TMM33_RAT
ID TMM33_RAT Reviewed; 247 AA.
AC Q9Z142;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transmembrane protein 33;
DE AltName: Full=Protein DB83;
GN Name=Tmem33; Synonyms=Db83;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=9872456; DOI=10.1093/dnares/5.5.315;
RA Nakadai T., Kishimoto T., Kokura K., Ohkawa N., Makino Y., Muramatsu M.,
RA Tamura T.;
RT "Cloning of a novel rat gene, DB83, that encodes a putative membrane
RT protein.";
RL DNA Res. 5:315-317(1998).
CC -!- FUNCTION: Acts as a regulator of the tubular endoplasmic reticulum (ER)
CC network by modulating intracellular calcium homeostasis.
CC Mechanistically, stimulates PKD2 calcium-dependent activity (By
CC similarity). Suppresses the RTN3/4-induced formation of the ER tubules.
CC Positively regulates PERK-mediated and IRE1-mediated unfolded protein
CC response signaling. Plays an essential role in VEGF-mediated release of
CC Ca(2+) from ER stores during angiogenesis. Also plays a role in the
CC modulation of innate immune signaling through the cGAS-STING pathway by
CC interacting with RNF26. Participates in lipid metabolism by acting as a
CC downstream effector of the pyruvate kinase/PKM. Forms a complex with
CC RNF5 to facilitate polyubiquitination and subsequent degradation of
CC SCAP on the ER membrane (By similarity). {ECO:0000250|UniProtKB:P57088,
CC ECO:0000250|UniProtKB:Q9CR67}.
CC -!- SUBUNIT: Interacts with EIF2AK3. Interacts with ARL6IP1, isoform RTN1-A
CC of RTN1, isoform RTN2-B of RTN2, isoform 3 of RTN3 and isoform 3 of
CC RTN4. Interacts with RNF5. Interacts with RNF26 (By similarity).
CC Interacts with PKD2 (By similarity). {ECO:0000250|UniProtKB:P57088,
CC ECO:0000250|UniProtKB:Q9CR67}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P57088}; Multi-pass membrane protein
CC {ECO:0000255}. Melanosome {ECO:0000250|UniProtKB:P57088}. Nucleus
CC envelope {ECO:0000250|UniProtKB:P57088}. Note=Co-localizes with RTN4 at
CC the ER sheets. {ECO:0000250|UniProtKB:P57088}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the liver and significantly in
CC brain, lungs and kidneys.
CC -!- SIMILARITY: Belongs to the PER33/POM33 family. {ECO:0000305}.
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DR EMBL; AB006135; BAA75068.1; -; mRNA.
DR PIR; JE0307; JE0307.
DR RefSeq; NP_067703.1; NM_021671.3.
DR AlphaFoldDB; Q9Z142; -.
DR BioGRID; 248752; 1.
DR IntAct; Q9Z142; 2.
DR MINT; Q9Z142; -.
DR STRING; 10116.ENSRNOP00000003128; -.
DR jPOST; Q9Z142; -.
DR PaxDb; Q9Z142; -.
DR PRIDE; Q9Z142; -.
DR Ensembl; ENSRNOT00000110302; ENSRNOP00000097007; ENSRNOG00000002254.
DR GeneID; 59303; -.
DR KEGG; rno:59303; -.
DR UCSC; RGD:708371; rat.
DR CTD; 55161; -.
DR RGD; 708371; Tmem33.
DR eggNOG; KOG4002; Eukaryota.
DR GeneTree; ENSGT00390000011368; -.
DR HOGENOM; CLU_071391_0_0_1; -.
DR InParanoid; Q9Z142; -.
DR OrthoDB; 1360050at2759; -.
DR PhylomeDB; Q9Z142; -.
DR TreeFam; TF314068; -.
DR PRO; PR:Q9Z142; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Genevisible; Q9Z142; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0042470; C:melanosome; ISO:RGD.
DR GO; GO:0005635; C:nuclear envelope; ISO:RGD.
DR GO; GO:0071786; P:endoplasmic reticulum tubular network organization; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0061024; P:membrane organization; IBA:GO_Central.
DR GO; GO:1903896; P:positive regulation of IRE1-mediated unfolded protein response; ISS:UniProtKB.
DR GO; GO:1903899; P:positive regulation of PERK-mediated unfolded protein response; ISS:UniProtKB.
DR GO; GO:1903371; P:regulation of endoplasmic reticulum tubular network organization; ISS:UniProtKB.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; ISS:UniProtKB.
DR InterPro; IPR005344; TMEM33/Pom33.
DR Pfam; PF03661; TMEM33_Pom33; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Endoplasmic reticulum; Immunity; Innate immunity; Membrane;
KW Nucleus; Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P57088"
FT CHAIN 2..247
FT /note="Transmembrane protein 33"
FT /id="PRO_0000220901"
FT TOPO_DOM 2..31
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..100
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..155
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P57088"
SQ SEQUENCE 247 AA; 27983 MW; 0D07BE5911CAD6AE CRC64;
MADTTPNGPQ GAGAVQFMMT NKLDTAMWLS RLFTVYCSAL FVLPLLGLHE AASFYQRALL
ANALTSALRL HQRLPHFQLS RAFLAQALLE DSCHYLLYSL IFVNSYPVTM SIFPVLLFSL
LHAATYTKKV LDAKGSNSLP LLRSVLDKLS TNQQNILKFI ACNEIFLMPA TVFMLFSGQG
SLLQPFIYYR FLTLRYSSRR NPYCRNLFNE LRIVVEHIIM KPSCPLFVRR LCLQSIAFIS
RLAPTVA
