TMM9B_HUMAN
ID TMM9B_HUMAN Reviewed; 198 AA.
AC Q9NQ34; Q7Z649;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transmembrane protein 9B;
DE Flags: Precursor;
GN Name=TMEM9B; Synonyms=C11orf15; ORFNames=UNQ712/PRO1375;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11528127; DOI=10.1159/000056999;
RA Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A.,
RA Zabel B., Hankeln T., Schmidt E.R.;
RT "Comparative genomic sequencing reveals a strikingly similar architecture
RT of a conserved syntenic region on human chromosome 11p15.3 (including gene
RT ST5) and mouse chromosome 7.";
RL Cytogenet. Cell Genet. 93:284-290(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Pancreas, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 34-48.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX PubMed=18541524; DOI=10.1074/jbc.m801908200;
RA Dodeller F., Gottar M., Huesken D., Iourgenko V., Cenni B.;
RT "The lysosomal transmembrane protein 9B regulates the activity of
RT inflammatory signaling pathways.";
RL J. Biol. Chem. 283:21487-21494(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Enhances production of pro-inflammatory cytokines induced by
CC TNF, IL1B, and TLR ligands. Has a role in TNF activation of both the
CC NF-kappaB and MAPK pathways. {ECO:0000269|PubMed:18541524}.
CC -!- INTERACTION:
CC Q9NQ34; O75344: FKBP6; NbExp=3; IntAct=EBI-724458, EBI-744771;
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:18541524};
CC Single-pass membrane protein {ECO:0000255}. Early endosome membrane
CC {ECO:0000269|PubMed:18541524}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NQ34-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NQ34-2; Sequence=VSP_055294;
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:18541524}.
CC -!- SIMILARITY: Belongs to the TMEM9 family. {ECO:0000305}.
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DR EMBL; AJ400877; CAB92287.1; -; Genomic_DNA.
DR EMBL; AY359069; AAQ89428.1; -; mRNA.
DR EMBL; AK316216; BAH14587.1; -; mRNA.
DR EMBL; AC026894; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471064; EAW68608.1; -; Genomic_DNA.
DR EMBL; CH471064; EAW68609.1; -; Genomic_DNA.
DR EMBL; BC015884; AAH15884.1; -; mRNA.
DR EMBL; BC040124; AAH40124.1; -; mRNA.
DR CCDS; CCDS66021.1; -. [Q9NQ34-2]
DR CCDS; CCDS7796.1; -. [Q9NQ34-1]
DR RefSeq; NP_001273023.1; NM_001286094.1. [Q9NQ34-2]
DR RefSeq; NP_001273024.1; NM_001286095.1. [Q9NQ34-2]
DR RefSeq; NP_065695.1; NM_020644.2. [Q9NQ34-1]
DR AlphaFoldDB; Q9NQ34; -.
DR BioGRID; 121182; 130.
DR IntAct; Q9NQ34; 26.
DR STRING; 9606.ENSP00000433361; -.
DR GlyGen; Q9NQ34; 3 sites.
DR iPTMnet; Q9NQ34; -.
DR PhosphoSitePlus; Q9NQ34; -.
DR BioMuta; TMEM9B; -.
DR DMDM; 20532029; -.
DR EPD; Q9NQ34; -.
DR jPOST; Q9NQ34; -.
DR MassIVE; Q9NQ34; -.
DR MaxQB; Q9NQ34; -.
DR PaxDb; Q9NQ34; -.
DR PeptideAtlas; Q9NQ34; -.
DR PRIDE; Q9NQ34; -.
DR ProteomicsDB; 69374; -.
DR ProteomicsDB; 82069; -. [Q9NQ34-1]
DR Antibodypedia; 48632; 20 antibodies from 11 providers.
DR DNASU; 56674; -.
DR Ensembl; ENST00000309134.9; ENSP00000311842.5; ENSG00000175348.11. [Q9NQ34-2]
DR Ensembl; ENST00000525069.5; ENSP00000431487.1; ENSG00000175348.11. [Q9NQ34-2]
DR Ensembl; ENST00000534025.6; ENSP00000433361.1; ENSG00000175348.11. [Q9NQ34-1]
DR GeneID; 56674; -.
DR KEGG; hsa:56674; -.
DR MANE-Select; ENST00000534025.6; ENSP00000433361.1; NM_020644.3; NP_065695.1.
DR UCSC; uc001mhe.3; human. [Q9NQ34-1]
DR CTD; 56674; -.
DR DisGeNET; 56674; -.
DR GeneCards; TMEM9B; -.
DR HGNC; HGNC:1168; TMEM9B.
DR HPA; ENSG00000175348; Low tissue specificity.
DR neXtProt; NX_Q9NQ34; -.
DR OpenTargets; ENSG00000175348; -.
DR PharmGKB; PA25482; -.
DR VEuPathDB; HostDB:ENSG00000175348; -.
DR eggNOG; KOG4007; Eukaryota.
DR GeneTree; ENSGT00390000000819; -.
DR HOGENOM; CLU_1964436_0_0_1; -.
DR InParanoid; Q9NQ34; -.
DR OMA; CKYESRN; -.
DR PhylomeDB; Q9NQ34; -.
DR TreeFam; TF315146; -.
DR PathwayCommons; Q9NQ34; -.
DR SignaLink; Q9NQ34; -.
DR BioGRID-ORCS; 56674; 13 hits in 1082 CRISPR screens.
DR ChiTaRS; TMEM9B; human.
DR GenomeRNAi; 56674; -.
DR Pharos; Q9NQ34; Tdark.
DR PRO; PR:Q9NQ34; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9NQ34; protein.
DR Bgee; ENSG00000175348; Expressed in esophagus squamous epithelium and 199 other tissues.
DR ExpressionAtlas; Q9NQ34; baseline and differential.
DR Genevisible; Q9NQ34; HS.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; HMP:UniProtKB.
DR InterPro; IPR008853; TMEM9/TMEM9B.
DR PANTHER; PTHR13064; PTHR13064; 1.
DR Pfam; PF05434; Tmemb_9; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Endosome; Glycoprotein;
KW Lysosome; Membrane; Phosphoprotein; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..33
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 34..198
FT /note="Transmembrane protein 9B"
FT /id="PRO_0000034376"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9JJR8"
FT MOD_RES 189
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..74
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_055294"
SQ SEQUENCE 198 AA; 22531 MW; 888E55365A01B44F CRC64;
MATLWGGLLR LGSLLSLSCL ALSVLLLAQL SDAAKNFEDV RCKCICPPYK ENSGHIYNKN
ISQKDCDCLH VVEPMPVRGP DVEAYCLRCE CKYEERSSVT IKVTIIIYLS ILGLLLLYMV
YLTLVEPILK RRLFGHAQLI QSDDDIGDHQ PFANAHDVLA RSRSRANVLN KVEYAQQRWK
LQVQEQRKSV FDRHVVLS
