ID   TMG10_METJA             Reviewed;         351 AA.
AC   Q58120;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=tRNA (guanine(10)-N2)-dimethyltransferase;
DE            EC=2.1.1.213;
DE   AltName: Full=tRNA:G10 dimethyltransferase;
GN   Name=trmG10; OrderedLocusNames=MJ0710;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Catalyzes the adenosylmethionine-dependent methylation of the
CC       exocyclic amino group (N(2)) of guanosine at position 10 of various
CC       tRNAs. Acts via a two-step process that leads to the formation of
CC       either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G) (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC         N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43124, Rhea:RHEA-COMP:10355, Rhea:RHEA-COMP:10358,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.213;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Trm-G10
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98704.1; -; Genomic_DNA.
DR   PIR; F64388; F64388.
DR   RefSeq; WP_010870216.1; NC_000909.1.
DR   AlphaFoldDB; Q58120; -.
DR   SMR; Q58120; -.
DR   STRING; 243232.MJ_0710; -.
DR   EnsemblBacteria; AAB98704; AAB98704; MJ_0710.
DR   GeneID; 1451578; -.
DR   KEGG; mja:MJ_0710; -.
DR   eggNOG; arCOG00047; Archaea.
DR   HOGENOM; CLU_057819_1_0_2; -.
DR   InParanoid; Q58120; -.
DR   OMA; FFHPGVM; -.
DR   OrthoDB; 47556at2157; -.
DR   PhylomeDB; Q58120; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR004114; THUMP_dom.
DR   InterPro; IPR005885; TrmG10.
DR   Pfam; PF02926; THUMP; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   SMART; SM00981; THUMP; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR01177; TIGR01177; 1.
DR   PROSITE; PS51165; THUMP; 1.
DR   PROSITE; PS01261; UPF0020; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT   CHAIN           1..351
FT                   /note="tRNA (guanine(10)-N2)-dimethyltransferase"
FT                   /id="PRO_0000140481"
FT   DOMAIN          57..165
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
SQ   SEQUENCE   351 AA;  40483 MW;  4D0E17229D924E91 CRC64;
     MIGYVLNGEH EEIPYGELMA LLEIFNYNGS VERLKRYVIT EDSPAKDIVK RSGYIDEGHR
     IIFRYNLEEK SVDLVDKIVN DFINSFKDFV ANIDYPDIDE SKSYAVRVLK LHKDEFTKSI
     DSLRIEKEIG GIIKLKTNAK VNLTKPDILV RVVILENTFF ISNVLEMRDR EYFQKNRPHL
     RKYFHPGCML PKLARAMVNL ARVKEGDIVL DPFCGTGGFL IEAGLIGAKL IGCDIDWRMA
     SGTLINLEEY NLLDKVIKVK RLDAKYVKEF LNELNIEKVD AIVTDPPYGI STAKKGEIEK
     ILETLPEVIK DNGYFVFAYP KKIELDMELE GLYKVYIHKG LIRHIHVYKK I