TMM95_BOVIN
ID TMM95_BOVIN Reviewed; 166 AA.
AC A0A3Q1LRJ2; A0A0N9GZ96;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-OCT-2020, sequence version 2.
DT 03-AUG-2022, entry version 15.
DE RecName: Full=Sperm-egg fusion protein TMEM95 {ECO:0000305};
DE AltName: Full=Transmembrane protein 95 {ECO:0000250|UniProtKB:Q3KNT9};
DE Flags: Precursor;
GN Name=TMEM95 {ECO:0000250|UniProtKB:Q3KNT9};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN [1] {ECO:0000312|EMBL:ALF95170.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Qinchuan {ECO:0000303|PubMed:26385321};
RX PubMed=26385321; DOI=10.1016/j.gene.2015.09.026;
RA Zhang S., Cai H., Yang Q., Shi T., Pan C., Lei C., Dang R., Chen H.,
RA Lan X.;
RT "Identification of novel alternative splicing transcript and expression
RT analysis of bovine TMEM95 gene.";
RL Gene 575:531-536(2016).
RN [2] {ECO:0000312|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000312|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND VARIANT 152-CYS--GLU-166 DEL.
RC STRAIN=Fleckvieh {ECO:0000303|PubMed:24391514};
RX PubMed=24391514; DOI=10.1371/journal.pgen.1004044;
RA Pausch H., Koelle S., Wurmser C., Schwarzenbacher H., Emmerling R.,
RA Jansen S., Trottmann M., Fuerst C., Goetz K.U., Fries R.;
RT "A nonsense mutation in TMEM95 encoding a nondescript transmembrane protein
RT causes idiopathic male subfertility in cattle.";
RL PLoS Genet. 10:e1004044-e1004044(2014).
RN [4] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND CHARACTERIZATION OF VARIANT 152-CYS--GLU-166 DEL.
RX PubMed=28859280; DOI=10.1093/biolre/iox065;
RA Fernandez-Fuertes B., Laguna-Barraza R., Fernandez-Gonzalez R.,
RA Gutierrez-Adan A., Blanco-Fernandez A., O'Doherty A.M., Di Fenza M.,
RA Kelly A.K., Koelle S., Lonergan P.;
RT "Subfertility in bulls carrying a nonsense mutation in transmembrane
RT protein 95 is due to failure to interact with the oocyte vestments.";
RL Biol. Reprod. 97:50-60(2017).
RN [5] {ECO:0000305}
RP ABSENCE OF VARIANT 152-CYS--GLU-166 DEL IN CHINESE CATTLE BREEDS.
RX PubMed=31315171; DOI=10.3390/ani9070444;
RA Zhang S., Peng K., Zhang G., Cao Y., Zhang M., Chen H., Lei C., Lan X.,
RA Zhao Y.;
RT "Detection of Bovine TMEM95 p.Cys161X Mutation in 13 Chinese Indigenous
RT Cattle Breeds.";
RL Animals 9:444-444(2019).
CC -!- FUNCTION: Sperm protein required for fusion of sperm with the egg
CC membrane during fertilization. {ECO:0000250|UniProtKB:P0DJF3}.
CC -!- SUBUNIT: Does not interact with sperm-egg fusion proteins IZUMO1 or
CC IZUMO1R/JUNO. {ECO:0000250|UniProtKB:P0DJF3}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC membrane {ECO:0000269|PubMed:24391514, ECO:0000269|PubMed:28859280};
CC Single-pass type I membrane protein {ECO:0000305}. Note=Following the
CC acrosome reaction, relocalizes to the equatorial segment.
CC {ECO:0000250|UniProtKB:P0DJF3}.
CC -!- TISSUE SPECIFICITY: Detected in testis and brain with higher levels in
CC brain than testis. {ECO:0000269|PubMed:26385321}.
CC -!- SIMILARITY: Belongs to the TMEM95 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ALF95170.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; KT200428; ALF95170.1; ALT_INIT; mRNA.
DR AlphaFoldDB; A0A3Q1LRJ2; -.
DR VEuPathDB; HostDB:ENSBTAG00000052395; -.
DR Proteomes; UP000009136; Chromosome 19.
DR GO; GO:0002080; C:acrosomal membrane; IDA:UniProtKB.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0097524; C:sperm plasma membrane; IEA:InterPro.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; ISS:UniProtKB.
DR GO; GO:0007338; P:single fertilization; IMP:UniProtKB.
DR InterPro; IPR027984; TMEM95.
DR PANTHER; PTHR38808; PTHR38808; 1.
DR Pfam; PF15203; TMEM95; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Fertilization; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..166
FT /note="Sperm-egg fusion protein TMEM95"
FT /evidence="ECO:0000255"
FT /id="PRO_0000450825"
FT TOPO_DOM 17..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 152..166
FT /note="Missing (in strain: Fleckvieh; associated with male
FT subfertility; does not affect sperm morphology or motility;
FT reduced interaction with the zona pellucida; not detected
FT in a study of 765 individuals from 13 indigenous Chinese
FT cattle breeds)"
FT /evidence="ECO:0000269|PubMed:24391514,
FT ECO:0000269|PubMed:28859280, ECO:0000269|PubMed:31315171"
FT CONFLICT 75
FT /note="M -> V (in Ref. 1; ALF95170)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="R -> Q (in Ref. 1; ALF95170)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="R -> L (in Ref. 1; ALF95170)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="S -> A (in Ref. 1; ALF95170)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 166 AA; 19042 MW; 87E0803FCB6B80EE CRC64;
MWTLALGGIF LAAVEACVFC RFPDRELSGR LARLCSQMEV QWKDCEVSWT FSAFALDDAS
LNKITEKTHR VLRVMEIKGS LYSLPSYWQW LRKTKLREYN REALCPPSCR GSTILYNCST
CQGFEVYCWP RKRCFPGSHD LWEARILLLF VCGTALLLGV PSLAVE
