART; SM01415; DUF106; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Calcium channel; Calcium transport;
KW Coiled coil; Endoplasmic reticulum; Golgi apparatus; Ion channel;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..188
FT /note="Calcium load-activated calcium channel"
FT /id="PRO_0000437215"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..90
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT INTRAMEM 134..154
FT /note="Pore-forming"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TOPO_DOM 155..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT COILED 32..89
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..23
FT /note="MSTMFADTILIVFISICTALLAE -> MQDKYSSKAALQFVSLCFT (in
FT isoform 2)"
FT /id="VSP_058496"
FT CONFLICT 34
FT /note="D -> G (in Ref. 2; AAI53442)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="R -> G (in Ref. 2; AAI53442)"
FT /evidence="ECO:0000305"
FT CONFLICT Q6DGW9-2:9
FT /note="A -> S (in Ref. 2; AAH92873/AAI64974)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 188 AA; 21310 MW; 055C7526D96BA6C7 CRC64;
MSTMFADTIL IVFISICTAL LAEGITWVLV YRTDKYKRLK AEVEKQSKKL EKKKETITES
AGRQQKKKIE RQEEKLKNNN RDLSMVRMKS MFAIGFCFTA LMGMFNSIFD GRVVAKLPFV
PLSYIQGLSH RNLLGEDYTD CSFIFLYILC TMSIRQNIQK MLGLAPSRAA TKQAGGFLGP
PPQAAKFS
