ID   TMG10_METTH             Reviewed;         336 AA.
AC   O26820;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=tRNA (guanine(10)-N2)-dimethyltransferase;
DE            EC=2.1.1.213;
DE   AltName: Full=tRNA:G10 dimethyltransferase;
GN   Name=trmG10; OrderedLocusNames=MTH_724;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the adenosylmethionine-dependent methylation of the
CC       exocyclic amino group (N(2)) of guanosine at position 10 of various
CC       tRNAs. Acts via a two-step process that leads to the formation of
CC       either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G) (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC         N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43124, Rhea:RHEA-COMP:10355, Rhea:RHEA-COMP:10358,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.213;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Trm-G10
CC       family. {ECO:0000305}.
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DR   EMBL; AE000666; AAB85229.1; -; Genomic_DNA.
DR   PIR; G69196; G69196.
DR   RefSeq; WP_010876363.1; NC_000916.1.
DR   AlphaFoldDB; O26820; -.
DR   SMR; O26820; -.
DR   STRING; 187420.MTH_724; -.
DR   EnsemblBacteria; AAB85229; AAB85229; MTH_724.
DR   GeneID; 1470685; -.
DR   KEGG; mth:MTH_724; -.
DR   PATRIC; fig|187420.15.peg.709; -.
DR   HOGENOM; CLU_057819_1_0_2; -.
DR   OMA; FFHPGVM; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR004114; THUMP_dom.
DR   InterPro; IPR005885; TrmG10.
DR   Pfam; PF02926; THUMP; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   SMART; SM00981; THUMP; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR01177; TIGR01177; 1.
DR   PROSITE; PS51165; THUMP; 1.
DR   PROSITE; PS01261; UPF0020; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT   CHAIN           1..336
FT                   /note="tRNA (guanine(10)-N2)-dimethyltransferase"
FT                   /id="PRO_0000140482"
FT   DOMAIN          50..147
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
SQ   SEQUENCE   336 AA;  38020 MW;  D305B3FFADE1F5CC CRC64;
     MEMMVLLSQE HPELPSAELR SVLMSEGIDF SVIESGSGYE VIDAPRSTWK ILKKRLAYAH
     EISEVIGYAA ADDLDDAAEE IDWSKYVRES FAVRIRKLCG DVDSRTLERT IGAIIKEDTG
     LKVDLEKPWT LIRPVLINDR FILTRRLAVI SKEHFNQARP HKRPFFYPGS MSPKLARCMV
     NLSGVKAGDR ILDPFCGTGG ILIEAGLMGV RVVGADIDWR MVEGTRENLQ HYGITDFEVI
     RSDARDLRLD EKVDAIVTDP PYGISASTAG EKSEKLYREF LDSAHSNLAE GGMICMAAPH
     YLDLESLIDE RFSIRERYSM RMHRSLTRVI RVIERV