TMN10_ARATH
ID TMN10_ARATH Reviewed; 637 AA.
AC Q8RWW1; Q9ZUH6;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Transmembrane 9 superfamily member 10 {ECO:0000305};
DE AltName: Full=Endomembrane protein 3 {ECO:0000303|PubMed:22570441};
DE AltName: Full=Transmembrane nine protein 10 {ECO:0000303|PubMed:20681974};
DE Short=AtTMN10 {ECO:0000303|PubMed:20681974};
DE Flags: Precursor;
GN Name=TMN10 {ECO:0000303|PubMed:20681974};
GN Synonyms=EMP3 {ECO:0000303|PubMed:22570441};
GN OrderedLocusNames=At2g24170 {ECO:0000312|Araport:AT2G24170};
GN ORFNames=F27D4.8 {ECO:0000312|EMBL:AAD03378.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22570441; DOI=10.1105/tpc.112.096057;
RA Gao C., Yu C.K., Qu S., San M.W., Li K.Y., Lo S.W., Jiang L.;
RT "The Golgi-localized Arabidopsis endomembrane protein12 contains both
RT endoplasmic reticulum export and Golgi retention signals at its C
RT terminus.";
RL Plant Cell 24:2086-2104(2012).
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P32802};
CC Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q940G0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: The C-terminal KXD/E motif functions as a Golgi retention
CC signal, certainly through the binding to the COP1 coatomer.
CC {ECO:0000250|UniProtKB:Q940G0}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD03378.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005967; AAD03378.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07535.1; -; Genomic_DNA.
DR EMBL; AY091067; AAM13887.1; -; mRNA.
DR PIR; D84633; D84633.
DR RefSeq; NP_179994.2; NM_127978.4.
DR AlphaFoldDB; Q8RWW1; -.
DR BioGRID; 2303; 26.
DR IntAct; Q8RWW1; 24.
DR STRING; 3702.AT2G24170.1; -.
DR PaxDb; Q8RWW1; -.
DR PRIDE; Q8RWW1; -.
DR ProteomicsDB; 234437; -.
DR EnsemblPlants; AT2G24170.1; AT2G24170.1; AT2G24170.
DR GeneID; 816951; -.
DR Gramene; AT2G24170.1; AT2G24170.1; AT2G24170.
DR KEGG; ath:AT2G24170; -.
DR Araport; AT2G24170; -.
DR TAIR; locus:2047565; AT2G24170.
DR eggNOG; KOG1278; Eukaryota.
DR HOGENOM; CLU_010714_4_1_1; -.
DR InParanoid; Q8RWW1; -.
DR OMA; RWDLYFV; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q8RWW1; -.
DR PRO; PR:Q8RWW1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8RWW1; baseline and differential.
DR Genevisible; Q8RWW1; AT.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 2: Evidence at transcript level;
KW Endosome; Golgi apparatus; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..637
FT /note="Transmembrane 9 superfamily member 10"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431267"
FT TOPO_DOM 24..274
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 275..295
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..344
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 345..365
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..370
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 371..391
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 412..432
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..444
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 445..465
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 466..494
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 495..515
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..527
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 528..548
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 549..566
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 567..587
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 588..593
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 594..614
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 615..637
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 626..631
FT /note="Endoplasmic reticulum export signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
FT MOTIF 635..637
FT /note="Golgi retention signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
SQ SEQUENCE 637 AA; 73352 MW; 6FABA118459E2533 CRC64;
MAKVRILIFT LVLFFSLNVH IHGFYLPGVA PQDFQMGDAL MVKVNKLTST KTQLPYSYYS
LPYCRPEHIV DSAENLGEVL RGDRIENSPF VFKMRESQMC AAVCRVKLDK KTAKAFKEKI
ADEYRVNMIL DNLPLVVPVQ RPDQDNVVVY QHGFHVGLKG IFAGKKEEKY FIHNHLTFTV
RYHRDIQTDS SRIVGFEVKP FSVKHEYEGQ WNEKARLTTC DPHTKRAVTN SESPQEVEEG
NEIIFTYDVD FQESEVKWAS RWDTYLLMAD DQIHWFSIVN SMMIVLFLSG MVAMIMLRTL
YRDISNYNQL ESHEEALEET GWKLVHGDVF RPPTNPELLC VYAGTGVQCF GMILVTMIFA
CLGFLSPSNR GGLMTAMLLL WVFMGLLAGY ASSRLYKTLR GTEWKRNALK TAFMFPATVF
VAFFVLNAII WGQKSSGAVP FGTMFALVVL WFGISVPLVF IGGYIGFRKP APEDPVKTNK
IPRQIPTQAW YMNPIFSILI GGILPFGAVF IELFFILTSI WLHQFYYIFG FLFIVFIILI
ITCAEITVVL CYFQLCSEDY QWWWRSYLTS GSSAVYLFLY AVFYFYTKLE ITKLVSAVLY
FGYMLIVSYV FFVFTGAIGF YACFWFTRLI YSSVKID
