ID   TMM65_BOVIN             Reviewed;         236 AA.
AC   Q0VCH8;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Transmembrane protein 65;
DE   Flags: Precursor;
GN   Name=TMEM65;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Placenta;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play an important role in cardiac development and
CC       function. May regulate cardiac conduction and the function of the gap
CC       junction protein GJA1. May contribute to the stability and proper
CC       localization of GJA1 to cardiac intercalated disk thereby regulating
CC       gap junction communication (By similarity). Regulates mitochondrial
CC       respiration and mitochondrial DNA copy number maintenance (By
CC       similarity). {ECO:0000250|UniProtKB:Q4VAE3,
CC       ECO:0000250|UniProtKB:Q6PI78}.
CC   -!- SUBUNIT: Monomer. Homodimer. Interacts with GJA1. Interacts weakly with
CC       DSP. {ECO:0000250|UniProtKB:Q4VAE3}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q4VAE3};
CC       Multi-pass membrane protein {ECO:0000255}. Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q6PI78}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Localizes at the intercalated disk in ventricular
CC       tissue and cardiomyocytes. {ECO:0000250|UniProtKB:Q4VAE3}.
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DR   EMBL; BC120160; AAI20161.1; -; mRNA.
DR   RefSeq; NP_001069786.1; NM_001076318.1.
DR   AlphaFoldDB; Q0VCH8; -.
DR   STRING; 9913.ENSBTAP00000029809; -.
DR   PaxDb; Q0VCH8; -.
DR   PRIDE; Q0VCH8; -.
DR   Ensembl; ENSBTAT00000029813; ENSBTAP00000029809; ENSBTAG00000022114.
DR   GeneID; 614243; -.
DR   KEGG; bta:614243; -.
DR   CTD; 157378; -.
DR   VEuPathDB; HostDB:ENSBTAG00000022114; -.
DR   VGNC; VGNC:36106; TMEM65.
DR   eggNOG; KOG4619; Eukaryota.
DR   GeneTree; ENSGT00390000017802; -.
DR   HOGENOM; CLU_075402_1_0_1; -.
DR   InParanoid; Q0VCH8; -.
DR   OMA; QRCNLGT; -.
DR   OrthoDB; 936872at2759; -.
DR   TreeFam; TF105823; -.
DR   Proteomes; UP000009136; Chromosome 14.
DR   Bgee; ENSBTAG00000022114; Expressed in rectus femoris and 108 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0014704; C:intercalated disc; IEA:Ensembl.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003231; P:cardiac ventricle development; IBA:GO_Central.
DR   GO; GO:1903779; P:regulation of cardiac conduction; IBA:GO_Central.
DR   InterPro; IPR019537; TMEM65.
DR   PANTHER; PTHR21706; PTHR21706; 1.
DR   Pfam; PF10507; TMEM65; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..57
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           58..236
FT                   /note="Transmembrane protein 65"
FT                   /id="PRO_0000251403"
FT   TOPO_DOM        58..106
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        128..138
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT   TRANSMEM        139..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        162..205
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        227..236
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PI78"
SQ   SEQUENCE   236 AA;  25041 MW;  9FC2752B9D8BA386 CRC64;
     MSRLLPLLRS RTARSLRPGP AAAAAPRPPS WCCCGRGLLA LAAPGGPRAL GTHPKKEPIE
     ALNTAQGARD FIYSLHSSER SCLLKELHRF ESIAIAQEKL EAQPPTPGQL RYVFIHNAIP
     FIGFGFLDNA IMIVAGTHIE LSIGIILGIS TMAAAALGNL VSDLAGLGLA GYVEALASRL
     GLSIPDLSPK QVDMWQTRVS SHLGKAVGVT IGCILGMFPL IFFGGGEDDE KLEKKN