ID   TMEDA_CRYNB             Reviewed;         213 AA.
AC   P0CN73; Q55X80; Q5KMQ3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Endoplasmic reticulum vesicle protein 25;
DE   Flags: Precursor;
GN   Name=ERV25; OrderedLocusNames=CNBB4820;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived
CC       transport vesicles. Required for efficient transport of a subset of
CC       secretory proteins to the Golgi. Facilitates retrograde transport from
CC       the Golgi to the endoplasmic reticulum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus
CC       membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Note=Recycles between endoplasmic reticulum and Golgi.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR   EMBL; AAEY01000011; EAL22307.1; -; Genomic_DNA.
DR   RefSeq; XP_776954.1; XM_771861.1.
DR   AlphaFoldDB; P0CN73; -.
DR   SMR; P0CN73; -.
DR   PRIDE; P0CN73; -.
DR   EnsemblFungi; EAL22307; EAL22307; CNBB4820.
DR   GeneID; 4934576; -.
DR   KEGG; cnb:CNBB4820; -.
DR   VEuPathDB; FungiDB:CNBB4820; -.
DR   HOGENOM; CLU_066963_3_0_1; -.
DR   Proteomes; UP000001435; Chromosome 2.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IEA:EnsemblFungi.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEA:EnsemblFungi.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Protein transport; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..213
FT                   /note="Endoplasmic reticulum vesicle protein 25"
FT                   /id="PRO_0000410091"
FT   TOPO_DOM        21..181
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..213
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          33..122
FT                   /note="GOLD"
SQ   SEQUENCE   213 AA;  24035 MW;  D8E807832BA21173 CRC64;
     MILRIPSLLY LFTLLTAVYA VKFDLTSDRN PKPSIIWNFA SAHSLVIVTA NVPGEPDQQV
     DIQILDGSER GNVYLSKKDV RGEARLAVTT HESADVGVCL TNRYTGSGNP RVVRSVELDV
     DIGADAIDYN AIANQESLSI LEVEMRKLEA VTKEIVEEMG YLQRREMRMR DTNESTNQRV
     KVFSVLIICC TIGLGVWQLL HLRSFFKRKY LID