TMCO3_MOUSE
ID TMCO3_MOUSE Reviewed; 678 AA.
AC Q8BH01; Q3UFX1; Q7TMT5; Q8BGL4; Q8R303;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Transmembrane and coiled-coil domain-containing protein 3;
DE Flags: Precursor;
GN Name=Tmco3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Colon, Corpora quadrigemina, Hippocampus, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=129/Sv X 129SvCp, and FVB/N;
RC TISSUE=Embryonic stem cell, Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable Na(+)/H(+) antiporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BH01-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BH01-2; Sequence=VSP_020567;
CC Name=3;
CC IsoId=Q8BH01-3; Sequence=VSP_020565, VSP_020566;
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. {ECO:0000305}.
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DR EMBL; AK033499; BAC28322.1; -; mRNA.
DR EMBL; AK046065; BAC32588.1; -; mRNA.
DR EMBL; AK049974; BAC34013.1; -; mRNA.
DR EMBL; AK088866; BAC40623.1; -; mRNA.
DR EMBL; AK148252; BAE28438.1; -; mRNA.
DR EMBL; BC026890; AAH26890.1; -; mRNA.
DR EMBL; BC039955; AAH39955.1; -; mRNA.
DR EMBL; BC053487; AAH53487.1; -; mRNA.
DR CCDS; CCDS40231.1; -. [Q8BH01-1]
DR RefSeq; NP_758486.1; NM_172282.2. [Q8BH01-1]
DR RefSeq; XP_006508825.1; XM_006508762.2.
DR AlphaFoldDB; Q8BH01; -.
DR SMR; Q8BH01; -.
DR STRING; 10090.ENSMUSP00000040347; -.
DR GlyGen; Q8BH01; 3 sites.
DR iPTMnet; Q8BH01; -.
DR PhosphoSitePlus; Q8BH01; -.
DR EPD; Q8BH01; -.
DR MaxQB; Q8BH01; -.
DR PaxDb; Q8BH01; -.
DR PeptideAtlas; Q8BH01; -.
DR PRIDE; Q8BH01; -.
DR ProteomicsDB; 259421; -. [Q8BH01-1]
DR ProteomicsDB; 259422; -. [Q8BH01-2]
DR ProteomicsDB; 259423; -. [Q8BH01-3]
DR Antibodypedia; 25950; 63 antibodies from 17 providers.
DR DNASU; 234076; -.
DR Ensembl; ENSMUST00000045229; ENSMUSP00000040347; ENSMUSG00000038497. [Q8BH01-1]
DR GeneID; 234076; -.
DR KEGG; mmu:234076; -.
DR UCSC; uc009kxm.1; mouse. [Q8BH01-3]
DR UCSC; uc009kxn.1; mouse. [Q8BH01-1]
DR UCSC; uc009kxo.1; mouse. [Q8BH01-2]
DR CTD; 55002; -.
DR MGI; MGI:2444946; Tmco3.
DR VEuPathDB; HostDB:ENSMUSG00000038497; -.
DR eggNOG; KOG1650; Eukaryota.
DR GeneTree; ENSGT00390000001394; -.
DR HOGENOM; CLU_026093_0_0_1; -.
DR InParanoid; Q8BH01; -.
DR OMA; IKAHASK; -.
DR OrthoDB; 522572at2759; -.
DR PhylomeDB; Q8BH01; -.
DR TreeFam; TF329364; -.
DR BioGRID-ORCS; 234076; 6 hits in 75 CRISPR screens.
DR ChiTaRS; Tmco3; mouse.
DR PRO; PR:Q8BH01; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BH01; protein.
DR Bgee; ENSMUSG00000038497; Expressed in right kidney and 257 other tissues.
DR ExpressionAtlas; Q8BH01; baseline and differential.
DR Genevisible; Q8BH01; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR045158; KEA4/5/6-like.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR16254; PTHR16254; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Coiled coil; Glycoprotein; Ion transport;
KW Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..678
FT /note="Transmembrane and coiled-coil domain-containing
FT protein 3"
FT /id="PRO_0000249857"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 555..575
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 609..629
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 641..661
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 124..204
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 316..328
FT /note="SIVQVETLGEFGV -> EVTGAASESSSHH (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020565"
FT VAR_SEQ 329..678
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020566"
FT VAR_SEQ 409
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020567"
FT CONFLICT 79
FT /note="E -> G (in Ref. 1; BAE28438)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="D -> G (in Ref. 2; AAH53487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 678 AA; 75903 MW; 5DCFBE9A9112FF4F CRC64;
MKVLDQSLLW MLLPFFHLIA SAAEHEEVAK HAIKLHRGKG ATATQRKQWA LDSCRRLTGL
LRQKNVVLNK LKNAIRAVEK DTSLSGEEKL FQVHTFEIFQ KELNESENSI FQAIYGLQRA
LQGDYRDVVN MKESSKQRLE ALREAAIKEE TEYVELLAAE KHQVEALKNM QHQNKSLSML
DEILEDVRKA ADRLEEEIEE HAFDDNKSVK GVNFEAVLRV EEEEASSKQN MTKREVEDGL
GLSMLIDSQN NQYILTKPRD STIPRADHHF IKDIVTIGML SLPCGWLCTA IGLPTMFGYI
ICGVLLGPSG LNSIKSIVQV ETLGEFGVFF TLFLVGLEFS PEKLRKVWRI SLQGPCYMTL
LMIAFGLWWG HLLRIRPTQS VFISTCLSLS STPLVSRFLV GSARGDKEAG DIDYSTVLLG
MLVMQDVQLG LFIAVMPTLI QAGAGASSSV VMEVLRILFL IGQILFSLAA VFLLCLVMKT
YLIGPYYRKL HLESKGNKEI LVLGVSAFTF LMLTVTELLD VSMELGCFLA GALVSSQGHM
VTEEIMTYIE PIRDFLAIIF FASIGLHVFP TFVIYELTVL VFLTLSVVIM KFVLAVLVLS
LILPRSSQYI KWIVSAGLAQ VSEFSFVLGS RARRAGILSR EVYLLILSVT TLSLLLAPVL
WKAAITKCVP RPERRSSL
