ID   TMEDA_NEUCR             Reviewed;         216 AA.
AC   Q9HEK4;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 3.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Endoplasmic reticulum vesicle protein 25;
DE   Flags: Precursor;
GN   Name=erv-1; Synonyms=erv25; ORFNames=12F11.230, NCU01342;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived
CC       transport vesicles. Required for efficient transport of a subset of
CC       secretory proteins to the Golgi. Facilitates retrograde transport from
CC       the Golgi to the endoplasmic reticulum (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus
CC       membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Note=Recycles between endoplasmic reticulum and Golgi.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC18232.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL451017; CAC18232.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM002240; EAA32192.3; -; Genomic_DNA.
DR   RefSeq; XP_961428.3; XM_956335.3.
DR   AlphaFoldDB; Q9HEK4; -.
DR   SMR; Q9HEK4; -.
DR   STRING; 5141.EFNCRP00000004098; -.
DR   EnsemblFungi; EAA32192; EAA32192; NCU01342.
DR   GeneID; 3877556; -.
DR   KEGG; ncr:NCU01342; -.
DR   VEuPathDB; FungiDB:NCU01342; -.
DR   HOGENOM; CLU_066963_3_0_1; -.
DR   InParanoid; Q9HEK4; -.
DR   Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
DR   PROSITE; PS50866; GOLD; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..216
FT                   /note="Endoplasmic reticulum vesicle protein 25"
FT                   /id="PRO_0000237696"
FT   TOPO_DOM        21..185
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        207..216
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          36..126
FT                   /note="GOLD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
SQ   SEQUENCE   216 AA;  24613 MW;  B2623B4D889F3E6E CRC64;
     MASLKSLLSG FLLLAGAAQA LKFDLEATSS HHSNQRRCIR NFVNKDTLVV VTATLDGYKG
     DGMNVNMHIS DSHGNEYGKA KDIAGEQRIV FTSHHDAAFD VCFENYLTGS KYVENPRRHV
     ELDIDIGADA KDWSAIQATE KLKPLETDLR RIEELVGEVV NEMDYLRARE QKLRDTNEST
     NNRVKWFGMA TTFLLIALWG WQIMYLRAYF RSKHLI