ID   TMED5_BOVIN             Reviewed;         226 AA.
AC   Q2KJ84; A5D9A9;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Transmembrane emp24 domain-containing protein 5;
DE   AltName: Full=p24 family protein gamma 2;
DE            Short=p24gamma2;
DE   Flags: Precursor;
GN   Name=TMED5;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Potential role in vesicular protein trafficking, mainly in
CC       the early secretory pathway. Required for the maintenance of the Golgi
CC       apparatus; involved in protein exchange between Golgi stacks during
CC       assembly. Probably not required for COPI-vesicle-mediated retrograde
CC       transport (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with TMED9 and TMED10. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus,
CC       cis-Golgi network membrane {ECO:0000250}; Single-pass type I membrane
CC       protein {ECO:0000250}. Endoplasmic reticulum-Golgi intermediate
CC       compartment membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Note=Probably cycles between compartments of the early
CC       secretatory pathway. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR   EMBL; BT030528; ABQ12968.1; -; mRNA.
DR   EMBL; BC105474; AAI05475.1; -; mRNA.
DR   RefSeq; NP_001039842.1; NM_001046377.1.
DR   AlphaFoldDB; Q2KJ84; -.
DR   SMR; Q2KJ84; -.
DR   STRING; 9913.ENSBTAP00000032097; -.
DR   PaxDb; Q2KJ84; -.
DR   PRIDE; Q2KJ84; -.
DR   Ensembl; ENSBTAT00000032159; ENSBTAP00000032097; ENSBTAG00000020180.
DR   GeneID; 534351; -.
DR   KEGG; bta:534351; -.
DR   CTD; 50999; -.
DR   VEuPathDB; HostDB:ENSBTAG00000020180; -.
DR   VGNC; VGNC:35938; TMED5.
DR   eggNOG; KOG3287; Eukaryota.
DR   GeneTree; ENSGT00940000155468; -.
DR   HOGENOM; CLU_066963_0_0_1; -.
DR   InParanoid; Q2KJ84; -.
DR   OrthoDB; 1292519at2759; -.
DR   TreeFam; TF313000; -.
DR   Proteomes; UP000009136; Chromosome 3.
DR   Bgee; ENSBTAG00000020180; Expressed in spiral colon and 108 other tissues.
DR   ExpressionAtlas; Q2KJ84; baseline and differential.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR   GO; GO:0090161; P:Golgi ribbon formation; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   InterPro; IPR036598; GOLD_dom_sf.
DR   InterPro; IPR015717; TMED5.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   PANTHER; PTHR22811:SF41; PTHR22811:SF41; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
DR   SUPFAM; SSF101576; SSF101576; 1.
DR   PROSITE; PS50866; GOLD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Golgi apparatus; Membrane; Protein transport;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..226
FT                   /note="Transmembrane emp24 domain-containing protein 5"
FT                   /id="PRO_0000240347"
FT   TOPO_DOM        25..193
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        215..226
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          42..123
FT                   /note="GOLD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT   MOTIF           217..218
FT                   /note="Mediates export from ER"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   226 AA;  25875 MW;  36E306A97489647E CRC64;
     MGDKTWLPFP VVLLAALLLP RAAGFTPSLD SDFTFTLPAG QKECFYQPMP LKASLEIEYQ
     VLDGAGLDID FHLASPEGKT LVFEQRKSDG VHTIETEVGD YMFCFDNTFS TISEKVIFFE
     LILDNMGEQE QEQEDWKKYI TGTDMLDMKL EDILESINSI KSRLSKSGHI QTLLRAFEAR
     DRNIQESNFD RVNFWSMVNL VVMVVVSAIQ VYMLKSLFED KRKSRT