TMCO1_RAT
ID TMCO1_RAT Reviewed; 188 AA.
AC Q5I0H4; Q71DI0;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Calcium load-activated calcium channel {ECO:0000250|UniProtKB:Q9UM00};
DE Short=CLAC channel {ECO:0000250|UniProtKB:Q9UM00};
DE AltName: Full=Meg-2-like protein {ECO:0000303|Ref.2};
DE AltName: Full=Transmembrane and coiled-coil domain-containing protein 1 {ECO:0000305};
GN Name=Tmco1 {ECO:0000312|RGD:1359178};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-186.
RC STRAIN=Wistar;
RA Miyata T., Inagi R., Nangaku M., Kurokawa K.;
RT "Rat partial cDNA homologous to human mesangium-predominant gene, meg-2.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Calcium-selective channel required to prevent calcium stores
CC from overfilling, thereby playing a key role in calcium homeostasis. In
CC response to endoplasmic reticulum (ER) overloading, assembles into a
CC homotetramer, forming a functional calcium-selective channel,
CC regulating the calcium content in endoplasmic reticulum store.
CC Component of a ribosome-associated ER translocon complex involved in
CC multi-pass membrane protein transport into the ER membrane and
CC biogenesis. Together with SEC61 and TMEM147, forms the lipid-filled
CC cavity at the center of the translocon where TMEM147 may insert
CC hydrophobic segments of mutli-pass membrane proteins from the lumen
CC into de central membrane cavity in a process gated by SEC61, and TMCO1
CC may insert hydrophobic segments of nascent chains from the cytosol into
CC the cavity. {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SUBUNIT: Homodimer and homotetramer. Homodimer under resting
CC conditions; forms homotetramers following and ER calcium overload. The
CC ribosome-associated ER translocon complex includes SEC61A1, SEC61B,
CC SEC61G, TMCO1, CCDC47, NCLN/Nicalin, NOMO and TMEM147; in the absence
CC of ribosomes, only the complex forms with NCLN/Nicalin, NOMO and
CC TMEM147 remains intact. {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9UM00}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9UM00}. Note=The first transmembrane region is
CC required for localization to the endoplasmic reticulum.
CC {ECO:0000250|UniProtKB:Q9UM00}.
CC -!- SIMILARITY: Belongs to the TMCO1 family. {ECO:0000305}.
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DR EMBL; BC088319; AAH88319.1; -; mRNA.
DR EMBL; AF531432; AAQ09812.1; -; mRNA.
DR RefSeq; NP_001009631.1; NM_001009631.1.
DR RefSeq; XP_017454197.1; XM_017598708.1.
DR AlphaFoldDB; Q5I0H4; -.
DR SMR; Q5I0H4; -.
DR STRING; 10116.ENSRNOP00000005277; -.
DR jPOST; Q5I0H4; -.
DR PaxDb; Q5I0H4; -.
DR PRIDE; Q5I0H4; -.
DR GeneID; 289196; -.
DR KEGG; rno:289196; -.
DR UCSC; RGD:1359178; rat.
DR CTD; 54499; -.
DR RGD; 1359178; Tmco1.
DR VEuPathDB; HostDB:ENSRNOG00000003928; -.
DR eggNOG; KOG3312; Eukaryota.
DR InParanoid; Q5I0H4; -.
DR OMA; WADTLLI; -.
DR OrthoDB; 1506634at2759; -.
DR PhylomeDB; Q5I0H4; -.
DR PRO; PR:Q5I0H4; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000003928; Expressed in liver and 20 other tissues.
DR Genevisible; Q5I0H4; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0070588; P:calcium ion transmembrane transport; ISS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0032469; P:endoplasmic reticulum calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0006983; P:ER overload response; ISS:UniProtKB.
DR InterPro; IPR002809; EMC3/TMCO1.
DR InterPro; IPR008559; TMCO1.
DR PANTHER; PTHR20917; PTHR20917; 1.
DR Pfam; PF01956; EMC3_TMCO1; 1.
DR PIRSF; PIRSF023322; DUF841_euk; 1.
DR SMART; SM01415; DUF106; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium channel; Calcium transport; Coiled coil;
KW Endoplasmic reticulum; Golgi apparatus; Ion channel; Ion transport;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..188
FT /note="Calcium load-activated calcium channel"
FT /id="PRO_0000244079"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..90
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT INTRAMEM 138..154
FT /note="Pore-forming"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT TOPO_DOM 155..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT COILED 32..89
FT /evidence="ECO:0000255"
FT MOD_RES 60
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UM00"
FT CONFLICT 45
FT /note="K -> I (in Ref. 2; AAQ09812)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 188 AA; 21175 MW; FB77BFC4F0629EB1 CRC64;
MSTMFADTLL IVFISVCTAL LAEGITWVLV YRTDKYKRLK AEVEKQSKKL EKKKETITES
AGRQQKKKIE RQEEKLKNNN RDLSMVRMKS MFAIGFCFTA LMGMFNSIFD GRVVAKLPFT
PLSYIQGLSH RNLLGDDTTD CSFIFLYILC TMSIRQNIQK ILGLAPSRAA TKQAGGFLGP
PPPSGKFS
