BTBD7_MOUSE
ID BTBD7_MOUSE Reviewed; 1130 AA.
AC Q8CFE5; Q80TC3; Q8BZY9;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=BTB/POZ domain-containing protein 7;
DE AltName: Full=Function-unkown protein 1;
GN Name=Btbd7; Synonyms=Fup1, Kiaa1525;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=11527404; DOI=10.1006/bbrc.2001.5478;
RA Pan W., Zhang Q., Xi Q.S., Gan R.B., Li T.P.;
RT "FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3
RT cell proliferation and tumor formation in nude mice.";
RL Biochem. Biophys. Res. Commun. 286:1033-1038(2001).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=20671187; DOI=10.1126/science.1191880;
RA Onodera T., Sakai T., Hsu J.C., Matsumoto K., Chiorini J.A., Yamada K.M.;
RT "Btbd7 regulates epithelial cell dynamics and branching morphogenesis.";
RL Science 329:562-565(2010).
CC -!- FUNCTION: Acts as a mediator of epithelial dynamics and organ branching
CC by promoting cleft progression. Induced following accumulation of
CC fibronectin in forming clefts, leading to local expression of the cell-
CC scattering SNAIL2 and suppression of E-cadherin levels, thereby
CC altering cell morphology and reducing cell-cell adhesion. This
CC stimulates cell separation at the base of forming clefts by local,
CC dynamic intercellular gap formation and promotes cleft progression.
CC {ECO:0000269|PubMed:20671187}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11527404}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CFE5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CFE5-2; Sequence=VSP_013828, VSP_013829;
CC Name=3;
CC IsoId=Q8CFE5-3; Sequence=VSP_013830, VSP_013831;
CC -!- TISSUE SPECIFICITY: Specifically expressed in embryonic epithelia.
CC {ECO:0000269|PubMed:20671187}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in developing salivary glands at
CC 13 dpc, a stage of particularly active salivary gland branching.
CC Concentrated around the bottom and lower sides of forming clefts, while
CC it is weakly or not expressed in other salivary epithelial regions.
CC Also expressed in mesenchyme containing high levels of fibronectin.
CC -!- INDUCTION: By fibronectin. {ECO:0000269|PubMed:20671187}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65804.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK033160; BAC28177.1; -; mRNA.
DR EMBL; AK122522; BAC65804.1; ALT_INIT; mRNA.
DR EMBL; BC041669; AAH41669.1; -; mRNA.
DR EMBL; BC057303; AAH57303.1; -; mRNA.
DR CCDS; CCDS26125.1; -. [Q8CFE5-1]
DR RefSeq; NP_766394.2; NM_172806.2. [Q8CFE5-1]
DR RefSeq; XP_006515918.1; XM_006515855.3.
DR AlphaFoldDB; Q8CFE5; -.
DR STRING; 10090.ENSMUSP00000046951; -.
DR iPTMnet; Q8CFE5; -.
DR PhosphoSitePlus; Q8CFE5; -.
DR EPD; Q8CFE5; -.
DR PaxDb; Q8CFE5; -.
DR PRIDE; Q8CFE5; -.
DR ProteomicsDB; 273560; -. [Q8CFE5-1]
DR ProteomicsDB; 273561; -. [Q8CFE5-2]
DR ProteomicsDB; 273562; -. [Q8CFE5-3]
DR Antibodypedia; 55719; 112 antibodies from 26 providers.
DR Ensembl; ENSMUST00000045652; ENSMUSP00000046951; ENSMUSG00000041702. [Q8CFE5-1]
DR Ensembl; ENSMUST00000223554; ENSMUSP00000152426; ENSMUSG00000041702. [Q8CFE5-1]
DR GeneID; 238386; -.
DR KEGG; mmu:238386; -.
DR UCSC; uc007ouq.1; mouse. [Q8CFE5-1]
DR UCSC; uc007ous.1; mouse. [Q8CFE5-3]
DR UCSC; uc007ouu.1; mouse. [Q8CFE5-2]
DR CTD; 55727; -.
DR MGI; MGI:1917858; Btbd7.
DR VEuPathDB; HostDB:ENSMUSG00000041702; -.
DR eggNOG; KOG2838; Eukaryota.
DR GeneTree; ENSGT00390000014092; -.
DR HOGENOM; CLU_007289_0_0_1; -.
DR InParanoid; Q8CFE5; -.
DR OMA; QPSWQAD; -.
DR OrthoDB; 73978at2759; -.
DR PhylomeDB; Q8CFE5; -.
DR TreeFam; TF316112; -.
DR BioGRID-ORCS; 238386; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Btbd7; mouse.
DR PRO; PR:Q8CFE5; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8CFE5; protein.
DR Bgee; ENSMUSG00000041702; Expressed in otolith organ and 220 other tissues.
DR Genevisible; Q8CFE5; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0061138; P:morphogenesis of a branching epithelium; IEA:InterPro.
DR GO; GO:0060693; P:regulation of branching involved in salivary gland morphogenesis; IMP:MGI.
DR Gene3D; 3.30.710.10; -; 2.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR042345; Btbd7.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR16064; PTHR16064; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 2.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 2.
DR SUPFAM; SSF54695; SSF54695; 2.
DR PROSITE; PS50097; BTB; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Lipoprotein; Myristate;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..1130
FT /note="BTB/POZ domain-containing protein 7"
FT /id="PRO_0000186216"
FT DOMAIN 142..211
FT /note="BTB 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 247..341
FT /note="BTB 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 413..479
FT /note="BACK"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 898..1050
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1062..1130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 898..918
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 919..937
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 979..993
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 996..1025
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1062..1078
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1079..1122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 722
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P203"
FT MOD_RES 1008
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P203"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 389..395
FT /note="CEDIIAE -> MNSICLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_013828"
FT VAR_SEQ 397..1130
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_013829"
FT VAR_SEQ 537..565
FT /note="MKRGLISTPPSDMLPTAEGGKSNAWLRQK -> VSSFLLLFLSRKEKCQTYP
FT ALIILNNFSY (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_013830"
FT VAR_SEQ 566..1130
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_013831"
SQ SEQUENCE 1130 AA; 126243 MW; 92390ACAD17C6A5E CRC64;
MGANASNYPH SCSPRVGGNS QAQQTFIGTS SYSQQGYGCE SKLYSLDHGH EKPQDKKKRT
SGLATLKKKF IKRRKSNRSA DHAKQMRELL SGWDVRDVNA LVEEYEGTSA LKELSLQASL
ARPEARTLQK DMADLYEDKY CTDVDLIFQE TCFPVHRAIL AARCPFFKTL LSSSPEYGAE
IIMDISTAGI DMPMFSALLH YLYTGEFGME DSRFQNVDIL VQLSEEFGTP NPLDVDMRGL
FDYMCYYDVV LSFSSDSELV EAFGGNQNCL DEELKAHKAI ISARSPFFRN LLQRRIRTGE
EITDRTLRTP TRIILDESII PKKYAKVILH CMYTDVVDLS VLHCSPSVGS LSEVQALVAG
KPNMTRAEEA MELYHIALFL EFNMLAQGCE DIIAESISLD TLIAVLKWSS HPYGSKWVHR
QAVHFLCEEL SQVMTSDVFY ELSKDHLLTA IQSDYLQASE QDILKYLIKW GEHQLMKRIA
DREPNLLSGT AHSVNKRGVK RRDLDIEELR EILSSLLPFV RIEHILPINS EVLSDAMKRG
LISTPPSDML PTAEGGKSNA WLRQKNAGIY VRPRLFSPYV EEAKSVLDEM MVEQTDLVRL
RMVRMSNVPD TLYMVSNAMP QCCHMISHQQ ISSNQSSPPS VVANEIPVPR LLIMKDMVRR
LQELRHTEQV QRAYALNCGE GATVSYEIQI RVLREFGLAD AAAELLQNPH KFFPDERFGD
ESPLLTMRQP GRCRVNSTPT AETMFTDLDS FVAFHPPLPP PPPPYHPPAT PIHNQLKAGW
KQRPPSHHPS RSFSYPCNHS LFHCRTAPKP GPPPVYLPGV KVAPPDCTNT TGLGRQTVAA
AAAAAAASAA IIPEKQVCPQ PVLNDLMPDI AMGVSTLSLK DRRLPELAAD TELCQTVSEA
GTGPPQHLSC IPQRHTNTSR KKPTLEQKAD GRENQQEYPD LYDFSNAACR PSTPAPGRHS
PSPAHGRYFG PDLYSHNKAS PNGLKSVYLP GQTSPKKQED PRREYPPSPD GHPHRQKREP
IRLDVVEQPP QRPDFPSAAS ENASHGPAHV RARTAVETDL TFGLTSNRPP SHSACSSEVL
EERSSRRLTD SEPLGHGAHQ RNADLERGDS ISRGRRSPSK PDFLYKKSAL
