ID   TMM8B_BOVIN             Reviewed;         472 AA.
AC   A6QLK4; A6QQL1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Transmembrane protein 8B;
DE   AltName: Full=Protein NGX6;
GN   Name=TMEM8B;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Basal ganglia, and Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May function as a regulator of the EGFR pathway. Probable
CC       tumor suppressor which may function in cell growth, proliferation and
CC       adhesion (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: May interact with EZR. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A6NDV4};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:A6NDV4}. Cytoplasm
CC       {ECO:0000250|UniProtKB:A6NDV4}. Nucleus {ECO:0000250|UniProtKB:A6NDV4}.
CC       Mitochondrion {ECO:0000250|UniProtKB:A6NDV4}. Endoplasmic reticulum
CC       {ECO:0000250|UniProtKB:A6NDV4}. Note=Also detected in mitochondrion and
CC       endoplasmic reticulum. {ECO:0000250|UniProtKB:A6NDV4}.
CC   -!- PTM: N-glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TMEM8 family. {ECO:0000305}.
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DR   EMBL; BC147996; AAI47997.1; -; mRNA.
DR   EMBL; BC149881; AAI49882.1; -; mRNA.
DR   RefSeq; NP_001096805.1; NM_001103335.1.
DR   AlphaFoldDB; A6QLK4; -.
DR   STRING; 9913.ENSBTAP00000015212; -.
DR   PaxDb; A6QLK4; -.
DR   PRIDE; A6QLK4; -.
DR   GeneID; 100125302; -.
DR   KEGG; bta:100125302; -.
DR   CTD; 51754; -.
DR   eggNOG; ENOG502QQ7Q; Eukaryota.
DR   HOGENOM; CLU_012979_1_0_1; -.
DR   InParanoid; A6QLK4; -.
DR   OrthoDB; 704708at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR021910; NGX6/PGAP6/MYMK.
DR   PANTHER; PTHR14319; PTHR14319; 1.
DR   Pfam; PF12036; DUF3522; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; 1.
PE   2: Evidence at transcript level;
KW   Cell adhesion; Cell membrane; Cytoplasm; Disulfide bond; EGF-like domain;
KW   Endoplasmic reticulum; Glycoprotein; Growth regulation; Membrane;
KW   Mitochondrion; Nucleus; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..472
FT                   /note="Transmembrane protein 8B"
FT                   /id="PRO_0000333038"
FT   TOPO_DOM        1..233
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        255..257
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        278..292
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        314..315
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        337..342
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        343..363
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        364..379
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        401..405
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        406..426
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        427..472
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          182..221
FT                   /note="EGF-like"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        92
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        100
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        186..196
FT                   /evidence="ECO:0000250"
FT   DISULFID        190..209
FT                   /evidence="ECO:0000250"
FT   DISULFID        211..220
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   472 AA;  52088 MW;  F38114D6D8B9DFE2 CRC64;
     MNMPQSLGNQ PLPPEPPSLR TPAEGPGATS PPEHCWPVRP TLRNELDTFS VHFYIFFGPS
     VALPPERPAV FALRLLPVLD SGGVLSLELQ LNVSSLRQEN VTVFGCLTHE VPLSLGDAAV
     TCSKESLAGF LLTVSAASRV ARLRIPFPQT GTWFLTLRSL CGVGPRFVRC RNATAEVRLR
     TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC
     LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTFYHACDQ PGIVVFCIMD YDVLQFCDFL
     GSLMSVWVTV IAMARLQPVV KQVLYLLGAM LLSMALQLDR HGLWNLLGPS LFALGILATA
     WTVRSVRRRH CYPPTWRRWL FCLCPGSLIA GSAILLYAFV ETRDNYFYIH SIWHMLIAGS
     VGFLLPPRAK TDRRVPSGAR ARGCGYQLCI NEQEELGLVG PGGATVSSIC AS