TMM8B_MOUSE
ID TMM8B_MOUSE Reviewed; 472 AA.
AC B1AWJ5; B9EJV2;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Transmembrane protein 8B;
DE AltName: Full=Protein NGX6;
GN Name=Tmem8b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May function as a regulator of the EGFR pathway. Probable
CC tumor suppressor which may function in cell growth, proliferation and
CC adhesion (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: May interact with EZR. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A6NDV4};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:A6NDV4}. Cytoplasm
CC {ECO:0000250|UniProtKB:A6NDV4}. Nucleus {ECO:0000250|UniProtKB:A6NDV4}.
CC Mitochondrion {ECO:0000250|UniProtKB:A6NDV4}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:A6NDV4}. Note=Also detected in mitochondrion and
CC endoplasmic reticulum. {ECO:0000250|UniProtKB:A6NDV4}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TMEM8 family. {ECO:0000305}.
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DR EMBL; AL732626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC147569; AAI47570.1; -; mRNA.
DR EMBL; BC147574; AAI47575.1; -; mRNA.
DR EMBL; BC151067; AAI51068.1; -; mRNA.
DR EMBL; BC151070; AAI51071.1; -; mRNA.
DR CCDS; CCDS51168.1; -.
DR RefSeq; NP_001078977.1; NM_001085508.2.
DR RefSeq; XP_011248332.1; XM_011250030.2.
DR AlphaFoldDB; B1AWJ5; -.
DR STRING; 10090.ENSMUSP00000103497; -.
DR GlyGen; B1AWJ5; 1 site.
DR iPTMnet; B1AWJ5; -.
DR PhosphoSitePlus; B1AWJ5; -.
DR PaxDb; B1AWJ5; -.
DR PRIDE; B1AWJ5; -.
DR ProteomicsDB; 259435; -.
DR Antibodypedia; 26101; 155 antibodies from 20 providers.
DR Ensembl; ENSMUST00000107864; ENSMUSP00000103496; ENSMUSG00000078716.
DR Ensembl; ENSMUST00000107865; ENSMUSP00000103497; ENSMUSG00000078716.
DR Ensembl; ENSMUST00000167153; ENSMUSP00000129760; ENSMUSG00000078716.
DR GeneID; 242409; -.
DR KEGG; mmu:242409; -.
DR UCSC; uc008sqv.1; mouse.
DR CTD; 51754; -.
DR MGI; MGI:2441680; Tmem8b.
DR VEuPathDB; HostDB:ENSMUSG00000078716; -.
DR eggNOG; ENOG502QQ7Q; Eukaryota.
DR GeneTree; ENSGT00940000157861; -.
DR HOGENOM; CLU_012979_1_0_1; -.
DR InParanoid; B1AWJ5; -.
DR OMA; LDCREWN; -.
DR PhylomeDB; B1AWJ5; -.
DR TreeFam; TF331003; -.
DR BioGRID-ORCS; 242409; 0 hits in 72 CRISPR screens.
DR PRO; PR:B1AWJ5; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; B1AWJ5; protein.
DR Bgee; ENSMUSG00000078716; Expressed in spermatid and 58 other tissues.
DR ExpressionAtlas; B1AWJ5; baseline and differential.
DR Genevisible; B1AWJ5; MM.
DR GO; GO:0009986; C:cell surface; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0007160; P:cell-matrix adhesion; ISO:MGI.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR021910; NGX6/PGAP6/MYMK.
DR PANTHER; PTHR14319; PTHR14319; 1.
DR Pfam; PF12036; DUF3522; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS01186; EGF_2; 1.
PE 2: Evidence at transcript level;
KW Cell adhesion; Cell membrane; Cytoplasm; Disulfide bond; EGF-like domain;
KW Endoplasmic reticulum; Glycoprotein; Growth regulation; Membrane;
KW Mitochondrion; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..472
FT /note="Transmembrane protein 8B"
FT /id="PRO_0000333040"
FT TOPO_DOM 1..233
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..292
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..315
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..379
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..405
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..472
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 182..221
FT /note="EGF-like"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 186..196
FT /evidence="ECO:0000250"
FT DISULFID 190..209
FT /evidence="ECO:0000250"
FT DISULFID 211..220
FT /evidence="ECO:0000250"
SQ SEQUENCE 472 AA; 52114 MW; 7CA09DD6D1676DDE CRC64;
MNMPQSLGTQ PLPPEPPSLG TPIEGSGAIA PTEHCWPVRP TLRNELDTFS VHFYIFFGPS
VALPPERPAV FALRLLPVLD SGGVLSLELQ LNASSLRQEN VTVFGCLTHE VPLSLGDAAV
TCSKESLAGF LLSVSATSRV ARLRIPFPQT GTWFLTLRSL CGVGPRFVRC RNATAEVRLR
TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC
LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTFYHACDQ PGIVVFCIMD YDVLQFCDFL
GSLMSVWVTV IAMARLQPVI KQVLYLLGAM LLSMALQLDR HGLWNLLGPS LFALGILATA
WTVRSVRRRH CYPPTWRRWL FYLCPGSLIA GSAVLLYAFV ETRDNYFYIH SIWHMLIAGS
VGFLLPPRAK TDRRVPSGAR ARGCGYQLCI NEQEELGLVG PGGTTVSSIC VS
