ID   TMEDE_DROPS             Reviewed;         216 AA.
AC   Q295B2;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Transmembrane emp24 domain-containing protein eca {ECO:0000250|UniProtKB:Q8SXY6};
DE   Flags: Precursor;
GN   Name=eca {ECO:0000250|UniProtKB:Q8SXY6}; ORFNames=GA17284;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1] {ECO:0000312|EMBL:EAL28800.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Eca and bai are essential, though not redundant, for
CC       dorsoventral patterning of the embryo. Specifically required during
CC       early embryogenesis for the activity of maternal tkv, while the zygotic
CC       tkv is not affected. Involved in Golgi organization (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000255};
CC       Single-pass type I membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000255}.
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DR   EMBL; CM000070; EAL28800.1; -; Genomic_DNA.
DR   RefSeq; XP_001359650.1; XM_001359613.4.
DR   AlphaFoldDB; Q295B2; -.
DR   SMR; Q295B2; -.
DR   STRING; 7237.FBpp0281298; -.
DR   EnsemblMetazoa; FBtr0282860; FBpp0281298; FBgn0077297.
DR   GeneID; 4802805; -.
DR   KEGG; dpo:Dpse_GA17284; -.
DR   eggNOG; KOG1690; Eukaryota.
DR   HOGENOM; CLU_066963_2_2_1; -.
DR   InParanoid; Q295B2; -.
DR   OMA; STAIKWS; -.
DR   PhylomeDB; Q295B2; -.
DR   Proteomes; UP000001819; Chromosome 2.
DR   Bgee; FBgn0077297; Expressed in male reproductive system and 3 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009953; P:dorsal/ventral pattern formation; ISS:UniProtKB.
DR   InterPro; IPR015720; Emp24-like.
DR   InterPro; IPR009038; GOLD_dom.
DR   PANTHER; PTHR22811; PTHR22811; 1.
DR   Pfam; PF01105; EMP24_GP25L; 1.
DR   SMART; SM01190; EMP24_GP25L; 1.
DR   PROSITE; PS50866; GOLD; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Developmental protein; Endoplasmic reticulum; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..216
FT                   /note="Transmembrane emp24 domain-containing protein eca"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000393930"
FT   TOPO_DOM        21..182
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        204..216
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          30..126
FT                   /note="GOLD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT   COILED          134..164
FT                   /evidence="ECO:0000255"
FT   MOTIF           213..216
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   216 AA;  25168 MW;  CC23E2B537986BB5 CRC64;
     MRDQFICLAL LLCALHSACG LYFHISETER KCFIEEVPDE TTVIVNYKVE LYDPRSNGFM
     PSSPGIGMHV EVRDSDDKII LSRVYSSQGR ISFTSHTPGE HVICMYSNST AWFSGAQLRV
     HLDIQVGEHA IDYANVAQKE KLTELQLRIR QLLDQVEQIT KEQNYQRYRE ERFRHTSEST
     NSRVLWWSLA QTLVLVCMGF WQMRHLKSFF EAKKLV