TMM74_MOUSE
ID TMM74_MOUSE Reviewed; 305 AA.
AC Q8BQU7;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Transmembrane protein 74;
GN Name=Tmem74;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays an essential role in autophagy. TMEM74-induced
CC autophagy may involve PI3K signal transduction (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane; Multi-pass membrane protein.
CC Cytoplasmic vesicle, autophagosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TMEM74 family. {ECO:0000305}.
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DR EMBL; AK046420; BAC32721.1; -; mRNA.
DR EMBL; BC117021; AAI17022.1; -; mRNA.
DR EMBL; BC117025; AAI17026.1; -; mRNA.
DR CCDS; CCDS27454.1; -.
DR RefSeq; NP_780711.1; NM_175502.3.
DR RefSeq; XP_006520971.1; XM_006520908.3.
DR AlphaFoldDB; Q8BQU7; -.
DR STRING; 10090.ENSMUSP00000070748; -.
DR iPTMnet; Q8BQU7; -.
DR PhosphoSitePlus; Q8BQU7; -.
DR PaxDb; Q8BQU7; -.
DR PRIDE; Q8BQU7; -.
DR ProteomicsDB; 259262; -.
DR Antibodypedia; 3040; 87 antibodies from 22 providers.
DR DNASU; 239408; -.
DR Ensembl; ENSMUST00000067469; ENSMUSP00000070748; ENSMUSG00000054409.
DR GeneID; 239408; -.
DR KEGG; mmu:239408; -.
DR UCSC; uc007vpn.1; mouse.
DR CTD; 157753; -.
DR MGI; MGI:2443417; Tmem74.
DR VEuPathDB; HostDB:ENSMUSG00000054409; -.
DR eggNOG; ENOG502QUYY; Eukaryota.
DR GeneTree; ENSGT00530000063880; -.
DR HOGENOM; CLU_079915_0_0_1; -.
DR InParanoid; Q8BQU7; -.
DR OMA; GNPNEWS; -.
DR OrthoDB; 1183825at2759; -.
DR PhylomeDB; Q8BQU7; -.
DR TreeFam; TF335735; -.
DR BioGRID-ORCS; 239408; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Tmem74; mouse.
DR PRO; PR:Q8BQU7; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8BQU7; protein.
DR Bgee; ENSMUSG00000054409; Expressed in lumbar dorsal root ganglion and 86 other tissues.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016236; P:macroautophagy; ISO:MGI.
DR InterPro; IPR029696; Tmem74.
DR InterPro; IPR029695; TMEM74-like.
DR PANTHER; PTHR16125; PTHR16125; 1.
DR PANTHER; PTHR16125:SF3; PTHR16125:SF3; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasmic vesicle; Lysosome; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..305
FT /note="Transmembrane protein 74"
FT /id="PRO_0000259601"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 122..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 305 AA; 33416 MW; F53CB0F09DEBEAA9 CRC64;
MELHSLSKRN SPVDPCNALE WSSGETSGDH IEEATIRDAF CYQKNLVSTP RADVVEVCRL
STSPASPTSL LQDSAIQTSF SLSGPPDSGN NQVMADRKVC NCCSQELETS FTYVDENVNL
EQRSQRSPSA KGSNHPVDLG WGNPNEWSHE TAMSLMSEDD DDTSSEATSS GKSVDYGFIS
AILFLVTGIL LVIISYIVPR EVTVDPNTVA AREMERLEKE SAMLGAHLDR CVIAGLCLLT
LGGVVLSCLL MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNEDT LELSLVEEDA
LAVQS
