TMG3_HUMAN
ID TMG3_HUMAN Reviewed; 231 AA.
AC Q9BZD7; A1A523; A1A575; Q8N2N6;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Transmembrane gamma-carboxyglutamic acid protein 3;
DE AltName: Full=Proline-rich gamma-carboxyglutamic acid protein 3;
DE Short=Proline-rich Gla protein 3;
DE Flags: Precursor;
GN Name=PRRG3; Synonyms=PRGP3, TMG3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-153, AND TISSUE SPECIFICITY.
RC TISSUE=Spinal cord;
RX PubMed=11171957; DOI=10.1073/pnas.98.4.1370;
RA Kulman J.D., Harris J.E., Xie L., Davie E.W.;
RT "Identification of two novel transmembrane gamma-carboxyglutamic acid
RT proteins expressed broadly in fetal and adult tissues.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:1370-1375(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-153.
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT SER-153.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-153.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in brain, lung, kidney and heart.
CC {ECO:0000269|PubMed:11171957}.
CC -!- PTM: Gla residues are produced after subsequent post-translational
CC modifications of glutamate by a vitamin K-dependent gamma-carboxylase.
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DR EMBL; AF326350; AAK00955.1; -; mRNA.
DR EMBL; AK074574; BAC11069.1; -; mRNA.
DR EMBL; CH471169; EAW99407.1; -; Genomic_DNA.
DR EMBL; BC128256; AAI28257.1; -; mRNA.
DR EMBL; BC128409; AAI28410.1; -; mRNA.
DR CCDS; CCDS14699.1; -.
DR RefSeq; NP_076987.3; NM_024082.3.
DR RefSeq; XP_005274804.1; XM_005274747.3.
DR RefSeq; XP_011529499.1; XM_011531197.2.
DR AlphaFoldDB; Q9BZD7; -.
DR SMR; Q9BZD7; -.
DR BioGRID; 122515; 12.
DR IntAct; Q9BZD7; 4.
DR STRING; 9606.ENSP00000359378; -.
DR iPTMnet; Q9BZD7; -.
DR PhosphoSitePlus; Q9BZD7; -.
DR BioMuta; PRRG3; -.
DR DMDM; 209572687; -.
DR MassIVE; Q9BZD7; -.
DR PaxDb; Q9BZD7; -.
DR PeptideAtlas; Q9BZD7; -.
DR PRIDE; Q9BZD7; -.
DR Antibodypedia; 30695; 131 antibodies from 21 providers.
DR DNASU; 79057; -.
DR Ensembl; ENST00000370353.3; ENSP00000359378.3; ENSG00000130032.17.
DR Ensembl; ENST00000538575.5; ENSP00000440217.1; ENSG00000130032.17.
DR Ensembl; ENST00000674457.1; ENSP00000501544.1; ENSG00000130032.17.
DR GeneID; 79057; -.
DR KEGG; hsa:79057; -.
DR MANE-Select; ENST00000674457.1; ENSP00000501544.1; NM_001372163.1; NP_001359092.1.
DR UCSC; uc004few.3; human.
DR CTD; 79057; -.
DR GeneCards; PRRG3; -.
DR HGNC; HGNC:30798; PRRG3.
DR HPA; ENSG00000130032; Tissue enhanced (brain, epididymis).
DR MIM; 300685; gene.
DR neXtProt; NX_Q9BZD7; -.
DR OpenTargets; ENSG00000130032; -.
DR PharmGKB; PA134963933; -.
DR VEuPathDB; HostDB:ENSG00000130032; -.
DR eggNOG; ENOG502RXJN; Eukaryota.
DR GeneTree; ENSGT00940000160946; -.
DR HOGENOM; CLU_103591_0_0_1; -.
DR InParanoid; Q9BZD7; -.
DR OMA; TVYMVVP; -.
DR OrthoDB; 1305760at2759; -.
DR PhylomeDB; Q9BZD7; -.
DR TreeFam; TF332123; -.
DR PathwayCommons; Q9BZD7; -.
DR SignaLink; Q9BZD7; -.
DR BioGRID-ORCS; 79057; 9 hits in 688 CRISPR screens.
DR GenomeRNAi; 79057; -.
DR Pharos; Q9BZD7; Tbio.
DR PRO; PR:Q9BZD7; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q9BZD7; protein.
DR Bgee; ENSG00000130032; Expressed in sural nerve and 128 other tissues.
DR ExpressionAtlas; Q9BZD7; baseline and differential.
DR Genevisible; Q9BZD7; HS.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR Gene3D; 4.10.740.10; -; 1.
DR InterPro; IPR017857; Coagulation_fac-like_Gla_dom.
DR InterPro; IPR035972; GLA-like_dom_SF.
DR InterPro; IPR000294; GLA_domain.
DR Pfam; PF00594; Gla; 1.
DR PRINTS; PR00001; GLABLOOD.
DR SMART; SM00069; GLA; 1.
DR SUPFAM; SSF57630; SSF57630; 1.
DR PROSITE; PS00011; GLA_1; 1.
DR PROSITE; PS50998; GLA_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Gamma-carboxyglutamic acid; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT PROPEP 1..19
FT /evidence="ECO:0000255"
FT /id="PRO_0000022547"
FT CHAIN 20..231
FT /note="Transmembrane gamma-carboxyglutamic acid protein 3"
FT /id="PRO_0000022548"
FT TOPO_DOM 20..78
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 20..65
FT /note="Gla"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT REGION 140..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 25
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 26
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 33
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 35
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 38
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 39
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 44
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 45
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 48
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 51
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 54
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT MOD_RES 58
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT DISULFID 36..41
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00463"
FT VARIANT 153
FT /note="N -> S (in dbSNP:rs4323608)"
FT /evidence="ECO:0000269|PubMed:11171957,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.3"
FT /id="VAR_046712"
FT CONFLICT 189
FT /note="T -> P (in Ref. 2; BAC11069)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 231 AA; 25875 MW; B012F1A2784917B2 CRC64;
MAVFLEAKDA HSVLKRFPRA NEFLEELRQG TIERECMEEI CSYEEVKEVF ENKEKTMEFW
KGYPNAVYSV RDPSQSSDAM YVVVPLLGVA LLIVIALFII WRCQLQKATR HHPSYAQNRY
LASRAGHTLP RVMVYRGTVH SQGEPSGHRE AANSPQVVLG PSRGGRTTVR LESTLYLPEL
SLSRLSSTTP PPSYEEVTAP QESSSEEASV SYSDPPPKYE EIVAANPGAD K
