TMCO1_PIG
ID TMCO1_PIG Reviewed; 188 AA.
AC C5HGF3;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Calcium load-activated calcium channel {ECO:0000250|UniProtKB:Q9UM00};
DE Short=CLAC channel {ECO:0000250|UniProtKB:Q9UM00};
DE AltName: Full=Transmembrane and coiled-coil domain-containing protein 1 {ECO:0000250|UniProtKB:Q9UM00};
GN Name=TMCO1 {ECO:0000250|UniProtKB:Q9UM00};
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=19449125; DOI=10.1007/s11033-009-9573-8;
RA Zhang Z., Mo D., Cong P., He Z., Ling F., Li A., Niu Y., Zhao X., Zhou C.,
RA Chen Y.;
RT "Molecular cloning, expression patterns and subcellular localization of
RT porcine TMCO1 gene.";
RL Mol. Biol. Rep. 37:1611-1618(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Calcium-selective channel required to prevent calcium stores
CC from overfilling, thereby playing a key role in calcium homeostasis. In
CC response to endoplasmic reticulum (ER) overloading, assembles into a
CC homotetramer, forming a functional calcium-selective channel,
CC regulating the calcium content in endoplasmic reticulum store.
CC Component of a ribosome-associated ER translocon complex involved in
CC multi-pass membrane protein transport into the ER membrane and
CC biogenesis. Together with SEC61 and TMEM147, forms the lipid-filled
CC cavity at the center of the translocon where TMEM147 may insert
CC hydrophobic segments of mutli-pass membrane proteins from the lumen
CC into de central membrane cavity in a process gated by SEC61, and TMCO1
CC may insert hydrophobic segments of nascent chains from the cytosol into
CC the cavity. {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SUBUNIT: Homodimer and homotetramer. Homodimer under resting
CC conditions; forms homotetramers following and ER calcium overload. The
CC ribosome-associated ER translocon complex includes SEC61A1, SEC61B,
CC SEC61G, TMCO1, CCDC47, NCLN/Nicalin, NOMO and TMEM147; in the absence
CC of ribosomes, only the complex forms with NCLN/Nicalin, NOMO and
CC TMEM147 remains intact. {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Note=The first transmembrane region is
CC required for localization to the endoplasmic reticulum.
CC {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- TISSUE SPECIFICITY: Widely expressed, with high levels in liver, kidney
CC and heart, intermediate amounts in back fat, longissimus dorsi, brain,
CC lymph, large intestine, small intestine and lung, and low levels in
CC spleen and stomach. {ECO:0000269|PubMed:19449125}.
CC -!- SIMILARITY: Belongs to the TMCO1 family. {ECO:0000305}.
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DR EMBL; CU468388; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU468989; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; FJ440337; ACN82430.1; -; mRNA.
DR RefSeq; NP_001161057.1; NM_001167585.1.
DR AlphaFoldDB; C5HGF3; -.
DR SMR; C5HGF3; -.
DR STRING; 9823.ENSSSCP00000006742; -.
DR PaxDb; C5HGF3; -.
DR PeptideAtlas; C5HGF3; -.
DR PRIDE; C5HGF3; -.
DR Ensembl; ENSSSCT00000061642; ENSSSCP00000050094; ENSSSCG00000037314.
DR Ensembl; ENSSSCT00005030335; ENSSSCP00005018520; ENSSSCG00005019067.
DR Ensembl; ENSSSCT00015034596; ENSSSCP00015013695; ENSSSCG00015025949.
DR Ensembl; ENSSSCT00025042450; ENSSSCP00025018068; ENSSSCG00025031143.
DR Ensembl; ENSSSCT00030007371; ENSSSCP00030003287; ENSSSCG00030005404.
DR Ensembl; ENSSSCT00035053422; ENSSSCP00035021479; ENSSSCG00035040225.
DR Ensembl; ENSSSCT00040053500; ENSSSCP00040022288; ENSSSCG00040039848.
DR Ensembl; ENSSSCT00045042867; ENSSSCP00045029771; ENSSSCG00045025065.
DR Ensembl; ENSSSCT00050067556; ENSSSCP00050028989; ENSSSCG00050049642.
DR Ensembl; ENSSSCT00055016611; ENSSSCP00055013105; ENSSSCG00055008466.
DR Ensembl; ENSSSCT00060095236; ENSSSCP00060041210; ENSSSCG00060069735.
DR Ensembl; ENSSSCT00065073189; ENSSSCP00065031882; ENSSSCG00065053431.
DR Ensembl; ENSSSCT00070049098; ENSSSCP00070041462; ENSSSCG00070024519.
DR GeneID; 100151809; -.
DR KEGG; ssc:100151809; -.
DR CTD; 54499; -.
DR VGNC; VGNC:94048; TMCO1.
DR eggNOG; KOG3312; Eukaryota.
DR GeneTree; ENSGT00390000002659; -.
DR HOGENOM; CLU_081121_0_0_1; -.
DR InParanoid; C5HGF3; -.
DR OMA; WADTLLI; -.
DR OrthoDB; 1506634at2759; -.
DR TreeFam; TF315045; -.
DR Proteomes; UP000008227; Chromosome 4.
DR Proteomes; UP000314985; Chromosome 4.
DR Bgee; ENSSSCG00000037314; Expressed in adult mammalian kidney and 44 other tissues.
DR ExpressionAtlas; C5HGF3; baseline and differential.
DR Genevisible; C5HGF3; SS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0070588; P:calcium ion transmembrane transport; ISS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0032469; P:endoplasmic reticulum calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0006983; P:ER overload response; ISS:UniProtKB.
DR InterPro; IPR002809; EMC3/TMCO1.
DR InterPro; IPR008559; TMCO1.
DR PANTHER; PTHR20917; PTHR20917; 1.
DR Pfam; PF01956; EMC3_TMCO1; 1.
DR PIRSF; PIRSF023322; DUF841_euk; 1.
DR SMART; SM01415; DUF106; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium channel; Calcium transport; Coiled coil;
KW Endoplasmic reticulum; Golgi apparatus; Ion channel; Ion transport;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..188
FT /note="Calcium load-activated calcium channel"
FT /id="PRO_0000413773"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..90
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT INTRAMEM 138..154
FT /note="Pore-forming"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TOPO_DOM 155..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT COILED 32..89
FT /evidence="ECO:0000255"
FT MOD_RES 60
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
SQ SEQUENCE 188 AA; 21175 MW; FB77BFC4F0629EB1 CRC64;
MSTMFADTLL IVFISVCTAL LAEGITWVLV YRTDKYKRLK AEVEKQSKKL EKKKETITES
AGRQQKKKIE RQEEKLKNNN RDLSMVRMKS MFAIGFCFTA LMGMFNSIFD GRVVAKLPFT
PLSYIQGLSH RNLLGDDTTD CSFIFLYILC TMSIRQNIQK ILGLAPSRAA TKQAGGFLGP
PPPSGKFS
