TMM74_HUMAN
ID TMM74_HUMAN Reviewed; 305 AA.
AC Q96NL1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transmembrane protein 74;
GN Name=TMEM74;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18294959; DOI=10.1016/j.bbrc.2008.02.055;
RA Yu C., Wang L., Lv B., Lu Y., Zeng L., Chen Y., Ma D., Shi T., Wang L.;
RT "TMEM74, a lysosome and autophagosome protein, regulates autophagy.";
RL Biochem. Biophys. Res. Commun. 369:622-629(2008).
RN [4]
RP FUNCTION.
RX PubMed=19029833; DOI=10.4161/auto.5.1.7247;
RA He P., Peng Z., Luo Y., Wang L., Yu P., Deng W., An Y., Shi T., Ma D.;
RT "High-throughput functional screening for autophagy-related genes and
RT identification of TM9SF1 as an autophagosome-inducing gene.";
RL Autophagy 5:52-60(2009).
CC -!- FUNCTION: Plays an essential role in autophagy. TMEM74-induced
CC autophagy may involve PI3K signal transduction.
CC {ECO:0000269|PubMed:18294959, ECO:0000269|PubMed:19029833}.
CC -!- INTERACTION:
CC Q96NL1; Q13520: AQP6; NbExp=3; IntAct=EBI-10292091, EBI-13059134;
CC Q96NL1; Q969F0: FATE1; NbExp=3; IntAct=EBI-10292091, EBI-743099;
CC Q96NL1; Q9H400: LIME1; NbExp=3; IntAct=EBI-10292091, EBI-2830566;
CC Q96NL1; Q5J8X5: MS4A13; NbExp=3; IntAct=EBI-10292091, EBI-12070086;
CC Q96NL1; Q69YG0: TMEM42; NbExp=3; IntAct=EBI-10292091, EBI-12038591;
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:18294959};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18294959}. Cytoplasmic
CC vesicle, autophagosome membrane {ECO:0000269|PubMed:18294959}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:18294959}.
CC -!- TISSUE SPECIFICITY: Expressed in heart, lung, and placenta.
CC {ECO:0000269|PubMed:18294959}.
CC -!- SIMILARITY: Belongs to the TMEM74 family. {ECO:0000305}.
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DR EMBL; AK055230; BAB70881.1; -; mRNA.
DR EMBL; BC030710; AAH30710.1; -; mRNA.
DR CCDS; CCDS6310.1; -.
DR RefSeq; NP_694560.1; NM_153015.2.
DR AlphaFoldDB; Q96NL1; -.
DR BioGRID; 127621; 124.
DR IntAct; Q96NL1; 5.
DR STRING; 9606.ENSP00000297459; -.
DR TCDB; 8.A.122.2.1; the vesicular membrane neurensin/tmem74 (neurensin/tmem74) family.
DR PhosphoSitePlus; Q96NL1; -.
DR BioMuta; TMEM74; -.
DR DMDM; 74732587; -.
DR MassIVE; Q96NL1; -.
DR PaxDb; Q96NL1; -.
DR PeptideAtlas; Q96NL1; -.
DR PRIDE; Q96NL1; -.
DR ProteomicsDB; 77529; -.
DR Antibodypedia; 3040; 87 antibodies from 22 providers.
DR DNASU; 157753; -.
DR Ensembl; ENST00000297459.4; ENSP00000297459.3; ENSG00000164841.5.
DR GeneID; 157753; -.
DR KEGG; hsa:157753; -.
DR MANE-Select; ENST00000297459.4; ENSP00000297459.3; NM_153015.3; NP_694560.1.
DR UCSC; uc003ymy.2; human.
DR CTD; 157753; -.
DR DisGeNET; 157753; -.
DR GeneCards; TMEM74; -.
DR HGNC; HGNC:26409; TMEM74.
DR HPA; ENSG00000164841; Tissue enhanced (brain).
DR MIM; 613935; gene.
DR neXtProt; NX_Q96NL1; -.
DR OpenTargets; ENSG00000164841; -.
DR PharmGKB; PA142670785; -.
DR VEuPathDB; HostDB:ENSG00000164841; -.
DR eggNOG; ENOG502QUYY; Eukaryota.
DR GeneTree; ENSGT00530000063880; -.
DR HOGENOM; CLU_079915_0_0_1; -.
DR InParanoid; Q96NL1; -.
DR OMA; GNPNEWS; -.
DR OrthoDB; 1183825at2759; -.
DR PhylomeDB; Q96NL1; -.
DR TreeFam; TF335735; -.
DR PathwayCommons; Q96NL1; -.
DR SignaLink; Q96NL1; -.
DR BioGRID-ORCS; 157753; 8 hits in 1071 CRISPR screens.
DR ChiTaRS; TMEM74; human.
DR GenomeRNAi; 157753; -.
DR Pharos; Q96NL1; Tbio.
DR PRO; PR:Q96NL1; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q96NL1; protein.
DR Bgee; ENSG00000164841; Expressed in secondary oocyte and 103 other tissues.
DR ExpressionAtlas; Q96NL1; baseline and differential.
DR Genevisible; Q96NL1; HS.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016236; P:macroautophagy; IMP:HGNC.
DR InterPro; IPR029696; Tmem74.
DR InterPro; IPR029695; TMEM74-like.
DR PANTHER; PTHR16125; PTHR16125; 1.
DR PANTHER; PTHR16125:SF3; PTHR16125:SF3; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasmic vesicle; Lysosome; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..305
FT /note="Transmembrane protein 74"
FT /id="PRO_0000259600"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 52..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 305 AA; 33338 MW; 9521A382F126B683 CRC64;
MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP RATEMEGSKL
SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC NCCSQELETS FTYVDKNINL
EQRNRSSPSA KGHNHPGELG WENPNEWSQE AAISLISEEE DDTSSEATSS GKSIDYGFIS
AILFLVTGIL LVIISYIVPR EVTVDPNTVA AREMERLEKE SARLGAHLDR CVIAGLCLLT
LGGVILSCLL MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNENT LELSLVEEDA
LAVQS
