ID   TMED5_RAT               Reviewed;         229 AA.
AC   Q6AXN3;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Transmembrane emp24 domain-containing protein 5;
DE   AltName: Full=p24 family protein gamma-2;
DE            Short=p24gamma2;
DE   Flags: Precursor;
GN   Name=Tmed5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Potential role in vesicular protein trafficking, mainly in
CC       the early secretory pathway. Required for the maintenance of the Golgi
CC       apparatus; involved in protein exchange between Golgi stacks during
CC       assembly. Probably not required for COPI-vesicle-mediated retrograde
CC       transport (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with TMED9 and TMED10. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus,
CC       cis-Golgi network membrane {ECO:0000250}; Single-pass type I membrane
CC       protein {ECO:0000250}. Endoplasmic reticulum-Golgi intermediate
CC       compartment membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Note=Probably cycles between compartments of the early
CC       secretatory pathway. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR   EMBL; BC079452; AAH79452.1; -; mRNA.
DR   RefSeq; NP_001007620.1; NM_001007619.1.
DR   AlphaFoldDB; Q6AXN3; -.
DR   SMR; Q6AXN3; -.
DR   STRING; 10116.ENSRNOP00000000083; -.
DR   jPOST; Q6AXN3; -.
DR   PaxDb; Q6AXN3; -.
DR   PRIDE; Q6AXN3; -.
DR   Ensembl; ENSRNOT00000119350; ENSRNOP00000084338; ENSRNOG00000000073.
DR   GeneID; 289883; -.
DR   KEGG; rno:289883; -.
DR   UCSC; RGD:1359437; rat.
DR   CTD; 50999; -.
DR   RGD; 1359437; Tmed5.
DR   eggNOG; KOG3287; Eukaryota.
DR   GeneTree; ENSGT00940000155468; -.
DR   HOGENOM; CLU_066963_0_0_1; -.
DR   InParanoid; Q6AXN3; -.
DR   OrthoDB; 1292519at2759; -.
DR   Reactome; R-RNO-3238698; WNT ligand biogenesis and trafficking.
DR   PRO; PR:Q6AXN3; -.
DR   Proteomes; UP000002494; Chromosome 14.
DR   Genevisible; Q6AXN3; RN.
DR   GO; GO:0005801; C:cis-Golgi network; ISS:UniProtKB.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0070971; C:endoplasmic reticulum exit site; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; ISS:UniProtKB.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR   GO; GO:0090161; P:Golgi ribbon formation; ISS:UniProtKB.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   InterPro; IPR036598; GOLD_dom_sf.
DR   InterPro; IPR015717; TMED5.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   PANTHER; PTHR22811:SF41; PTHR22811:SF41; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
DR   SUPFAM; SSF101576; SSF101576; 1.
DR   PROSITE; PS50866; GOLD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Golgi apparatus; Membrane; Protein transport;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..229
FT                   /note="Transmembrane emp24 domain-containing protein 5"
FT                   /id="PRO_0000010392"
FT   TOPO_DOM        28..196
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        218..229
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          45..126
FT                   /note="GOLD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
SQ   SEQUENCE   229 AA;  26136 MW;  2A2D8070B26F6C68 CRC64;
     MGVRMWLPFP MLLLSALPAT LLSGAAGFTP SLDSDFTFTL PAGQKECFYQ PMPLKASLEI
     EYQVLDGGEL DIDFHLASPE GRTLVFEQRK SDGVHTVETE DGDYMFCFDN TFSTISEKVI
     FFELILDNMG EEVEGQEDWK KYITNTDVLE MKLEDILESI NSIKSRLSKS GHIQTLLRAF
     EARDRNIQES NFDRVNFWSV VNLMVMVVVS AIQVYTLKSL FEDKRKSRT