ID   TMEDA_CANGA             Reviewed;         208 AA.
AC   Q6FRZ3;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Endoplasmic reticulum vesicle protein 25;
DE   Flags: Precursor;
GN   Name=ERV25; OrderedLocusNames=CAGL0H04741g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived
CC       transport vesicles. Required for efficient transport of a subset of
CC       secretory proteins to the Golgi. Facilitates retrograde transport from
CC       the Golgi to the endoplasmic reticulum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus
CC       membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Note=Recycles between endoplasmic reticulum and Golgi.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAG59934.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR380954; CAG59934.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_447001.1; XM_447001.1.
DR   AlphaFoldDB; Q6FRZ3; -.
DR   SMR; Q6FRZ3; -.
DR   STRING; 5478.XP_447001.1; -.
DR   EnsemblFungi; CAG59934; CAG59934; CAGL0H04741g.
DR   GeneID; 2888561; -.
DR   KEGG; cgr:CAGL0H04741g; -.
DR   eggNOG; KOG1691; Eukaryota.
DR   HOGENOM; CLU_066963_3_0_1; -.
DR   InParanoid; Q6FRZ3; -.
DR   Proteomes; UP000002428; Chromosome H.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IEA:EnsemblFungi.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEA:EnsemblFungi.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
DR   PROSITE; PS50866; GOLD; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..208
FT                   /note="Endoplasmic reticulum vesicle protein 25"
FT                   /id="PRO_0000237690"
FT   TOPO_DOM        16..177
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        199..208
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          28..118
FT                   /note="GOLD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
SQ   SEQUENCE   208 AA;  24001 MW;  F1801C44DDC2B052 CRC64;
     MKFLLLLLLA PFISALRFDL KAESKPEQMC IRDFVSEGEL VVINIDTDGS LNDGNVLNLY
     VHDSNGNEYR RLKNFVGEQR IAFTAPATTS FDVCFENTLD SNRGNRNAKR AIELDIESGS
     QARDWNKISA TEKLRPIELE LRKIEELTDE IVDELNYLKN REERLRNTNE STNERVRNFS
     VLVIIVLTSL GAWQVNYLKN YFKSKHII