TMM95_MOUSE
ID TMM95_MOUSE Reviewed; 176 AA.
AC P0DJF3;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Sperm-egg fusion protein TMEM95 {ECO:0000305};
DE AltName: Full=Transmembrane protein 95 {ECO:0000312|MGI:MGI:3779488};
DE Flags: Precursor;
GN Name=Tmem95 {ECO:0000312|MGI:MGI:3779488};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 19-152.
RC STRAIN=CD-1; TISSUE=Spermatid;
RA Marra M., Hillier L., Kucaba T., Martin J., Beck C., Wylie T.,
RA Underwood K., Steptoe M., Theising B., Allen M., Bowers Y., Person B.,
RA Swaller T., Gibbons M., Pape D., Harvey N., Schurk R., Ritter E., Kohn S.,
RA Shin T., Jackson Y., Cardenas M., McCann R., Waterston R., Wilson R.;
RT "The WashU-NCI mouse EST project 1999.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, LACK OF INTERACTION WITH IZUMO1 AND IZUMO1R, SUBCELLULAR
RP LOCATION, DISRUPTION PHENOTYPE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=32484434; DOI=10.7554/elife.53913;
RA Lamas-Toranzo I., Hamze J.G., Bianchi E., Fernandez-Fuertes B.,
RA Perez-Cerezales S., Laguna-Barraza R., Fernandez-Gonzalez R., Lonergan P.,
RA Gutierrez-Adan A., Wright G.J., Jimenez-Movilla M., Bermejo-Alvarez P.;
RT "TMEM95 is a sperm membrane protein essential for mammalian
RT fertilization.";
RL Elife 9:0-0(2020).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=32393636; DOI=10.1073/pnas.1922650117;
RA Noda T., Lu Y., Fujihara Y., Oura S., Koyano T., Kobayashi S., Matzuk M.M.,
RA Ikawa M.;
RT "Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte
RT fusion in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:11493-11502(2020).
CC -!- FUNCTION: Sperm protein required for fusion of sperm with the egg
CC membrane during fertilization. {ECO:0000269|PubMed:32393636,
CC ECO:0000269|PubMed:32484434}.
CC -!- SUBUNIT: Does not interact with sperm-egg fusion proteins IZUMO1 or
CC IZUMO1R/JUNO. {ECO:0000269|PubMed:32484434}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC membrane {ECO:0000269|PubMed:32484434}; Single-pass type I membrane
CC protein {ECO:0000305}. Note=Following the acrosome reaction,
CC relocalizes to the equatorial segment. {ECO:0000269|PubMed:32484434}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in testis.
CC {ECO:0000269|PubMed:32393636}.
CC -!- DEVELOPMENTAL STAGE: Expression is first detected at postnatal day 21.
CC {ECO:0000269|PubMed:32393636}.
CC -!- DISRUPTION PHENOTYPE: Males are healthy, grossly normal and exhibit
CC normal mating behavior but are infertile (PubMed:32484434,
CC PubMed:32393636). Sperm are morphologically normal, exhibit normal
CC motility and can penetrate the zona pellucida and bind to the oolemma
CC but are unable to fuse with the egg membrane or penetrate into the
CC ooplasm (PubMed:32484434, PubMed:32393636). No effect on location of
CC sperm-egg fusion protein IZUMO1 (PubMed:32393636).
CC {ECO:0000269|PubMed:32393636, ECO:0000269|PubMed:32484434}.
CC -!- SIMILARITY: Belongs to the TMEM95 family. {ECO:0000305}.
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DR EMBL; AL845465; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BF018485; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS56783.1; -.
DR RefSeq; NP_001182639.1; NM_001195710.1.
DR AlphaFoldDB; P0DJF3; -.
DR STRING; 10090.ENSMUSP00000137492; -.
DR GlyGen; P0DJF3; 2 sites.
DR PhosphoSitePlus; P0DJF3; -.
DR PaxDb; P0DJF3; -.
DR PRIDE; P0DJF3; -.
DR ProteomicsDB; 259437; -.
DR Antibodypedia; 76550; 28 antibodies from 5 providers.
DR Ensembl; ENSMUST00000178597; ENSMUSP00000137492; ENSMUSG00000094845.
DR GeneID; 100503361; -.
DR KEGG; mmu:100503361; -.
DR UCSC; uc011xxc.1; mouse.
DR CTD; 339168; -.
DR MGI; MGI:3779488; Tmem95.
DR VEuPathDB; HostDB:ENSMUSG00000094845; -.
DR eggNOG; ENOG502S64R; Eukaryota.
DR GeneTree; ENSGT00390000018162; -.
DR HOGENOM; CLU_130463_0_0_1; -.
DR InParanoid; P0DJF3; -.
DR OMA; WPRKRCL; -.
DR OrthoDB; 1385077at2759; -.
DR TreeFam; TF342122; -.
DR BioGRID-ORCS; 100503361; 1 hit in 71 CRISPR screens.
DR PRO; PR:P0DJF3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P0DJF3; protein.
DR Bgee; ENSMUSG00000094845; Expressed in testis and 34 other tissues.
DR GO; GO:0002080; C:acrosomal membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0097524; C:sperm plasma membrane; IEA:InterPro.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IMP:UniProtKB.
DR InterPro; IPR027984; TMEM95.
DR PANTHER; PTHR38808; PTHR38808; 1.
DR Pfam; PF15203; TMEM95; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Fertilization; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..176
FT /note="Sperm-egg fusion protein TMEM95"
FT /id="PRO_0000416121"
FT TOPO_DOM 17..146
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 176 AA; 20158 MW; A66F8EA8C3F99FAD CRC64;
MWVLALGGAF LAVAKACIFC RLQDHALANR LAQLNNQTKP KWKWKEWASP DFSAFALDEV
SMKQVTEKTH RVLRVIEKKG STSLIPLYWQ WLQKTRIPQY TREALCAPVC RGSTILYNCS
TCEGKEESCW PQKHCYPDSH DLWDARILLL CIFGIVLLSG VVSLQVEYLN LQAKDL
