TMM65_MOUSE
ID TMM65_MOUSE Reviewed; 234 AA.
AC Q4VAE3; Q810S8;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Transmembrane protein 65;
DE Flags: Precursor;
GN Name=Tmem65;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INTERACTION WITH GJA1 AND DSP.
RX PubMed=26403541; DOI=10.1038/ncomms9391;
RA Sharma P., Abbasi C., Lazic S., Teng A.C., Wang D., Dubois N.,
RA Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C.,
RA Zimmermann W.H., Hamilton R., Sun Y., Liu P.P., Keller G., Stagljar I.,
RA Scott I.C., Kislinger T., Gramolini A.O.;
RT "Evolutionarily conserved intercalated disc protein Tmem65 regulates
RT cardiac conduction and connexin 43 function.";
RL Nat. Commun. 6:8391-8391(2015).
CC -!- FUNCTION: May play an important role in cardiac development and
CC function. May regulate cardiac conduction and the function of the gap
CC junction protein GJA1. May contribute to the stability and proper
CC localization of GJA1 to cardiac intercalated disk thereby regulating
CC gap junction communication (PubMed:26403541). Regulates mitochondrial
CC respiration and mitochondrial DNA copy number maintenance (By
CC similarity). {ECO:0000250|UniProtKB:Q6PI78,
CC ECO:0000269|PubMed:26403541}.
CC -!- SUBUNIT: Monomer. Homodimer. Interacts with GJA1. Interacts weakly with
CC DSP. {ECO:0000269|PubMed:26403541}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26403541};
CC Multi-pass membrane protein {ECO:0000255}. Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q6PI78}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes at the intercalated disk in ventricular
CC tissue and cardiomyocytes. {ECO:0000269|PubMed:26403541}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the ventricular tissue
CC (at protein level). {ECO:0000269|PubMed:26403541}.
CC -!- DEVELOPMENTAL STAGE: Low level expression seen in the heart between 8.5
CC dpc and 15.5 dpc. Expression levels increase progressively after birth,
CC with the highest levels seen in adults. {ECO:0000269|PubMed:26403541}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH49380.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BC049380; AAH49380.1; ALT_INIT; mRNA.
DR EMBL; BC096426; AAH96426.1; -; mRNA.
DR CCDS; CCDS27493.1; -.
DR RefSeq; NP_780421.2; NM_175212.4.
DR AlphaFoldDB; Q4VAE3; -.
DR BioGRID; 217041; 3.
DR IntAct; Q4VAE3; 2.
DR STRING; 10090.ENSMUSP00000071984; -.
DR SwissPalm; Q4VAE3; -.
DR EPD; Q4VAE3; -.
DR jPOST; Q4VAE3; -.
DR MaxQB; Q4VAE3; -.
DR PaxDb; Q4VAE3; -.
DR PeptideAtlas; Q4VAE3; -.
DR PRIDE; Q4VAE3; -.
DR ProteomicsDB; 259133; -.
DR Antibodypedia; 13901; 100 antibodies from 17 providers.
DR Ensembl; ENSMUST00000072113; ENSMUSP00000071984; ENSMUSG00000062373.
DR GeneID; 74868; -.
DR KEGG; mmu:74868; -.
DR UCSC; uc007vto.1; mouse.
DR CTD; 157378; -.
DR MGI; MGI:1922118; Tmem65.
DR VEuPathDB; HostDB:ENSMUSG00000062373; -.
DR eggNOG; KOG4619; Eukaryota.
DR GeneTree; ENSGT00390000017802; -.
DR HOGENOM; CLU_075402_1_0_1; -.
DR InParanoid; Q4VAE3; -.
DR OMA; QRCNLGT; -.
DR OrthoDB; 936872at2759; -.
DR PhylomeDB; Q4VAE3; -.
DR TreeFam; TF105823; -.
DR BioGRID-ORCS; 74868; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Tmem65; mouse.
DR PRO; PR:Q4VAE3; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q4VAE3; protein.
DR Bgee; ENSMUSG00000062373; Expressed in extra-ocular muscle and 220 other tissues.
DR Genevisible; Q4VAE3; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0014704; C:intercalated disc; IDA:UniProtKB.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0003231; P:cardiac ventricle development; IMP:UniProtKB.
DR GO; GO:1903779; P:regulation of cardiac conduction; IMP:UniProtKB.
DR InterPro; IPR019537; TMEM65.
DR PANTHER; PTHR21706; PTHR21706; 1.
DR Pfam; PF10507; TMEM65; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..234
FT /note="Transmembrane protein 65"
FT /id="PRO_0000251405"
FT TOPO_DOM 56..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..138
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q6PI78"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..234
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q6PI78"
SQ SEQUENCE 234 AA; 24918 MW; 619D47EDE37C1396 CRC64;
MSRLLPLLGS RTARSLRPGP AAAPRLPSWC CCGRGLLALG VPGGPRLLGT HPKKEPMEAL
NTAQGARDFI YSLHSTERSC LLKELHRFES IAIAQEKLEA LPPTPGQLRY VFFHNAIPFV
GFGFLDNAIM IVAGTQIELS IGIILGISTM AAAALGNLVS DLAGLGLAGY VEALASRLGL
SIPDLTPKQV DMWQTRVSTH LGKAVGVTIG CILGMFPLIF FGGSEEDEKL ETTN
